Diagnosis of human brucellosis in Egypt by PCR
Background: Brucellosis is a significant health problem in Egypt.Methodology: To establish a PCR technique for diagnosis of active brucellosis in our institutions, DNA extraction was done using a commercial kit, and a laboratory extraction procedure. PCR amplification was done using 2 sets of primer...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2007-10-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | http://www.oloep.org/jidc/content.asp?id=1092 |
| _version_ | 1818573945979273216 |
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| author | El Kholy Amani Ali,1 Gomaa Howayda Ezzeldin,2 El Anany Mervat Gaber,1 Abd El Rasheed Enas2 |
| author_facet | El Kholy Amani Ali,1 Gomaa Howayda Ezzeldin,2 El Anany Mervat Gaber,1 Abd El Rasheed Enas2 |
| author_sort | El Kholy Amani Ali,1 Gomaa Howayda Ezzeldin,2 El Anany Mervat Gaber,1 Abd El Rasheed Enas2 |
| collection | DOAJ |
| description | Background: Brucellosis is a significant health problem in Egypt.Methodology: To establish a PCR technique for diagnosis of active brucellosis in our institutions, DNA extraction was done using a commercial kit, and a laboratory extraction procedure. PCR amplification was done using 2 sets of primers: B4/B5 and JPF/JPR. Extraction of Brucella DNA using the commercial kit failed; therefore, this technique was discontinued. The laboratory extraction method was successful and more economic.Results: The technique showed high sensitivity, specificity, and accuracy. The PCR positivity increased significantly (P=0.001) with increasing the positive standard tube agglutination titer and showed 100% positivity in patients with positive blood culture.Conclusions: An accurate and sensitive PCR technique was established. Based on the results, the PCR method is recommended as an alternative to culture for the diagnosis of brucellosis. A large-scale study to evaluate this PCR technique to screen for brucellosis in Egypt is needed. |
| first_indexed | 2024-12-15T00:19:51Z |
| format | Article |
| id | doaj.art-0ad3624e68cc4c329c9313d57565fb67 |
| institution | Directory Open Access Journal |
| issn | 1972-2680 |
| language | English |
| last_indexed | 2024-12-15T00:19:51Z |
| publishDate | 2007-10-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj.art-0ad3624e68cc4c329c9313d57565fb672022-12-21T22:42:20ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802007-10-0112177181Diagnosis of human brucellosis in Egypt by PCREl Kholy Amani Ali,1 Gomaa Howayda Ezzeldin,2 El Anany Mervat Gaber,1 Abd El Rasheed Enas2Background: Brucellosis is a significant health problem in Egypt.Methodology: To establish a PCR technique for diagnosis of active brucellosis in our institutions, DNA extraction was done using a commercial kit, and a laboratory extraction procedure. PCR amplification was done using 2 sets of primers: B4/B5 and JPF/JPR. Extraction of Brucella DNA using the commercial kit failed; therefore, this technique was discontinued. The laboratory extraction method was successful and more economic.Results: The technique showed high sensitivity, specificity, and accuracy. The PCR positivity increased significantly (P=0.001) with increasing the positive standard tube agglutination titer and showed 100% positivity in patients with positive blood culture.Conclusions: An accurate and sensitive PCR technique was established. Based on the results, the PCR method is recommended as an alternative to culture for the diagnosis of brucellosis. A large-scale study to evaluate this PCR technique to screen for brucellosis in Egypt is needed.http://www.oloep.org/jidc/content.asp?id=1092BrucellosisPCREgypt |
| spellingShingle | El Kholy Amani Ali,1 Gomaa Howayda Ezzeldin,2 El Anany Mervat Gaber,1 Abd El Rasheed Enas2 Diagnosis of human brucellosis in Egypt by PCR Journal of Infection in Developing Countries Brucellosis PCR Egypt |
| title | Diagnosis of human brucellosis in Egypt by PCR |
| title_full | Diagnosis of human brucellosis in Egypt by PCR |
| title_fullStr | Diagnosis of human brucellosis in Egypt by PCR |
| title_full_unstemmed | Diagnosis of human brucellosis in Egypt by PCR |
| title_short | Diagnosis of human brucellosis in Egypt by PCR |
| title_sort | diagnosis of human brucellosis in egypt by pcr |
| topic | Brucellosis PCR Egypt |
| url | http://www.oloep.org/jidc/content.asp?id=1092 |
| work_keys_str_mv | AT elkholyamaniali1gomaahowaydaezzeldin2elananymervatgaber1abdelrasheedenas2 diagnosisofhumanbrucellosisinegyptbypcr |