Investigating the causal impact of gut microbiota on glioblastoma: a bidirectional Mendelian randomization study
Abstract Background Currently, the influence of microbiota on the occurrence, progression, and treatment of cancer is a topic of considerable research interest. Therefore, based on the theory of the gut-brain axis proved by previous studies, our objective was to uncover the causal relationship betwe...
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09885-2 |
| _version_ | 1797377178293239808 |
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| author | Chuan Zeng Chaolong Zhang Chunming He Haimin Song |
| author_facet | Chuan Zeng Chaolong Zhang Chunming He Haimin Song |
| author_sort | Chuan Zeng |
| collection | DOAJ |
| description | Abstract Background Currently, the influence of microbiota on the occurrence, progression, and treatment of cancer is a topic of considerable research interest. Therefore, based on the theory of the gut-brain axis proved by previous studies, our objective was to uncover the causal relationship between glioblastoma and the gut microbiome using Mendelian randomization analysis. Methods We conducted a bidirectional Mendelian randomization study using summary statistics of gut microbiota derived from the MiBioGen consortium, the largest database of gut microbiota. Summary statistics for glioblastoma were obtained from IEU OpenGWAS project, which included 91 cases and 218,701 controls. We assessed the presence of heterogeneity and horizontal pleiotropy in the analyzed data. We primarily employed the inverse variance weighting method to investigate the causal relationship between gut microbiota and glioblastoma after excluding cases of horizontal pleiotropy. Four other analysis methods were employed as supplementary. Excluding abnormal results based on leave-one-out sensitivity analysis. Finally, reverse Mendelian randomization analysis was performed. Results Four genus-level taxa and one family-level taxa exhibited causal associations with glioblastoma. And these results of reverse Mendelian randomization analysis shown glioblastoma exhibited causal associations with three genus-level taxa and one family-level taxa. However, the Prevotella7(Forward, P=0.006, OR=0.34, 95%CI:0.158-0.732; Reverse, P=0.004, OR=0.972, 95%CI:0.953-0.991) shown the causal associations with glioblastoma in the bidirectional Mendelian randomization. Conclusions In this bidirectional Mendelian randomization study, we identified five gut microbiota species with causal associations to glioblastoma. However, additional randomized controlled trials are required to clarify the impact of gut microbiota on glioblastoma and to reveal its precise mechanisms. |
| first_indexed | 2024-03-08T19:48:59Z |
| format | Article |
| id | doaj.art-0ae18c09eb5e4aa39d5e433842f2c9db |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-08T19:48:59Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-0ae18c09eb5e4aa39d5e433842f2c9db2023-12-24T12:10:27ZengBMCBMC Genomics1471-21642023-12-0124111710.1186/s12864-023-09885-2Investigating the causal impact of gut microbiota on glioblastoma: a bidirectional Mendelian randomization studyChuan Zeng0Chaolong Zhang1Chunming He2Haimin Song3The First Clinical Medical College of Gannan Medical UniversityThe First Clinical Medical College of Gannan Medical UniversityDepartment of Neurosurgery, First Affiliated Hospital of Gannan Medical UniversityDepartment of Neurosurgery, First Affiliated Hospital of Gannan Medical UniversityAbstract Background Currently, the influence of microbiota on the occurrence, progression, and treatment of cancer is a topic of considerable research interest. Therefore, based on the theory of the gut-brain axis proved by previous studies, our objective was to uncover the causal relationship between glioblastoma and the gut microbiome using Mendelian randomization analysis. Methods We conducted a bidirectional Mendelian randomization study using summary statistics of gut microbiota derived from the MiBioGen consortium, the largest database of gut microbiota. Summary statistics for glioblastoma were obtained from IEU OpenGWAS project, which included 91 cases and 218,701 controls. We assessed the presence of heterogeneity and horizontal pleiotropy in the analyzed data. We primarily employed the inverse variance weighting method to investigate the causal relationship between gut microbiota and glioblastoma after excluding cases of horizontal pleiotropy. Four other analysis methods were employed as supplementary. Excluding abnormal results based on leave-one-out sensitivity analysis. Finally, reverse Mendelian randomization analysis was performed. Results Four genus-level taxa and one family-level taxa exhibited causal associations with glioblastoma. And these results of reverse Mendelian randomization analysis shown glioblastoma exhibited causal associations with three genus-level taxa and one family-level taxa. However, the Prevotella7(Forward, P=0.006, OR=0.34, 95%CI:0.158-0.732; Reverse, P=0.004, OR=0.972, 95%CI:0.953-0.991) shown the causal associations with glioblastoma in the bidirectional Mendelian randomization. Conclusions In this bidirectional Mendelian randomization study, we identified five gut microbiota species with causal associations to glioblastoma. However, additional randomized controlled trials are required to clarify the impact of gut microbiota on glioblastoma and to reveal its precise mechanisms.https://doi.org/10.1186/s12864-023-09885-2GlioblastomaGut microbiotaMendelian randomizationCausalityGut-brain axis |
| spellingShingle | Chuan Zeng Chaolong Zhang Chunming He Haimin Song Investigating the causal impact of gut microbiota on glioblastoma: a bidirectional Mendelian randomization study BMC Genomics Glioblastoma Gut microbiota Mendelian randomization Causality Gut-brain axis |
| title | Investigating the causal impact of gut microbiota on glioblastoma: a bidirectional Mendelian randomization study |
| title_full | Investigating the causal impact of gut microbiota on glioblastoma: a bidirectional Mendelian randomization study |
| title_fullStr | Investigating the causal impact of gut microbiota on glioblastoma: a bidirectional Mendelian randomization study |
| title_full_unstemmed | Investigating the causal impact of gut microbiota on glioblastoma: a bidirectional Mendelian randomization study |
| title_short | Investigating the causal impact of gut microbiota on glioblastoma: a bidirectional Mendelian randomization study |
| title_sort | investigating the causal impact of gut microbiota on glioblastoma a bidirectional mendelian randomization study |
| topic | Glioblastoma Gut microbiota Mendelian randomization Causality Gut-brain axis |
| url | https://doi.org/10.1186/s12864-023-09885-2 |
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