A Systematic Evaluation of the SARS-CoV-2 Vaccine-Induced Anti-S-RBD-Ig Response in a Population of Health Care Workers
In the wake of the COVID-19 pandemic, the novel class of mRNA vaccines has been granted first-time approval for active immunization against SARS-CoV-2 alongside the already established viral vector-based vaccines. In this prospective single-center study, we set out to determine the vaccine-induced h...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-09-01
|
| Series: | Vaccines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-393X/11/9/1467 |
| _version_ | 1797576453335810048 |
|---|---|
| author | Viktoria Hentschel Cornelia Horsch Benjamin Mayer Annsophie Thies Will Qian Joris Kroschel Thomas Seufferlein Lukas Perkhofer Martin Müller |
| author_facet | Viktoria Hentschel Cornelia Horsch Benjamin Mayer Annsophie Thies Will Qian Joris Kroschel Thomas Seufferlein Lukas Perkhofer Martin Müller |
| author_sort | Viktoria Hentschel |
| collection | DOAJ |
| description | In the wake of the COVID-19 pandemic, the novel class of mRNA vaccines has been granted first-time approval for active immunization against SARS-CoV-2 alongside the already established viral vector-based vaccines. In this prospective single-center study, we set out to determine the vaccine-induced humoral immune response in a population of 1512 health care employees after the second and third vaccination, respectively. Anti-SARS-CoV-2 receptor-binding domain (RBD) and nucleocapsid antigen antibody concentrations were assessed using commercially available immunoassays. We could show that, in particular, young study subjects aged below 30 years, as well as those with a prior SARS-CoV-2 infection, developed significantly higher antibody concentrations. Our data further suggest that being in physically close contact with formerly SARS-CoV-2-positive people positively affects the post-vaccination response. Surprisingly, study subjects with a BMI > 30 produced the highest anti-S-RBD Ig antibody levels if they had recently received their third vaccination. Also, heterologous dual vaccine regimens consisting of a BNT162b2 and ChAdOx1 n-CoV-19, a homologous triple combination of BNT162b2, and an application of mRNA-1273 as the third vaccine, were most efficient at eliciting a humoral immune response. Our study substantiates existing evidence, but beyond that, scrutinizes the impact of vaccine agents and their respective combinations, as well as different time intervals on humoral immunogenicity. |
| first_indexed | 2024-03-10T21:52:08Z |
| format | Article |
| id | doaj.art-0affd3fc9af342d9b76f2b2f102c1d4d |
| institution | Directory Open Access Journal |
| issn | 2076-393X |
| language | English |
| last_indexed | 2024-03-10T21:52:08Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Vaccines |
| spelling | doaj.art-0affd3fc9af342d9b76f2b2f102c1d4d2023-11-19T13:19:22ZengMDPI AGVaccines2076-393X2023-09-01119146710.3390/vaccines11091467A Systematic Evaluation of the SARS-CoV-2 Vaccine-Induced Anti-S-RBD-Ig Response in a Population of Health Care WorkersViktoria Hentschel0Cornelia Horsch1Benjamin Mayer2Annsophie Thies3Will Qian4Joris Kroschel5Thomas Seufferlein6Lukas Perkhofer7Martin Müller8Department of Internal Medicine I, Ulm University Hospital, 89081 Ulm, GermanyInstitute for Epidemiology and Medical Biometry, Ulm University, 89075 Ulm, GermanyInstitute for Epidemiology and Medical Biometry, Ulm University, 89075 Ulm, GermanyDepartment of Internal Medicine I, Ulm University Hospital, 89081 Ulm, GermanyDepartment of Internal Medicine I, Ulm University Hospital, 89081 Ulm, GermanyCentral Department of Clinical Chemistry, Ulm University Hospital, 89081 Ulm, GermanyDepartment of Internal Medicine I, Ulm University Hospital, 89081 Ulm, GermanyDepartment of Internal Medicine I, Ulm University Hospital, 89081 Ulm, GermanyDepartment of Internal Medicine I, Ulm University Hospital, 89081 Ulm, GermanyIn the wake of the COVID-19 pandemic, the novel class of mRNA vaccines has been granted first-time approval for active immunization against SARS-CoV-2 alongside the already established viral vector-based vaccines. In this prospective single-center study, we set out to determine the vaccine-induced humoral immune response in a population of 1512 health care employees after the second and third vaccination, respectively. Anti-SARS-CoV-2 receptor-binding domain (RBD) and nucleocapsid antigen antibody concentrations were assessed using commercially available immunoassays. We could show that, in particular, young study subjects aged below 30 years, as well as those with a prior SARS-CoV-2 infection, developed significantly higher antibody concentrations. Our data further suggest that being in physically close contact with formerly SARS-CoV-2-positive people positively affects the post-vaccination response. Surprisingly, study subjects with a BMI > 30 produced the highest anti-S-RBD Ig antibody levels if they had recently received their third vaccination. Also, heterologous dual vaccine regimens consisting of a BNT162b2 and ChAdOx1 n-CoV-19, a homologous triple combination of BNT162b2, and an application of mRNA-1273 as the third vaccine, were most efficient at eliciting a humoral immune response. Our study substantiates existing evidence, but beyond that, scrutinizes the impact of vaccine agents and their respective combinations, as well as different time intervals on humoral immunogenicity.https://www.mdpi.com/2076-393X/11/9/1467SARS-CoV-2humoral immune responsemRNA vaccines |
| spellingShingle | Viktoria Hentschel Cornelia Horsch Benjamin Mayer Annsophie Thies Will Qian Joris Kroschel Thomas Seufferlein Lukas Perkhofer Martin Müller A Systematic Evaluation of the SARS-CoV-2 Vaccine-Induced Anti-S-RBD-Ig Response in a Population of Health Care Workers Vaccines SARS-CoV-2 humoral immune response mRNA vaccines |
| title | A Systematic Evaluation of the SARS-CoV-2 Vaccine-Induced Anti-S-RBD-Ig Response in a Population of Health Care Workers |
| title_full | A Systematic Evaluation of the SARS-CoV-2 Vaccine-Induced Anti-S-RBD-Ig Response in a Population of Health Care Workers |
| title_fullStr | A Systematic Evaluation of the SARS-CoV-2 Vaccine-Induced Anti-S-RBD-Ig Response in a Population of Health Care Workers |
| title_full_unstemmed | A Systematic Evaluation of the SARS-CoV-2 Vaccine-Induced Anti-S-RBD-Ig Response in a Population of Health Care Workers |
| title_short | A Systematic Evaluation of the SARS-CoV-2 Vaccine-Induced Anti-S-RBD-Ig Response in a Population of Health Care Workers |
| title_sort | systematic evaluation of the sars cov 2 vaccine induced anti s rbd ig response in a population of health care workers |
| topic | SARS-CoV-2 humoral immune response mRNA vaccines |
| url | https://www.mdpi.com/2076-393X/11/9/1467 |
| work_keys_str_mv | AT viktoriahentschel asystematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT corneliahorsch asystematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT benjaminmayer asystematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT annsophiethies asystematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT willqian asystematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT joriskroschel asystematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT thomasseufferlein asystematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT lukasperkhofer asystematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT martinmuller asystematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT viktoriahentschel systematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT corneliahorsch systematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT benjaminmayer systematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT annsophiethies systematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT willqian systematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT joriskroschel systematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT thomasseufferlein systematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT lukasperkhofer systematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers AT martinmuller systematicevaluationofthesarscov2vaccineinducedantisrbdigresponseinapopulationofhealthcareworkers |