MICROSATELLITE DIVERSITY OF CROSSBRED HORSES RAISED IN BOSNIA AND HERZEGOVINA
The focus of this study was microsatellite diversity of crossbred horses raised in Bosnia and Herzegovina. Genomic DNA was extracted from blood samples of 20 individuals (KBA group – 7 individuals, crosses between Bosnian and Herzegovinian mountain horse and Arabian horse; KBR group – 9 individuals...
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| Format: | Article |
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University of Sarajevo, Institute for Genetic Engineering and Biotechnology
2017-06-01
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| Series: | Genetics & Applications |
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| Online Access: | https://genapp.ba/editions/index.php/journal/article/view/107 |
| _version_ | 1828169368505155584 |
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| author | Dunja Rukavina Danica Hasanbašić Belma Kalamujić Stroil Naris Pojskić |
| author_facet | Dunja Rukavina Danica Hasanbašić Belma Kalamujić Stroil Naris Pojskić |
| author_sort | Dunja Rukavina |
| collection | DOAJ |
| description |
The focus of this study was microsatellite diversity of crossbred horses raised in Bosnia and Herzegovina. Genomic DNA was extracted from blood samples of 20 individuals (KBA group – 7 individuals, crosses between Bosnian and Herzegovinian mountain horse and Arabian horse; KBR group – 9 individuals, crosses between Bosnian and Herzegovinian mountain and Belgian horses, crosses between Bosnian and Herzegovinian mountain horses and Holstein, crosses between Bosnian and Herzegovinian mountain and Lipizzaner horses and KBN group – 4 individuals, crosses between Bosnian and Herzegovinian mountain horse with an unknown origin of the other parent). The samples were profiled using 17 microsatellite markers. This method consisted of multiplex PCR procedure and generated reasonable amplification across all the loci. All samples were genotyped successfully. Considering all the observed parameters, VHL20 locus showed the highest microsatellite diversity. Locus HMS7 was the least variable in KBR group, while HMS1 locus was the least diverse in KBN group. The highest microsatellite diversity in KBA group was found at AHT5 locus while HTG6 locus was the least diverse. Obtained results suggest that the investigated populations of crossbred horses from Bosnia and Herzegovina are not affected by substantial loss of genetic diversity, as indicated by the presence of reasonably high level of genetic variation. An increase in the inbreeding coefficient and sufficient heterogenity in KBN group indicate occurrence of consanguineous mating. The present research contributes to the knowledge of current status of genetic structure of the investigated crossbred horses.
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| first_indexed | 2024-04-12T02:50:22Z |
| format | Article |
| id | doaj.art-0ad5cada8a954bd9bbad55e938644b6f |
| institution | Directory Open Access Journal |
| issn | 2566-2937 2566-431X |
| language | English |
| last_indexed | 2024-04-12T02:50:22Z |
| publishDate | 2017-06-01 |
| publisher | University of Sarajevo, Institute for Genetic Engineering and Biotechnology |
| record_format | Article |
| series | Genetics & Applications |
| spelling | doaj.art-0ad5cada8a954bd9bbad55e938644b6f2022-12-22T03:51:00ZengUniversity of Sarajevo, Institute for Genetic Engineering and BiotechnologyGenetics & Applications2566-29372566-431X2017-06-0111MICROSATELLITE DIVERSITY OF CROSSBRED HORSES RAISED IN BOSNIA AND HERZEGOVINADunja Rukavina0Danica Hasanbašić1Belma Kalamujić Stroil2Naris Pojskić3University of Sarajevo, Veterinary Faculty, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo, Veterinary Faculty, Sarajevo, Bosnia and HerzegovinaUniversity of Sarajevo, Institute for Genetic Engineering and Biotechnology (INGEB), Sarajevo, B&HUniversity of Sarajevo, Institute for Genetic Engineering and Biotechnology (INGEB), Sarajevo, B&H The focus of this study was microsatellite diversity of crossbred horses raised in Bosnia and Herzegovina. Genomic DNA was extracted from blood samples of 20 individuals (KBA group – 7 individuals, crosses between Bosnian and Herzegovinian mountain horse and Arabian horse; KBR group – 9 individuals, crosses between Bosnian and Herzegovinian mountain and Belgian horses, crosses between Bosnian and Herzegovinian mountain horses and Holstein, crosses between Bosnian and Herzegovinian mountain and Lipizzaner horses and KBN group – 4 individuals, crosses between Bosnian and Herzegovinian mountain horse with an unknown origin of the other parent). The samples were profiled using 17 microsatellite markers. This method consisted of multiplex PCR procedure and generated reasonable amplification across all the loci. All samples were genotyped successfully. Considering all the observed parameters, VHL20 locus showed the highest microsatellite diversity. Locus HMS7 was the least variable in KBR group, while HMS1 locus was the least diverse in KBN group. The highest microsatellite diversity in KBA group was found at AHT5 locus while HTG6 locus was the least diverse. Obtained results suggest that the investigated populations of crossbred horses from Bosnia and Herzegovina are not affected by substantial loss of genetic diversity, as indicated by the presence of reasonably high level of genetic variation. An increase in the inbreeding coefficient and sufficient heterogenity in KBN group indicate occurrence of consanguineous mating. The present research contributes to the knowledge of current status of genetic structure of the investigated crossbred horses. https://genapp.ba/editions/index.php/journal/article/view/107crossbred horsegenetic diversitymolecular markers |
| spellingShingle | Dunja Rukavina Danica Hasanbašić Belma Kalamujić Stroil Naris Pojskić MICROSATELLITE DIVERSITY OF CROSSBRED HORSES RAISED IN BOSNIA AND HERZEGOVINA Genetics & Applications crossbred horse genetic diversity molecular markers |
| title | MICROSATELLITE DIVERSITY OF CROSSBRED HORSES RAISED IN BOSNIA AND HERZEGOVINA |
| title_full | MICROSATELLITE DIVERSITY OF CROSSBRED HORSES RAISED IN BOSNIA AND HERZEGOVINA |
| title_fullStr | MICROSATELLITE DIVERSITY OF CROSSBRED HORSES RAISED IN BOSNIA AND HERZEGOVINA |
| title_full_unstemmed | MICROSATELLITE DIVERSITY OF CROSSBRED HORSES RAISED IN BOSNIA AND HERZEGOVINA |
| title_short | MICROSATELLITE DIVERSITY OF CROSSBRED HORSES RAISED IN BOSNIA AND HERZEGOVINA |
| title_sort | microsatellite diversity of crossbred horses raised in bosnia and herzegovina |
| topic | crossbred horse genetic diversity molecular markers |
| url | https://genapp.ba/editions/index.php/journal/article/view/107 |
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