Expression of MiR-148b-3p in Lung Adenocarcinoma and Its Correlation with Prognosis
Background and objective MiR-148b-3p is an important microRNA that has been reported to be significantly related to various types of cancer, but its role in lung adenocarcinoma remains elusive. The purpose of this study is to detect the expression level of miR-148b-3p in lung adenocarcinoma specimen...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Chinese Anti-Cancer Association; Chinese Antituberculosis Association
2019-05-01
|
| Series: | Chinese Journal of Lung Cancer |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.3779/j.issn.1009-3419.2019.05.07 |
| _version_ | 1819162224585867264 |
|---|---|
| author | Zekai HUANG Shaolei LI Yuanyuan MA Nan WU Yue YANG |
| author_facet | Zekai HUANG Shaolei LI Yuanyuan MA Nan WU Yue YANG |
| author_sort | Zekai HUANG |
| collection | DOAJ |
| description | Background and objective MiR-148b-3p is an important microRNA that has been reported to be significantly related to various types of cancer, but its role in lung adenocarcinoma remains elusive. The purpose of this study is to detect the expression level of miR-148b-3p in lung adenocarcinoma specimens, and to analyze its correlation with the clinicopathological features as well as the prognosis of patients with lung adenocarcinoma. Methods A total of 123 tumor specimens from lung adenocarcinoma patients who underwent surgical resection in our department from January 2011 to December 2012 were collected. The expression of miR-148b-3p was detected by quantitative real-time PCR (qRT-PCR), and its correlation with clinicopathological features of patients with lung adenocarcinoma was analyzed. Multivariate Cox proportional hazard models were used to analyze independent predictors of overall survival in patients with lung adenocarcinoma. The overall survival (OS) of patients in miR-148b-3p high expression group and miR-148b-3p low expression group were estimated by means of the Kaplan-Meier method and were compared using the Log-rank test method. Results Of the 123 patients with lung adenocarcinoma, 71 were in miR-148b-3p high expression group and 52 in low expression group. MiR-148b-3p was significantly associated with tumor grade (P=0.001) and tumor size (P=0.007), but not with age, gender, smoking history, history of alcohol, tumor thrombus, pleural invasion, node status or metastasis status. Multivariate Cox proportional hazard model analysis showed that tumor size (P=0.032), node status (P=0.005) and miR-148b-3p expression level (P=0.047) were significant independent predictors of overall survival of patients with lung adenocarcinoma. Kaplan-Meier survival analysis showed that the overall survival of patients with high expression of miR-148b-3p was significantly better than that of patients with low expression (P=0.010). Conclusion MiR-148b-3p was significantly associated with tumor grade and tumor size in lung adenocarcinoma, and served as an independent predictor of overall survival of patients with lung adenocarcinoma. The overall survival of patients with high expression level of miR-148b-3p was significantly better than that of patients with low expression. |
| first_indexed | 2024-12-22T17:24:50Z |
| format | Article |
| id | doaj.art-0aed47437f264b83a3531a978130d9d6 |
| institution | Directory Open Access Journal |
| issn | 1009-3419 1999-6187 |
| language | zho |
| last_indexed | 2024-12-22T17:24:50Z |
| publishDate | 2019-05-01 |
| publisher | Chinese Anti-Cancer Association; Chinese Antituberculosis Association |
| record_format | Article |
| series | Chinese Journal of Lung Cancer |
| spelling | doaj.art-0aed47437f264b83a3531a978130d9d62022-12-21T18:18:44ZzhoChinese Anti-Cancer Association; Chinese Antituberculosis AssociationChinese Journal of Lung Cancer1009-34191999-61872019-05-0122530631110.3779/j.issn.1009-3419.2019.05.07Expression of MiR-148b-3p in Lung Adenocarcinoma and Its Correlation with PrognosisZekai HUANG0Shaolei LI1Yuanyuan MA2Nan WU3Yue YANG4Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery II, Peking University Cancer Hospital and Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery II, Peking University Cancer Hospital and Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery II, Peking University Cancer Hospital and Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery II, Peking University Cancer Hospital and Institute, Beijing 100142, ChinaKey Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Department of Thoracic Surgery II, Peking University Cancer Hospital and Institute, Beijing 100142, ChinaBackground and objective MiR-148b-3p is an important microRNA that has been reported to be significantly related to various types of cancer, but its role in lung adenocarcinoma remains elusive. The purpose of this study is to detect the expression level of miR-148b-3p in lung adenocarcinoma specimens, and to analyze its correlation with the clinicopathological features as well as the prognosis of patients with lung adenocarcinoma. Methods A total of 123 tumor specimens from lung adenocarcinoma patients who underwent surgical resection in our department from January 2011 to December 2012 were collected. The expression of miR-148b-3p was detected by quantitative real-time PCR (qRT-PCR), and its correlation with clinicopathological features of patients with lung adenocarcinoma was analyzed. Multivariate Cox proportional hazard models were used to analyze independent predictors of overall survival in patients with lung adenocarcinoma. The overall survival (OS) of patients in miR-148b-3p high expression group and miR-148b-3p low expression group were estimated by means of the Kaplan-Meier method and were compared using the Log-rank test method. Results Of the 123 patients with lung adenocarcinoma, 71 were in miR-148b-3p high expression group and 52 in low expression group. MiR-148b-3p was significantly associated with tumor grade (P=0.001) and tumor size (P=0.007), but not with age, gender, smoking history, history of alcohol, tumor thrombus, pleural invasion, node status or metastasis status. Multivariate Cox proportional hazard model analysis showed that tumor size (P=0.032), node status (P=0.005) and miR-148b-3p expression level (P=0.047) were significant independent predictors of overall survival of patients with lung adenocarcinoma. Kaplan-Meier survival analysis showed that the overall survival of patients with high expression of miR-148b-3p was significantly better than that of patients with low expression (P=0.010). Conclusion MiR-148b-3p was significantly associated with tumor grade and tumor size in lung adenocarcinoma, and served as an independent predictor of overall survival of patients with lung adenocarcinoma. The overall survival of patients with high expression level of miR-148b-3p was significantly better than that of patients with low expression.http://dx.doi.org/10.3779/j.issn.1009-3419.2019.05.07miR-148b-3pLung neoplasmsPrognosis |
| spellingShingle | Zekai HUANG Shaolei LI Yuanyuan MA Nan WU Yue YANG Expression of MiR-148b-3p in Lung Adenocarcinoma and Its Correlation with Prognosis Chinese Journal of Lung Cancer miR-148b-3p Lung neoplasms Prognosis |
| title | Expression of MiR-148b-3p in Lung Adenocarcinoma and Its Correlation with Prognosis |
| title_full | Expression of MiR-148b-3p in Lung Adenocarcinoma and Its Correlation with Prognosis |
| title_fullStr | Expression of MiR-148b-3p in Lung Adenocarcinoma and Its Correlation with Prognosis |
| title_full_unstemmed | Expression of MiR-148b-3p in Lung Adenocarcinoma and Its Correlation with Prognosis |
| title_short | Expression of MiR-148b-3p in Lung Adenocarcinoma and Its Correlation with Prognosis |
| title_sort | expression of mir 148b 3p in lung adenocarcinoma and its correlation with prognosis |
| topic | miR-148b-3p Lung neoplasms Prognosis |
| url | http://dx.doi.org/10.3779/j.issn.1009-3419.2019.05.07 |
| work_keys_str_mv | AT zekaihuang expressionofmir148b3pinlungadenocarcinomaanditscorrelationwithprognosis AT shaoleili expressionofmir148b3pinlungadenocarcinomaanditscorrelationwithprognosis AT yuanyuanma expressionofmir148b3pinlungadenocarcinomaanditscorrelationwithprognosis AT nanwu expressionofmir148b3pinlungadenocarcinomaanditscorrelationwithprognosis AT yueyang expressionofmir148b3pinlungadenocarcinomaanditscorrelationwithprognosis |