Effect of Brain-derived Neurotrophic Factor (BDNF) in Organotypic Retinal Cultures
ABSTRACT Purpose To study the influence of recombinant brain-derived neurotrophic factor (BDNF) on organotypic retinal cultures. Material and methods Experiments were performed in human and rat retinal explants cultured in culture dishes, flasks and flasks for roller cultivation. BDNF was...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
| Published: |
Publishing house "Ophthalmology"
2009-02-01
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| Series: | Офтальмохирургия |
| Online Access: | http://www.eyepress.ru/article.aspx?8162 |
| _version_ | 1797946612509573120 |
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| author | N.A. Gavrilova N.I. Lanevskaya L.M. Bakaeva S.A. Borzenok A.V. Shatskikh I.N. Saburina S.A. Sergeev G.V. Pavlova E.Yu. Rybalkina A.V. Revishchin |
| author_facet | N.A. Gavrilova N.I. Lanevskaya L.M. Bakaeva S.A. Borzenok A.V. Shatskikh I.N. Saburina S.A. Sergeev G.V. Pavlova E.Yu. Rybalkina A.V. Revishchin |
| author_sort | N.A. Gavrilova |
| collection | DOAJ |
| description | ABSTRACT
Purpose
To study the influence of recombinant brain-derived neurotrophic factor (BDNF) on organotypic retinal cultures.
Material and methods
Experiments were performed in human and rat retinal explants cultured in culture dishes, flasks and flasks for roller cultivation. BDNF was added at the concentration of 100 ng⁄ml. Cultures were tested for viability and stained immunohistochemically for neuronal markers. Culture conditions and results of cultivation were controlled using phase contrast and fluorescent microscopes.
Conclusions
Results of the study showed that cultivation of organotypic cultures of the human and rat retina in the presence of BDNF at the concentration of 100 ng⁄ml increases viability of retinal cells. Active cell migration and outgrowth of β-III-tubulin-positive axon-like processes of neuronal origin outside the borders of explants were observed. |
| first_indexed | 2024-04-10T21:13:48Z |
| format | Article |
| id | doaj.art-0ae0c21ac40c4561af9f28419c3adbea |
| institution | Directory Open Access Journal |
| issn | 0235-4160 0235-4160 |
| language | Russian |
| last_indexed | 2024-04-10T21:13:48Z |
| publishDate | 2009-02-01 |
| publisher | Publishing house "Ophthalmology" |
| record_format | Article |
| series | Офтальмохирургия |
| spelling | doaj.art-0ae0c21ac40c4561af9f28419c3adbea2023-01-20T12:34:52ZrusPublishing house "Ophthalmology"Офтальмохирургия0235-41600235-41602009-02-0114447Effect of Brain-derived Neurotrophic Factor (BDNF) in Organotypic Retinal CulturesN.A. Gavrilova0N.I. Lanevskaya1L.M. Bakaeva2S.A. Borzenok3 A.V. Shatskikh4I.N. Saburina5S.A. Sergeev6G.V. Pavlova7E.Yu. Rybalkina8A.V. Revishchin9Moscow State Medico-stomatological University Moscow State Medico-stomatological University Moscow State Medico-stomatological University S.N. Fyodoroc Eye Microsurgery Complex S.N. Fyodoroc Eye Microsurgery Complex Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences Research Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences Institute of Gene Biology, Russian Academy of Sciences Institute of Gene Biology, Russian Academy of Sciences Institute of Gene Biology, Russian Academy of SciencesABSTRACT Purpose To study the influence of recombinant brain-derived neurotrophic factor (BDNF) on organotypic retinal cultures. Material and methods Experiments were performed in human and rat retinal explants cultured in culture dishes, flasks and flasks for roller cultivation. BDNF was added at the concentration of 100 ng⁄ml. Cultures were tested for viability and stained immunohistochemically for neuronal markers. Culture conditions and results of cultivation were controlled using phase contrast and fluorescent microscopes. Conclusions Results of the study showed that cultivation of organotypic cultures of the human and rat retina in the presence of BDNF at the concentration of 100 ng⁄ml increases viability of retinal cells. Active cell migration and outgrowth of β-III-tubulin-positive axon-like processes of neuronal origin outside the borders of explants were observed.http://www.eyepress.ru/article.aspx?8162 |
| spellingShingle | N.A. Gavrilova N.I. Lanevskaya L.M. Bakaeva S.A. Borzenok A.V. Shatskikh I.N. Saburina S.A. Sergeev G.V. Pavlova E.Yu. Rybalkina A.V. Revishchin Effect of Brain-derived Neurotrophic Factor (BDNF) in Organotypic Retinal Cultures Офтальмохирургия |
| title | Effect of Brain-derived Neurotrophic Factor (BDNF) in Organotypic Retinal Cultures |
| title_full | Effect of Brain-derived Neurotrophic Factor (BDNF) in Organotypic Retinal Cultures |
| title_fullStr | Effect of Brain-derived Neurotrophic Factor (BDNF) in Organotypic Retinal Cultures |
| title_full_unstemmed | Effect of Brain-derived Neurotrophic Factor (BDNF) in Organotypic Retinal Cultures |
| title_short | Effect of Brain-derived Neurotrophic Factor (BDNF) in Organotypic Retinal Cultures |
| title_sort | effect of brain derived neurotrophic factor bdnf in organotypic retinal cultures |
| url | http://www.eyepress.ru/article.aspx?8162 |
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