Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhi
Lactobacillus plantarum U10 produced bacteriocin U10 which was isolated from a traditionally fermented food “tempoyak” from Sumatera Island in Indonesia. Production of the bacteriocins started at early exponential phase and reached maximum level at early stationary phase. Furthermore, plantaricins U...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indonesian Society for Microbiology
2015-06-01
|
| Series: | Microbiology Indonesia |
| Subjects: | |
| Online Access: | http://jurnal.permi.or.id/index.php/mionline/article/view/323 |
| _version_ | 1828140295904034816 |
|---|---|
| author | SOGANDI APON ZAENAL MUSTOPA I MADE ARTIKA BUGI RATNO BUDIARTO |
| author_facet | SOGANDI APON ZAENAL MUSTOPA I MADE ARTIKA BUGI RATNO BUDIARTO |
| author_sort | SOGANDI |
| collection | DOAJ |
| description | Lactobacillus plantarum U10 produced bacteriocin U10 which was isolated from a traditionally fermented food “tempoyak” from Sumatera Island in Indonesia. Production of the bacteriocins started at early exponential phase and reached maximum level at early stationary phase. Furthermore, plantaricins U10 was purified by ammonium sulphate precipitation followed by gel filtration chromatography. L. plantarum U10 produced two bacteriocins with a molecular mass of approximately 4.5 and 9.8 kDa by SDS-PAGE. The mode of action of plantaricins U10 was identified as bactericidal agents against Salmonella typhi ATCC25241 as proven by CFU counting and SEM micrographs that showed differences in cell structures between treated cells and the non-treated control. SEM examination also confirmed structural destruction of membrane cells integrity and considerable morphological alteration of S.typhi. |
| first_indexed | 2024-04-11T19:06:58Z |
| format | Article |
| id | doaj.art-0afd730c7db24f56a7ad1b762e9264ac |
| institution | Directory Open Access Journal |
| issn | 1978-3477 2087-8575 |
| language | English |
| last_indexed | 2024-04-11T19:06:58Z |
| publishDate | 2015-06-01 |
| publisher | Indonesian Society for Microbiology |
| record_format | Article |
| series | Microbiology Indonesia |
| spelling | doaj.art-0afd730c7db24f56a7ad1b762e9264ac2022-12-22T04:07:43ZengIndonesian Society for MicrobiologyMicrobiology Indonesia1978-34772087-85752015-06-0192738110.5454/mi.9.2.4Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhiSOGANDI0APON ZAENAL MUSTOPA1I MADE ARTIKA2BUGI RATNO BUDIARTO3Department of Biochemistry, Institut Pertanian Bogor, Dramaga Campus, Bogor 16680, IndonesiaResearch Centre for Biotechnology, Indonesian Institute of Science (LIPI), Jalan Raya Bogor Km 46, Cibinong, Bogor 16911, IndonesiaDepartment of Biochemistry, Institut Pertanian Bogor, Dramaga Campus, Bogor 16680, IndonesiaResearch Centre for Biotechnology, Indonesian Institute of Science (LIPI), Jalan Raya Bogor Km 46, Cibinong, Bogor 16911, IndonesiaLactobacillus plantarum U10 produced bacteriocin U10 which was isolated from a traditionally fermented food “tempoyak” from Sumatera Island in Indonesia. Production of the bacteriocins started at early exponential phase and reached maximum level at early stationary phase. Furthermore, plantaricins U10 was purified by ammonium sulphate precipitation followed by gel filtration chromatography. L. plantarum U10 produced two bacteriocins with a molecular mass of approximately 4.5 and 9.8 kDa by SDS-PAGE. The mode of action of plantaricins U10 was identified as bactericidal agents against Salmonella typhi ATCC25241 as proven by CFU counting and SEM micrographs that showed differences in cell structures between treated cells and the non-treated control. SEM examination also confirmed structural destruction of membrane cells integrity and considerable morphological alteration of S.typhi.http://jurnal.permi.or.id/index.php/mionline/article/view/323bacteriocinL. plantarum U10mode of actionpurification |
| spellingShingle | SOGANDI APON ZAENAL MUSTOPA I MADE ARTIKA BUGI RATNO BUDIARTO Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhi Microbiology Indonesia bacteriocin L. plantarum U10 mode of action purification |
| title | Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhi |
| title_full | Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhi |
| title_fullStr | Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhi |
| title_full_unstemmed | Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhi |
| title_short | Inhibitory activity of Lactobacillus plantarum U10 isolated from Tempoyak (fermented durian) Made in Indonesia against Salmonella typhi |
| title_sort | inhibitory activity of lactobacillus plantarum u10 isolated from tempoyak fermented durian made in indonesia against salmonella typhi |
| topic | bacteriocin L. plantarum U10 mode of action purification |
| url | http://jurnal.permi.or.id/index.php/mionline/article/view/323 |
| work_keys_str_mv | AT sogandi inhibitoryactivityoflactobacillusplantarumu10isolatedfromtempoyakfermenteddurianmadeinindonesiaagainstsalmonellatyphi AT aponzaenalmustopa inhibitoryactivityoflactobacillusplantarumu10isolatedfromtempoyakfermenteddurianmadeinindonesiaagainstsalmonellatyphi AT imadeartika inhibitoryactivityoflactobacillusplantarumu10isolatedfromtempoyakfermenteddurianmadeinindonesiaagainstsalmonellatyphi AT bugiratnobudiarto inhibitoryactivityoflactobacillusplantarumu10isolatedfromtempoyakfermenteddurianmadeinindonesiaagainstsalmonellatyphi |