Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancer
Abstract Background PD-L1 staining using long-stored paraffin sections may not be consistent with the true PD-L1 expression of patients. Therefore, it is necessary to explore the expression of PD-L1(SP142) in paraffin sections of invasive breast cancer with different storage times and the optimal st...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-12-01
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| Series: | Diagnostic Pathology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13000-023-01423-8 |
| _version_ | 1827590747629551616 |
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| author | Jiankun He Xinran Wang Lijing Cai Zhanli Jia Chang Liu Xuemei Sun Si Wu Chunyan Ding Zi Zhang Yueping Liu |
| author_facet | Jiankun He Xinran Wang Lijing Cai Zhanli Jia Chang Liu Xuemei Sun Si Wu Chunyan Ding Zi Zhang Yueping Liu |
| author_sort | Jiankun He |
| collection | DOAJ |
| description | Abstract Background PD-L1 staining using long-stored paraffin sections may not be consistent with the true PD-L1 expression of patients. Therefore, it is necessary to explore the expression of PD-L1(SP142) in paraffin sections of invasive breast cancer with different storage times and the optimal storage temperature for unstained paraffin sections. Methods The study included 71 cases of PD-L1(SP142) positive breast cancer. The unstained paraffin sections were stored at room temperature conditions (20–25 °C), 4 °C, -20 °C and − 80 °C, respectively. PD-L1 staining was performed at 1, 2, 3, 4, 8, 12 and 24 weeks of storage. PD-L1 expression was assessed with a continuity score. Results The PD-L1 antigen was gradually lost as the storage time of paraffin sections increased. The PD-L1 positivity rate was 97.18% at 1 week for the sections stored at room temperature, and decreased from 83.10 to 71.83% for the sections stored for 2 weeks to 4 weeks, and 61.97%, 54.93%, and 32.93% for 8, 12, and 24 weeks, respectively. When stored at low temperatures of 4 °C, -20 °C and − 80 °C, the positivity rate decreases with the same trend but more slowly compared to room temperature. The mean IC score of PD-L1 also showed a gradual decrease in all cases. In the consistency analysis, PD-L1 expression in slices stored at room temperature for 2 weeks was consistent with PD-L1 expression in fresh slices (ICC ≥ 0.9 for all slices), and PD-L1 expression in slices stored at 4 °C or -20 °C for 4 weeks was consistent with PD-L1 expression in fresh slices (ICC ≥ 0.9 for all slices). When stored under refrigeration at -80 °C, PD-L1 expression in slices stored for 3 weeks was consistent with that in fresh slices (ICC ≥ 0.9). Conclusions To our knowledge, this is the first article on the effect of preservation time and preservation temperature of paraffin sections on PD-L1 expression in breast cancer. Long-term storage of paraffin sections of unstained invasive breast cancer can lead to antigen loss of PD-L1 (SP142). Refrigerated storage of paraffin sections can delay antigen loss, with best results at 4 °C or -20 °C, and a storage time of no more than 4 weeks is recommended. |
| first_indexed | 2024-03-09T01:21:08Z |
| format | Article |
| id | doaj.art-0aedbf6a831b4cbf9d2369a21b2e95ec |
| institution | Directory Open Access Journal |
| issn | 1746-1596 |
| language | English |
| last_indexed | 2024-03-09T01:21:08Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Diagnostic Pathology |
| spelling | doaj.art-0aedbf6a831b4cbf9d2369a21b2e95ec2023-12-10T12:05:33ZengBMCDiagnostic Pathology1746-15962023-12-011811910.1186/s13000-023-01423-8Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancerJiankun He0Xinran Wang1Lijing Cai2Zhanli Jia3Chang Liu4Xuemei Sun5Si Wu6Chunyan Ding7Zi Zhang8Yueping Liu9Department of Pathology, the Fourth Hospital of Hebei Medical UniversityDepartment of Pathology, the Fourth Hospital of Hebei Medical UniversityDepartment of Pathology, the Fourth Hospital of Hebei Medical UniversityDepartment of Pathology, the Fourth Hospital of Hebei Medical UniversityDepartment of Pathology, the Fourth Hospital of Hebei Medical UniversityDepartment of Pathology, the Fourth Hospital of Hebei Medical UniversityDepartment of Pathology, the Fourth Hospital of Hebei Medical UniversityDepartment of Pathology, the Fourth Hospital of Hebei Medical UniversityDepartment of Pathology, the Fourth Hospital of Hebei Medical UniversityDepartment of Pathology, the Fourth Hospital of Hebei Medical UniversityAbstract Background PD-L1 staining using long-stored paraffin sections may not be consistent with the true PD-L1 expression of patients. Therefore, it is necessary to explore the expression of PD-L1(SP142) in paraffin sections of invasive breast cancer with different storage times and the optimal storage temperature for unstained paraffin sections. Methods The study included 71 cases of PD-L1(SP142) positive breast cancer. The unstained paraffin sections were stored at room temperature conditions (20–25 °C), 4 °C, -20 °C and − 80 °C, respectively. PD-L1 staining was performed at 1, 2, 3, 4, 8, 12 and 24 weeks of storage. PD-L1 expression was assessed with a continuity score. Results The PD-L1 antigen was gradually lost as the storage time of paraffin sections increased. The PD-L1 positivity rate was 97.18% at 1 week for the sections stored at room temperature, and decreased from 83.10 to 71.83% for the sections stored for 2 weeks to 4 weeks, and 61.97%, 54.93%, and 32.93% for 8, 12, and 24 weeks, respectively. When stored at low temperatures of 4 °C, -20 °C and − 80 °C, the positivity rate decreases with the same trend but more slowly compared to room temperature. The mean IC score of PD-L1 also showed a gradual decrease in all cases. In the consistency analysis, PD-L1 expression in slices stored at room temperature for 2 weeks was consistent with PD-L1 expression in fresh slices (ICC ≥ 0.9 for all slices), and PD-L1 expression in slices stored at 4 °C or -20 °C for 4 weeks was consistent with PD-L1 expression in fresh slices (ICC ≥ 0.9 for all slices). When stored under refrigeration at -80 °C, PD-L1 expression in slices stored for 3 weeks was consistent with that in fresh slices (ICC ≥ 0.9). Conclusions To our knowledge, this is the first article on the effect of preservation time and preservation temperature of paraffin sections on PD-L1 expression in breast cancer. Long-term storage of paraffin sections of unstained invasive breast cancer can lead to antigen loss of PD-L1 (SP142). Refrigerated storage of paraffin sections can delay antigen loss, with best results at 4 °C or -20 °C, and a storage time of no more than 4 weeks is recommended.https://doi.org/10.1186/s13000-023-01423-8Breast cancerPD-L1ImmunohistochemistryParaffin sectionsStorage time |
| spellingShingle | Jiankun He Xinran Wang Lijing Cai Zhanli Jia Chang Liu Xuemei Sun Si Wu Chunyan Ding Zi Zhang Yueping Liu Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancer Diagnostic Pathology Breast cancer PD-L1 Immunohistochemistry Paraffin sections Storage time |
| title | Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancer |
| title_full | Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancer |
| title_fullStr | Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancer |
| title_full_unstemmed | Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancer |
| title_short | Effect of storage time of paraffin sections on the expression of PD-L1 (SP142) in invasive breast cancer |
| title_sort | effect of storage time of paraffin sections on the expression of pd l1 sp142 in invasive breast cancer |
| topic | Breast cancer PD-L1 Immunohistochemistry Paraffin sections Storage time |
| url | https://doi.org/10.1186/s13000-023-01423-8 |
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