| Summary: | In this study, maize plant was cultured in soil contaminated with different levels of crude oil. The purpose was to investigate the change of soil properties, leaf physiological and chemical parameters, and phenanthrene content in the leaf. Results showed that soil water content significantly increased when the levels of total petroleum hydrocarbons were 3700−17,800 mg/kg in soil, and soil electrical conductivity significantly increased compared with the control. In maize leaf, stomatal length and density, as well as K and Na contents decreased in contaminated treatments compared with the control. Stomatal length has a significant positive correlation with K content in leaf (r = 0.92, <i>p</i> < 0.01), while stomatal density was negatively correlated to the crude oil level in soil (r = −0.91, <i>p</i> < 0.05). Accumulation of phenanthrene in maize leaf was mainly through the foliar uptake pathway. Phenanthrene concentrations of maize leaf in oil-treated soil were less than that of the control, which exhibited a significant positive relationship with stomatal length (r = 0.98, <i>p</i> < 0.01). This study demonstrated that the stomata structure of maize could be influenced by crude oil and thus possibly controlling the accumulation of polycyclic aromatic hydrocarbons in aerial tissues. Based on these results, controlling stomata movement will be beneficial to phytoremediation of contaminated soil.
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