Autologous platelet rich plasma have positive eff ect on ram spermatozoa during cryopreservation in non-breeding season
The aim of this study was to evaluate the eff ects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender on ram semen after thawing. Semen samples were collected from nine Kıvırcık rams and each semen was divided into four equal aliquots and mixed with diff erent concentratio...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2022-02-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2886 |
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| author | Selim ALCAY Ahmet AKTAR Davut KOCA Mehmet Ali KILIC Mustafa AKKASOGLU Mehmet Melih YILMAZ Hakan SAGIRKAYA |
| author_facet | Selim ALCAY Ahmet AKTAR Davut KOCA Mehmet Ali KILIC Mustafa AKKASOGLU Mehmet Melih YILMAZ Hakan SAGIRKAYA |
| author_sort | Selim ALCAY |
| collection | DOAJ |
| description | The aim of this study was to evaluate the eff ects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender on ram semen
after thawing. Semen samples were collected from nine Kıvırcık rams and each semen was divided into four equal aliquots and mixed with
diff erent concentrations of PRP supported extenders [no PRP (control), 12.5x106/mL PRP, 25x106/mL PRP, or 50x106/mL PRP)]. Motility, plasma
membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations
(MDA) were measured and analyzed after thawing. The results showed that 25x106/mL PRP group had positive eff ect on motility (61.67±3.81),
membrane functional integrity (71.00±2.96) and MDA levels (5.13±0.64) at post-thawed (P<0.05). It was determined that 25x106/mL PRP and
50x106/mL PRP groups were more eff ective than other groups in terms of mitochondrial membrane potential (69.50±1.93), acrosome integrity
(78.04±2.65) and DNA integrity (5.33±0.92). The results of the study showed that autologous PRP has a protective eff ect in the cryopreservation
of ram spermatozoa. |
| first_indexed | 2024-03-13T04:38:57Z |
| format | Article |
| id | doaj.art-0ae9c3396b8e4ba4bb55538838866f8c |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:57Z |
| publishDate | 2022-02-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-0ae9c3396b8e4ba4bb55538838866f8c2023-06-19T06:28:21ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512022-02-0128222923410.9775/kvfd.2021.267632886Autologous platelet rich plasma have positive eff ect on ram spermatozoa during cryopreservation in non-breeding seasonSelim ALCAY0Ahmet AKTAR1Davut KOCA2Mehmet Ali KILIC3Mustafa AKKASOGLU4Mehmet Melih YILMAZ5Hakan SAGIRKAYA6Bursa Uludag University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-16059 Gorukle, Bursa - TÜRKİYEBursa Uludag University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-16059 Gorukle, Bursa - TÜRKİYEBursa Uludag University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, TR-16059 Gorukle, Bursa - TÜRKİYEBursa Uludag University, Faculty of Veterinary Medicine, Department of Obstetrics and Gynecology, TR-16059 Gorukle, Bursa - TÜRKİYEBursa Uludag University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-16059 Gorukle, Bursa - TÜRKİYEBursa Uludag University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-16059 Gorukle, Bursa - TÜRKİYEBursa Uludag University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, TR-16059 Gorukle, Bursa - TÜRKİYEThe aim of this study was to evaluate the eff ects of autologous platelet-rich plasma (PRP) supplemented egg yolk based extender on ram semen after thawing. Semen samples were collected from nine Kıvırcık rams and each semen was divided into four equal aliquots and mixed with diff erent concentrations of PRP supported extenders [no PRP (control), 12.5x106/mL PRP, 25x106/mL PRP, or 50x106/mL PRP)]. Motility, plasma membrane functional integrity, acrosome integrity, mitochondrial membrane potential, DNA integrity and malondialdehyde concentrations (MDA) were measured and analyzed after thawing. The results showed that 25x106/mL PRP group had positive eff ect on motility (61.67±3.81), membrane functional integrity (71.00±2.96) and MDA levels (5.13±0.64) at post-thawed (P<0.05). It was determined that 25x106/mL PRP and 50x106/mL PRP groups were more eff ective than other groups in terms of mitochondrial membrane potential (69.50±1.93), acrosome integrity (78.04±2.65) and DNA integrity (5.33±0.92). The results of the study showed that autologous PRP has a protective eff ect in the cryopreservation of ram spermatozoa.https://vetdergikafkas.org/pdf.php?id=2886ram semenplatelet-rich plasmacryopreservationpost-thawed |
| spellingShingle | Selim ALCAY Ahmet AKTAR Davut KOCA Mehmet Ali KILIC Mustafa AKKASOGLU Mehmet Melih YILMAZ Hakan SAGIRKAYA Autologous platelet rich plasma have positive eff ect on ram spermatozoa during cryopreservation in non-breeding season Kafkas Universitesi Veteriner Fakültesi Dergisi ram semen platelet-rich plasma cryopreservation post-thawed |
| title | Autologous platelet rich plasma have positive eff ect on ram spermatozoa during cryopreservation in non-breeding season |
| title_full | Autologous platelet rich plasma have positive eff ect on ram spermatozoa during cryopreservation in non-breeding season |
| title_fullStr | Autologous platelet rich plasma have positive eff ect on ram spermatozoa during cryopreservation in non-breeding season |
| title_full_unstemmed | Autologous platelet rich plasma have positive eff ect on ram spermatozoa during cryopreservation in non-breeding season |
| title_short | Autologous platelet rich plasma have positive eff ect on ram spermatozoa during cryopreservation in non-breeding season |
| title_sort | autologous platelet rich plasma have positive eff ect on ram spermatozoa during cryopreservation in non breeding season |
| topic | ram semen platelet-rich plasma cryopreservation post-thawed |
| url | https://vetdergikafkas.org/pdf.php?id=2886 |
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