Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction
Abstract Background Acute myocardial infarction (AMI) is characterized by an inflammatory process in which T cell plays a key role. However, the profile of immune microenvironment in AMI is still uncertain. High-throughput sequencing of T cell receptor (TCR) provides deep insight into monitoring the...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2019-02-01
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| Series: | Journal of Translational Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12967-019-1788-4 |
| _version_ | 1818457817212780544 |
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| author | Dan Li Longgang Hu Qing Liang Cuijuan Zhang Yunzhen Shi Bin Wang Kejia Wang |
| author_facet | Dan Li Longgang Hu Qing Liang Cuijuan Zhang Yunzhen Shi Bin Wang Kejia Wang |
| author_sort | Dan Li |
| collection | DOAJ |
| description | Abstract Background Acute myocardial infarction (AMI) is characterized by an inflammatory process in which T cell plays a key role. However, the profile of immune microenvironment in AMI is still uncertain. High-throughput sequencing of T cell receptor (TCR) provides deep insight into monitoring the immune microenvironment. Methods 30 patients with AMI were enrolled and 30 healthy individuals were recruited as controls. Flow cytometer were used to analyze the distribution of αβ T cells and their CD69 expression from peripheral leukomonocytes. TCRβ repertoire library was amplified by two-round multiplex PCR and detected by next-generation sequencing (NGS). Results The percentage of αβ T cells in AMI patients were significantly restricted than those in healthy controls, while the highly activated αβ T cells along with distinguishing usage of variable (V), diversity (D) and joining (J) gene segments were also found in AMI patients. In addition, AMI induced a significantly restricted CDR3 amino acid (AA) diversity and remarkably reconstituted TCR immune repertoires. Finally, we identified several AMI-associated tendency of CDR3 AAs expression after AMI. Conclusions Our work suggests that the aberrant αβ T cells distribution and activation may associated with the pathogenesis of AMI and demonstrates a reconstitution of TCRβ immune repertoire after AMI. |
| first_indexed | 2024-12-14T22:48:35Z |
| format | Article |
| id | doaj.art-0ae843d12baf44a29bfbb0a8d3d5b20d |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-14T22:48:35Z |
| publishDate | 2019-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-0ae843d12baf44a29bfbb0a8d3d5b20d2022-12-21T22:44:46ZengBMCJournal of Translational Medicine1479-58762019-02-011711910.1186/s12967-019-1788-4Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarctionDan Li0Longgang Hu1Qing Liang2Cuijuan Zhang3Yunzhen Shi4Bin Wang5Kejia Wang6Department of Cardiology, The Affiliated Hospital of Qingdao UniversityDepartment of Cardiovascular Medicine, The Affiliated Cardiovascular Hospital of Qingdao UniversityCollege of Basic Medicine, Qingdao UniversityDepartment of Cardiology, The Affiliated Hospital of Qingdao UniversityCenter of Patients, West China Second University Hospital, Sichuan UniversityCollege of Basic Medicine, Qingdao UniversityCollege of Basic Medicine, Qingdao UniversityAbstract Background Acute myocardial infarction (AMI) is characterized by an inflammatory process in which T cell plays a key role. However, the profile of immune microenvironment in AMI is still uncertain. High-throughput sequencing of T cell receptor (TCR) provides deep insight into monitoring the immune microenvironment. Methods 30 patients with AMI were enrolled and 30 healthy individuals were recruited as controls. Flow cytometer were used to analyze the distribution of αβ T cells and their CD69 expression from peripheral leukomonocytes. TCRβ repertoire library was amplified by two-round multiplex PCR and detected by next-generation sequencing (NGS). Results The percentage of αβ T cells in AMI patients were significantly restricted than those in healthy controls, while the highly activated αβ T cells along with distinguishing usage of variable (V), diversity (D) and joining (J) gene segments were also found in AMI patients. In addition, AMI induced a significantly restricted CDR3 amino acid (AA) diversity and remarkably reconstituted TCR immune repertoires. Finally, we identified several AMI-associated tendency of CDR3 AAs expression after AMI. Conclusions Our work suggests that the aberrant αβ T cells distribution and activation may associated with the pathogenesis of AMI and demonstrates a reconstitution of TCRβ immune repertoire after AMI.http://link.springer.com/article/10.1186/s12967-019-1788-4T cell receptor betaImmune repertoireAcute myocardial infarctionNext-generation sequencing |
| spellingShingle | Dan Li Longgang Hu Qing Liang Cuijuan Zhang Yunzhen Shi Bin Wang Kejia Wang Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction Journal of Translational Medicine T cell receptor beta Immune repertoire Acute myocardial infarction Next-generation sequencing |
| title | Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction |
| title_full | Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction |
| title_fullStr | Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction |
| title_full_unstemmed | Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction |
| title_short | Peripheral T cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction |
| title_sort | peripheral t cell receptor beta immune repertoire is promptly reconstituted after acute myocardial infarction |
| topic | T cell receptor beta Immune repertoire Acute myocardial infarction Next-generation sequencing |
| url | http://link.springer.com/article/10.1186/s12967-019-1788-4 |
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