Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity
In this paper nickel oxide (NiO) nanoflower was synthesized and used for the realization of direct electrochemistry of horseradish peroxidase (HRP). By using carbon ionic liquid electrode (CILE) as the substrate electrode, NiO-HRP composite was casted on the surface of CILE with chitosan (CTS) as th...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Croatian Chemical Society
2016-09-01
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| Series: | Croatica Chemica Acta |
| Online Access: | http://hrcak.srce.hr/file/251140 |
| _version_ | 1818981545291022336 |
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| author | Lijun Yan Xiaofeng Wang Qiutong Li Wencheng Wang Shixing Gong Zheng You Peng Yi Wei Sun |
| author_facet | Lijun Yan Xiaofeng Wang Qiutong Li Wencheng Wang Shixing Gong Zheng You Peng Yi Wei Sun |
| author_sort | Lijun Yan |
| collection | DOAJ |
| description | In this paper nickel oxide (NiO) nanoflower was synthesized and used for the realization of direct electrochemistry of horseradish peroxidase (HRP). By using carbon ionic liquid electrode (CILE) as the substrate electrode, NiO-HRP composite was casted on the surface of CILE with chitosan (CTS) as the film forming material and the modified electrode was denoted as CTS/NiO-HRP/CILE. UV-Vis absorption and FT-IR spectra confirmed that HRP retained its native structure after mixed with NiO nanoflower. Direct electron transfer of HRP on the modified electrode was investigated by cyclic voltammetry with a pair of quasi-reversible redox waves appeared, indicating that the presence of NiO nanoflower on the electrode surface could accelerate the electron transfer rate between the electroactive center of HRP and the substrate electrode. Electrochemical behaviors of HRP on the modified electrode were carefully investigated. The HRP modified electrode showed excellent electrocatalytic activity to the reduction of trichloroacetic acid with wider linear range and lower detection limit. Therefore the presence of NiO nanoflower could provide a friendly biocompatible interface for immobilizing biomolecules and keeping their native structure. The fabricated electrochemical biosensor displayed the advantages such as high sensitivity, good reproducibility and long-term stability.
<br><a rel="license" href="http://creativecommons.org/licenses/by/4.0/"><img alt="Creative Commons License" style="border-width:0" src="https://i.creativecommons.org/l/by/4.0/80x15.png" /></a> This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License</a>. |
| first_indexed | 2024-12-20T17:33:01Z |
| format | Article |
| id | doaj.art-0ad534b02d3e4d849596ffe7703f0eb8 |
| institution | Directory Open Access Journal |
| issn | 0011-1643 1334-417X |
| language | English |
| last_indexed | 2024-12-20T17:33:01Z |
| publishDate | 2016-09-01 |
| publisher | Croatian Chemical Society |
| record_format | Article |
| series | Croatica Chemica Acta |
| spelling | doaj.art-0ad534b02d3e4d849596ffe7703f0eb82022-12-21T19:31:19ZengCroatian Chemical SocietyCroatica Chemica Acta0011-16431334-417X2016-09-0189333133710.5562/cca2717170288Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic ActivityLijun Yan0Xiaofeng Wang1Qiutong Li2Wencheng Wang3Shixing Gong4Zheng You5Peng Yi6Wei Sun7Key Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. ChinaDepartment of Precision Instruments, Tsinghua University, Beijing 100084, P. R. ChinaKey Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. ChinaKey Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. ChinaKey Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. ChinaDepartment of Precision Instruments, Tsinghua University, Beijing 100084, P. R. ChinaKey Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. ChinaKey Laboratory of Tropical Medicinal Plant Chemistry of Ministry of Education, College of Chemistry and Chemical Engineering, Hainan Normal University, Haikou 571158, P. R. ChinaIn this paper nickel oxide (NiO) nanoflower was synthesized and used for the realization of direct electrochemistry of horseradish peroxidase (HRP). By using carbon ionic liquid electrode (CILE) as the substrate electrode, NiO-HRP composite was casted on the surface of CILE with chitosan (CTS) as the film forming material and the modified electrode was denoted as CTS/NiO-HRP/CILE. UV-Vis absorption and FT-IR spectra confirmed that HRP retained its native structure after mixed with NiO nanoflower. Direct electron transfer of HRP on the modified electrode was investigated by cyclic voltammetry with a pair of quasi-reversible redox waves appeared, indicating that the presence of NiO nanoflower on the electrode surface could accelerate the electron transfer rate between the electroactive center of HRP and the substrate electrode. Electrochemical behaviors of HRP on the modified electrode were carefully investigated. The HRP modified electrode showed excellent electrocatalytic activity to the reduction of trichloroacetic acid with wider linear range and lower detection limit. Therefore the presence of NiO nanoflower could provide a friendly biocompatible interface for immobilizing biomolecules and keeping their native structure. The fabricated electrochemical biosensor displayed the advantages such as high sensitivity, good reproducibility and long-term stability. <br><a rel="license" href="http://creativecommons.org/licenses/by/4.0/"><img alt="Creative Commons License" style="border-width:0" src="https://i.creativecommons.org/l/by/4.0/80x15.png" /></a> This work is licensed under a <a rel="license" href="http://creativecommons.org/licenses/by/4.0/">Creative Commons Attribution 4.0 International License</a>.http://hrcak.srce.hr/file/251140 |
| spellingShingle | Lijun Yan Xiaofeng Wang Qiutong Li Wencheng Wang Shixing Gong Zheng You Peng Yi Wei Sun Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity Croatica Chemica Acta |
| title | Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity |
| title_full | Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity |
| title_fullStr | Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity |
| title_full_unstemmed | Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity |
| title_short | Direct Electrochemistry of Horseradish Peroxidase on NiO Nanoflower Modified Electrode and Its Electrocatalytic Activity |
| title_sort | direct electrochemistry of horseradish peroxidase on nio nanoflower modified electrode and its electrocatalytic activity |
| url | http://hrcak.srce.hr/file/251140 |
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