Molecular and Functional Characterization of Metabolites from Terrestrial Streptomyces hygroscopicus AVS7 Isolated from Western Ghats, India
Genus Streptomyces contributes to almost 80% of the world’s antibiotics under actinomycetes group. Pigments produced by this genus also been widely reported to have application in different field. This study was aimed in isolating a bio pigment having antimicrobial, anticancer and quorum quenchin...
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Journal of Pure and Applied Microbiology
2017-12-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/molecular-and-functional-characterization-of-metabolites-from-terrestrial/ |
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| author | V. Manon Mani A. Parimala Gnana Soundari S. Ashokraj V. Brindha Priyadarisini |
| author_facet | V. Manon Mani A. Parimala Gnana Soundari S. Ashokraj V. Brindha Priyadarisini |
| author_sort | V. Manon Mani |
| collection | DOAJ |
| description | Genus Streptomyces contributes to almost 80% of the world’s antibiotics under
actinomycetes group. Pigments produced by this genus also been widely reported to have
application in different field. This study was aimed in isolating a bio pigment having
antimicrobial, anticancer and quorum quenching properties. To achieve the aim soil samples
from the regions of Western Ghats around Coimbatore (TN, India) were collected which holds
biological diversity and rich in mineral wealth. The aim was attained through screening
of antagonisms for potential strain and the morphological, biochemical, physiological and
molecular characterization was investigated sequentially. Further, the biological studies were
evaluated. Totally 161 Actinomycetes strains were isolated from the collected samples out of
which potent 27 were pigmented Streptomyces sp. The potential strain was screened through
antagonism principle and the morphological, biochemical, physiological and molecular
characterization revealed that the strain is Streptomyces hygroscopicus AVS7 (KP732441). The
crude pigmented secondary metabolites were extracted using polar solvent methanol from the
biomass of the strain AVS7. The chemically characterized metabolite was a carboxylic acid
derivative compound. The crude methanolic metabolites extract which was reddish brown
in color showed maximum zone of inhibition against 6 different pathogenic organisms from
antimicrobial assessment. The crude extract showed quenching activity of the quorum sensing
molecules produced by Staphylococcus aureus. From this it is evident that the crude secondary
metabolites have the potentiality of being an antibiotic. The bioactive crude extract was found
to have biological properties at significant range and further bioprospecting activity will be
evaluated in future studies. |
| first_indexed | 2024-12-19T22:11:20Z |
| format | Article |
| id | doaj.art-0afc339c2e0b40fea3b063002cf1fd53 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-12-19T22:11:20Z |
| publishDate | 2017-12-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-0afc339c2e0b40fea3b063002cf1fd532022-12-21T20:03:53ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2017-12-011141823183110.22207/JPAM.11.4.21Molecular and Functional Characterization of Metabolites from Terrestrial Streptomyces hygroscopicus AVS7 Isolated from Western Ghats, IndiaV. Manon Mani0A. Parimala Gnana Soundari1S. Ashokraj2V. Brindha Priyadarisini31 Department of Biotechnology, Hindusthan College of Arts and Science, Coimbatore- 641028, TN, India. 2 Department of Microbial Biotechnology, Bharathiar University, Coimbatore - 641046, TN, India.2 Department of Microbial Biotechnology, Bharathiar University, Coimbatore - 641046, TN, India.3 Department of Horticulture, Sunchon National University, Jeonnam- 540950, Republic of Korea.2 Department of Microbial Biotechnology, Bharathiar University, Coimbatore - 641046, TN, India.Genus Streptomyces contributes to almost 80% of the world’s antibiotics under actinomycetes group. Pigments produced by this genus also been widely reported to have application in different field. This study was aimed in isolating a bio pigment having antimicrobial, anticancer and quorum quenching properties. To achieve the aim soil samples from the regions of Western Ghats around Coimbatore (TN, India) were collected which holds biological diversity and rich in mineral wealth. The aim was attained through screening of antagonisms for potential strain and the morphological, biochemical, physiological and molecular characterization was investigated sequentially. Further, the biological studies were evaluated. Totally 161 Actinomycetes strains were isolated from the collected samples out of which potent 27 were pigmented Streptomyces sp. The potential strain was screened through antagonism principle and the morphological, biochemical, physiological and molecular characterization revealed that the strain is Streptomyces hygroscopicus AVS7 (KP732441). The crude pigmented secondary metabolites were extracted using polar solvent methanol from the biomass of the strain AVS7. The chemically characterized metabolite was a carboxylic acid derivative compound. The crude methanolic metabolites extract which was reddish brown in color showed maximum zone of inhibition against 6 different pathogenic organisms from antimicrobial assessment. The crude extract showed quenching activity of the quorum sensing molecules produced by Staphylococcus aureus. From this it is evident that the crude secondary metabolites have the potentiality of being an antibiotic. The bioactive crude extract was found to have biological properties at significant range and further bioprospecting activity will be evaluated in future studies.https://microbiologyjournal.org/molecular-and-functional-characterization-of-metabolites-from-terrestrial/antagonistmicpigmentsquorum quenchingstreptomyces sp. |
| spellingShingle | V. Manon Mani A. Parimala Gnana Soundari S. Ashokraj V. Brindha Priyadarisini Molecular and Functional Characterization of Metabolites from Terrestrial Streptomyces hygroscopicus AVS7 Isolated from Western Ghats, India Journal of Pure and Applied Microbiology antagonist mic pigments quorum quenching streptomyces sp. |
| title | Molecular and Functional Characterization of Metabolites from Terrestrial Streptomyces hygroscopicus AVS7 Isolated from Western Ghats, India |
| title_full | Molecular and Functional Characterization of Metabolites from Terrestrial Streptomyces hygroscopicus AVS7 Isolated from Western Ghats, India |
| title_fullStr | Molecular and Functional Characterization of Metabolites from Terrestrial Streptomyces hygroscopicus AVS7 Isolated from Western Ghats, India |
| title_full_unstemmed | Molecular and Functional Characterization of Metabolites from Terrestrial Streptomyces hygroscopicus AVS7 Isolated from Western Ghats, India |
| title_short | Molecular and Functional Characterization of Metabolites from Terrestrial Streptomyces hygroscopicus AVS7 Isolated from Western Ghats, India |
| title_sort | molecular and functional characterization of metabolites from terrestrial streptomyces hygroscopicus avs7 isolated from western ghats india |
| topic | antagonist mic pigments quorum quenching streptomyces sp. |
| url | https://microbiologyjournal.org/molecular-and-functional-characterization-of-metabolites-from-terrestrial/ |
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