Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil
Glässer’s disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific p...
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PeerJ Inc.
2019-05-01
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| Online Access: | https://peerj.com/articles/6817.pdf |
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| author | Julia Pires Espíndola Natalia Balbinott Letícia Trevisan Gressler Gustavo Machado Catia Silene Klein Raquel Rebelatto César Bernardo Gutiérrez Martín Luiz Carlos Kreutz Anthony Bernard Schryvers Rafael Frandoloso |
| author_facet | Julia Pires Espíndola Natalia Balbinott Letícia Trevisan Gressler Gustavo Machado Catia Silene Klein Raquel Rebelatto César Bernardo Gutiérrez Martín Luiz Carlos Kreutz Anthony Bernard Schryvers Rafael Frandoloso |
| author_sort | Julia Pires Espíndola |
| collection | DOAJ |
| description | Glässer’s disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer’s disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCR-based approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD. |
| first_indexed | 2024-03-09T06:26:33Z |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:26:33Z |
| publishDate | 2019-05-01 |
| publisher | PeerJ Inc. |
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| spelling | doaj.art-0af065031d5742e1a6363deeea0d242f2023-12-03T11:18:37ZengPeerJ Inc.PeerJ2167-83592019-05-017e681710.7717/peerj.6817Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in BrazilJulia Pires Espíndola0Natalia Balbinott1Letícia Trevisan Gressler2Gustavo Machado3Catia Silene Klein4Raquel Rebelatto5César Bernardo Gutiérrez Martín6Luiz Carlos Kreutz7Anthony Bernard Schryvers8Rafael Frandoloso9Laboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo, Rio Grande do Sul, BrazilLaboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo, Rio Grande do Sul, BrazilLaboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo, Rio Grande do Sul, BrazilDepartment of Population Health and Pathobiology, College of Veterinary Medicine, North Carolina State University, Raleigh, NC, United States of AmericaEmbrapa Suínos e Aves, Concórdia, Santa Catarina, BrazilEmbrapa Suínos e Aves, Concórdia, Santa Catarina, BrazilSection of Microbiology and Immunology, Department of Animal Health, Faculty of Veterinary, University of León, León, Castilla y León, SpainLaboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo, Rio Grande do Sul, BrazilDepartment of Microbiology & Infectious Diseases, Faculty of Medicine, University of Calgary, Calgary, Alberta, CanadaLaboratory of Microbiology and Advanced Immunology, Faculty of Agronomy and Veterinary Medicine, University of Passo Fundo, Passo Fundo, Rio Grande do Sul, BrazilGlässer’s disease (GD) is an important infectious disease of swine caused by Haemophilus (Glaesserella) parasuis. Vaccination with inactivated whole cell vaccines is the major approach for prevention of H. parasuis infection worldwide, but the immunity induced is predominantly against the specific polysaccharide capsule. As a consequence, the available vaccines may not induce adequate protection against the field strains, when the capsules present in the vaccine strains are different from those in strains isolated from the farms. Therefore, it is crucial to map H. parasuis serovars associated with regional outbreaks so that appropriate bacterin vaccines can be developed and distributed for prevention of infection. In this study, 459 H. parasuis field strains isolated from different Glässer’s disease outbreaks that occurred in 10 different Brazilian States were analyzed for serotype using PCR-based approaches. Surprisingly, non-typeable (NT) strains were the second most prevalent group of field strains and along with serovars 4, 5 and 1 comprised more than 70% of the isolates. A PCR-based approach designed to amplify the entire polysaccharide capsule locus revealed 9 different band patterns in the NT strains, and 75% of the NT strains belonged to three clusters, suggesting that a number of new serovars are responsible for a substantial proportion of disease. These results indicate that commercially available vaccines in Brazil do not cover the most prevalent H. parasuis serovars associated with GD.https://peerj.com/articles/6817.pdfDisease mappingHaemophilus parasuisTypificationSerovarsVaccinesBrazil |
| spellingShingle | Julia Pires Espíndola Natalia Balbinott Letícia Trevisan Gressler Gustavo Machado Catia Silene Klein Raquel Rebelatto César Bernardo Gutiérrez Martín Luiz Carlos Kreutz Anthony Bernard Schryvers Rafael Frandoloso Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil PeerJ Disease mapping Haemophilus parasuis Typification Serovars Vaccines Brazil |
| title | Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil |
| title_full | Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil |
| title_fullStr | Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil |
| title_full_unstemmed | Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil |
| title_short | Molecular serotyping of clinical strains of Haemophilus (Glaesserella) parasuis brings new insights regarding Glässer’s disease outbreaks in Brazil |
| title_sort | molecular serotyping of clinical strains of haemophilus glaesserella parasuis brings new insights regarding glasser s disease outbreaks in brazil |
| topic | Disease mapping Haemophilus parasuis Typification Serovars Vaccines Brazil |
| url | https://peerj.com/articles/6817.pdf |
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