CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice
Common methods for the generation of human embryonic-derived neural stem cells (hNSCs) result in cells with potentially compromised safety profiles due to maintenance of cells in conditions containing non-human proteins (e.g. in bovine serum or on mouse fibroblast feeders). Additionally, sufficient...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2014-09-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506114000816 |
| _version_ | 1819042792998961152 |
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| author | Daniel L. Haus Hal X. Nguyen Eric M. Gold Noriko Kamei Harvey Perez Harry D. Moore Aileen J. Anderson Brian J. Cummings |
| author_facet | Daniel L. Haus Hal X. Nguyen Eric M. Gold Noriko Kamei Harvey Perez Harry D. Moore Aileen J. Anderson Brian J. Cummings |
| author_sort | Daniel L. Haus |
| collection | DOAJ |
| description | Common methods for the generation of human embryonic-derived neural stem cells (hNSCs) result in cells with potentially compromised safety profiles due to maintenance of cells in conditions containing non-human proteins (e.g. in bovine serum or on mouse fibroblast feeders). Additionally, sufficient expansion of resulting hNSCs for scaling out or up in a clinically relevant time frame has proven to be difficult. Here, we report a strategy that produces hNSCs in completely “Xeno-Free” culture conditions. Furthermore, we have enriched the hNSCs for the cell surface marker CD133 via magnetic sorting, which has led to an increase in the expansion rate and neuronal fate specification of the hNSCs in vitro. Critically, we have also confirmed neural lineage specificity upon sorted hNSC transplantation into the immunodeficient NOD-scid mouse brain. The future use or adaptation of these protocols has the potential to better facilitate the advancement of pre-clinical strategies from the bench to the bedside. |
| first_indexed | 2024-12-21T09:46:32Z |
| format | Article |
| id | doaj.art-0afe92b0566e46269311a9bc172a542f |
| institution | Directory Open Access Journal |
| issn | 1873-5061 1876-7753 |
| language | English |
| last_indexed | 2024-12-21T09:46:32Z |
| publishDate | 2014-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-0afe92b0566e46269311a9bc172a542f2022-12-21T19:08:19ZengElsevierStem Cell Research1873-50611876-77532014-09-0113221422610.1016/j.scr.2014.06.008CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient miceDaniel L. Haus0Hal X. Nguyen1Eric M. Gold2Noriko Kamei3Harvey Perez4Harry D. Moore5Aileen J. Anderson6Brian J. Cummings7Sue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USASue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USASue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USASue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USASue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USACentre for Stem Cell Biology, University of Sheffield, Sheffield S10 2TN, UKSue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USASue & Bill Gross Stem Cell Center, University of California, Irvine, CA 92697-1750, USACommon methods for the generation of human embryonic-derived neural stem cells (hNSCs) result in cells with potentially compromised safety profiles due to maintenance of cells in conditions containing non-human proteins (e.g. in bovine serum or on mouse fibroblast feeders). Additionally, sufficient expansion of resulting hNSCs for scaling out or up in a clinically relevant time frame has proven to be difficult. Here, we report a strategy that produces hNSCs in completely “Xeno-Free” culture conditions. Furthermore, we have enriched the hNSCs for the cell surface marker CD133 via magnetic sorting, which has led to an increase in the expansion rate and neuronal fate specification of the hNSCs in vitro. Critically, we have also confirmed neural lineage specificity upon sorted hNSC transplantation into the immunodeficient NOD-scid mouse brain. The future use or adaptation of these protocols has the potential to better facilitate the advancement of pre-clinical strategies from the bench to the bedside.http://www.sciencedirect.com/science/article/pii/S1873506114000816 |
| spellingShingle | Daniel L. Haus Hal X. Nguyen Eric M. Gold Noriko Kamei Harvey Perez Harry D. Moore Aileen J. Anderson Brian J. Cummings CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice Stem Cell Research |
| title | CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice |
| title_full | CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice |
| title_fullStr | CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice |
| title_full_unstemmed | CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice |
| title_short | CD133-enriched Xeno-Free human embryonic-derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice |
| title_sort | cd133 enriched xeno free human embryonic derived neural stem cells expand rapidly in culture and do not form teratomas in immunodeficient mice |
| url | http://www.sciencedirect.com/science/article/pii/S1873506114000816 |
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