IL17A and IL17F genes polymorphisms are associated with histopathological changes in transplanted kidney
Abstract Background Interleukin 17 is a proinflammatory cytokine involved in immune response after allograft transplantation. IL-17 family of proinflammatory cytokines includes IL-17A and IL-17F. Previous studies have demonstrated that the rs2275913 IL17A and the rs11465553 IL17F gene polymorphism a...
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| Format: | Article |
| Language: | English |
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BMC
2019-04-01
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| Series: | BMC Nephrology |
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| Online Access: | http://link.springer.com/article/10.1186/s12882-019-1308-z |
| _version_ | 1819162158080983040 |
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| author | Leszek Domanski Karolina Kłoda Maciej Patrzyk Magda Wisniewska Krzysztof Safranow Jerzy Sienko Tadeusz Sulikowski Marzena Staniszewska Andrzej Pawlik |
| author_facet | Leszek Domanski Karolina Kłoda Maciej Patrzyk Magda Wisniewska Krzysztof Safranow Jerzy Sienko Tadeusz Sulikowski Marzena Staniszewska Andrzej Pawlik |
| author_sort | Leszek Domanski |
| collection | DOAJ |
| description | Abstract Background Interleukin 17 is a proinflammatory cytokine involved in immune response after allograft transplantation. IL-17 family of proinflammatory cytokines includes IL-17A and IL-17F. Previous studies have demonstrated that the rs2275913 IL17A and the rs11465553 IL17F gene polymorphism are associated with kidney allograft function. Because of the association between these polymorphisms and post-transplant immune response, we assume that these single nucleotide polymorphisms may affect morphological structure of transplanted kidney. The aim of this study was to examine the association of rs2275913 IL17A and rs2397084, rs11465553 and rs763780 IL17F gene polymorphisms with histopathological changes in transplanted kidney biopsies such as: glomerulitis, tubulitis, arteritis, cell infilitration and fibrosis. Methods The study enrolled 82 patients after renal graft transplantation in whom a kidney biopsy was performed because of impaired graft function. The rs2397084 T > C (Glu126Gly), rs11465553 G > A (Val155Ile) and rs763780 T > C (His167Arg) polymorphisms within the IL17F gene and the rs2275913 A > G (− 197 A > G) polymorphism within the IL17A gene promoter were genotyped using TaqMan genotyping assays on a 7500 FAST Real-Time PCR System (Applied Biosystems, USA). Results There was a significant association between the rs2275913 IL17A gene polymorphism and the grade of tubulitis, which was more severe among patients with the A allele, compared to recipients with the GG genotype (GG vs. AG + AA, P = 0.02), and with the grade of arteriolar hyaline thickening and mesangial matrix increase, which were more severe among patients with the G allele compared to recipients with the AA genotype (AA vs. AG + GG, P = 0.01 and P = 0.04, respectively). Tubular atrophy and interstitial fibrosis were more severe among individuals with the C allele at the rs763780 IL17F gene polymorphism (TT vs. TC, P = 0.09 and P = 0.017, respectively). However, it should be taken into account that the statistical significance was achieved without correction for multiple testing, and no significant association would remain significant after such correction. Conclusions The results of this study may suggest a possible association between the rs2275913 IL17A and rs2275913 IL17A gene polymorphisms and some histopathological changes in transplanted kidney biopsies. |
| first_indexed | 2024-12-22T17:23:47Z |
| format | Article |
| id | doaj.art-0aea9f394eae4703a033eb33064426f8 |
| institution | Directory Open Access Journal |
| issn | 1471-2369 |
| language | English |
| last_indexed | 2024-12-22T17:23:47Z |
| publishDate | 2019-04-01 |
| publisher | BMC |
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| series | BMC Nephrology |
| spelling | doaj.art-0aea9f394eae4703a033eb33064426f82022-12-21T18:18:46ZengBMCBMC Nephrology1471-23692019-04-012011910.1186/s12882-019-1308-zIL17A and IL17F genes polymorphisms are associated with histopathological changes in transplanted kidneyLeszek Domanski0Karolina Kłoda1Maciej Patrzyk2Magda Wisniewska3Krzysztof Safranow4Jerzy Sienko5Tadeusz Sulikowski6Marzena Staniszewska7Andrzej Pawlik8Department of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University in SzczecinDepartment of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University in SzczecinDepartment of Surgery, University Medical Center GreifswaldDepartment of Nephrology, Transplantology and Internal Medicine, Pomeranian Medical University in SzczecinDepartment of Biochemistry and Medical Chemistry, Pomeranian Medical University in SzczecinDepartment of Surgery, Pomeranian Medical University in SzczecinDepartment of Surgery, Pomeranian Medical University in SzczecinDepartment of Physiology, Pomeranian Medical University in SzczecinDepartment of Physiology, Pomeranian Medical University in SzczecinAbstract Background Interleukin 17 is a proinflammatory cytokine involved in immune response after allograft transplantation. IL-17 family of proinflammatory cytokines includes IL-17A and IL-17F. Previous studies have demonstrated that the rs2275913 IL17A and the rs11465553 IL17F gene polymorphism are associated with kidney allograft function. Because of the association between these polymorphisms and post-transplant immune response, we assume that these single nucleotide polymorphisms may affect morphological structure of transplanted kidney. The aim of this study was to examine the association of rs2275913 IL17A and rs2397084, rs11465553 and rs763780 IL17F gene polymorphisms with histopathological changes in transplanted kidney biopsies such as: glomerulitis, tubulitis, arteritis, cell infilitration and fibrosis. Methods The study enrolled 82 patients after renal graft transplantation in whom a kidney biopsy was performed because of impaired graft function. The rs2397084 T > C (Glu126Gly), rs11465553 G > A (Val155Ile) and rs763780 T > C (His167Arg) polymorphisms within the IL17F gene and the rs2275913 A > G (− 197 A > G) polymorphism within the IL17A gene promoter were genotyped using TaqMan genotyping assays on a 7500 FAST Real-Time PCR System (Applied Biosystems, USA). Results There was a significant association between the rs2275913 IL17A gene polymorphism and the grade of tubulitis, which was more severe among patients with the A allele, compared to recipients with the GG genotype (GG vs. AG + AA, P = 0.02), and with the grade of arteriolar hyaline thickening and mesangial matrix increase, which were more severe among patients with the G allele compared to recipients with the AA genotype (AA vs. AG + GG, P = 0.01 and P = 0.04, respectively). Tubular atrophy and interstitial fibrosis were more severe among individuals with the C allele at the rs763780 IL17F gene polymorphism (TT vs. TC, P = 0.09 and P = 0.017, respectively). However, it should be taken into account that the statistical significance was achieved without correction for multiple testing, and no significant association would remain significant after such correction. Conclusions The results of this study may suggest a possible association between the rs2275913 IL17A and rs2275913 IL17A gene polymorphisms and some histopathological changes in transplanted kidney biopsies.http://link.springer.com/article/10.1186/s12882-019-1308-zCytokinesHistopathologyIL-17Kidney allograftPolymorphism |
| spellingShingle | Leszek Domanski Karolina Kłoda Maciej Patrzyk Magda Wisniewska Krzysztof Safranow Jerzy Sienko Tadeusz Sulikowski Marzena Staniszewska Andrzej Pawlik IL17A and IL17F genes polymorphisms are associated with histopathological changes in transplanted kidney BMC Nephrology Cytokines Histopathology IL-17 Kidney allograft Polymorphism |
| title | IL17A and IL17F genes polymorphisms are associated with histopathological changes in transplanted kidney |
| title_full | IL17A and IL17F genes polymorphisms are associated with histopathological changes in transplanted kidney |
| title_fullStr | IL17A and IL17F genes polymorphisms are associated with histopathological changes in transplanted kidney |
| title_full_unstemmed | IL17A and IL17F genes polymorphisms are associated with histopathological changes in transplanted kidney |
| title_short | IL17A and IL17F genes polymorphisms are associated with histopathological changes in transplanted kidney |
| title_sort | il17a and il17f genes polymorphisms are associated with histopathological changes in transplanted kidney |
| topic | Cytokines Histopathology IL-17 Kidney allograft Polymorphism |
| url | http://link.springer.com/article/10.1186/s12882-019-1308-z |
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