Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin-derived aromatic compounds
Utilization of lignin, an abundant renewable resource, is limited by its heterogenous composition and complex structure. Biological valorization of lignin provides advantages over traditional chemical processing as it occurs at ambient temperature and pressure and does not use harsh chemicals. Furth...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-12-01
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| Series: | Metabolic Engineering Communications |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214030121000134 |
| _version_ | 1819177526783639552 |
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| author | Erika Arvay Bradley W. Biggs Laura Guerrero Virginia Jiang Keith Tyo |
| author_facet | Erika Arvay Bradley W. Biggs Laura Guerrero Virginia Jiang Keith Tyo |
| author_sort | Erika Arvay |
| collection | DOAJ |
| description | Utilization of lignin, an abundant renewable resource, is limited by its heterogenous composition and complex structure. Biological valorization of lignin provides advantages over traditional chemical processing as it occurs at ambient temperature and pressure and does not use harsh chemicals. Furthermore, the ability to biologically funnel heterogenous substrates to products eliminates the need for costly downstream processing and separation of feedstocks. However, lack of relevant metabolic networks and low tolerance to degradation products of lignin limits the application of traditional engineered model organisms. To circumvent this obstacle, we employed Acinetobacter baylyi ADP1, which natively catabolizes lignin-derived aromatic substrates through the β-ketoadipate pathway, to produce mevalonate from lignin-derived compounds. We enabled expression of the mevalonate pathway in ADP1 and validated activity in the presence of multiple lignin-derived aromatic substrates. Furthermore, by knocking out wax ester synthesis and utilizing fed-batch cultivation, we improved mevalonate titers 7.5-fold to 1014 mg/L (6.8 mM). This work establishes a foundation and provides groundwork for future efforts to engineer improved production of mevalonate and derivatives from lignin-derived aromatics using ADP1. |
| first_indexed | 2024-12-22T21:28:04Z |
| format | Article |
| id | doaj.art-0af54a48c83f4462856ccae015324cfd |
| institution | Directory Open Access Journal |
| issn | 2214-0301 |
| language | English |
| last_indexed | 2024-12-22T21:28:04Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Metabolic Engineering Communications |
| spelling | doaj.art-0af54a48c83f4462856ccae015324cfd2022-12-21T18:12:00ZengElsevierMetabolic Engineering Communications2214-03012021-12-0113e00173Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin-derived aromatic compoundsErika Arvay0Bradley W. Biggs1Laura Guerrero2Virginia Jiang3Keith Tyo4Department of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA; Biotechnology Training Program, Northwestern University, Evanston, IL, USADepartment of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA; Biotechnology Training Program, Northwestern University, Evanston, IL, USADepartment of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USADepartment of Chemical Engineering, Columbia University, New York, NY, USADepartment of Chemical and Biological Engineering, Northwestern University, Evanston, IL, USA; Corresponding author.Utilization of lignin, an abundant renewable resource, is limited by its heterogenous composition and complex structure. Biological valorization of lignin provides advantages over traditional chemical processing as it occurs at ambient temperature and pressure and does not use harsh chemicals. Furthermore, the ability to biologically funnel heterogenous substrates to products eliminates the need for costly downstream processing and separation of feedstocks. However, lack of relevant metabolic networks and low tolerance to degradation products of lignin limits the application of traditional engineered model organisms. To circumvent this obstacle, we employed Acinetobacter baylyi ADP1, which natively catabolizes lignin-derived aromatic substrates through the β-ketoadipate pathway, to produce mevalonate from lignin-derived compounds. We enabled expression of the mevalonate pathway in ADP1 and validated activity in the presence of multiple lignin-derived aromatic substrates. Furthermore, by knocking out wax ester synthesis and utilizing fed-batch cultivation, we improved mevalonate titers 7.5-fold to 1014 mg/L (6.8 mM). This work establishes a foundation and provides groundwork for future efforts to engineer improved production of mevalonate and derivatives from lignin-derived aromatics using ADP1.http://www.sciencedirect.com/science/article/pii/S2214030121000134Acinetobacter baylyi ADP1LigninMevalonateMetabolic engineeringRenewable chemistry |
| spellingShingle | Erika Arvay Bradley W. Biggs Laura Guerrero Virginia Jiang Keith Tyo Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin-derived aromatic compounds Metabolic Engineering Communications Acinetobacter baylyi ADP1 Lignin Mevalonate Metabolic engineering Renewable chemistry |
| title | Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin-derived aromatic compounds |
| title_full | Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin-derived aromatic compounds |
| title_fullStr | Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin-derived aromatic compounds |
| title_full_unstemmed | Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin-derived aromatic compounds |
| title_short | Engineering Acinetobacter baylyi ADP1 for mevalonate production from lignin-derived aromatic compounds |
| title_sort | engineering acinetobacter baylyi adp1 for mevalonate production from lignin derived aromatic compounds |
| topic | Acinetobacter baylyi ADP1 Lignin Mevalonate Metabolic engineering Renewable chemistry |
| url | http://www.sciencedirect.com/science/article/pii/S2214030121000134 |
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