A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast

A new protocol for single-cell RNA-seq reveals stochastic gene expression during lag phase in budding yeast

Current methods for single-cell RNA sequencing (scRNA-seq) of yeast cells do not match the throughput and relative simplicity of the state-of-the-art techniques that are available for mammalian cells. In this study, we report how 10x Genomics’ droplet-based single-cell RNA sequencing technology can...

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Bibliographic Details
Main Authors: Abbas Jariani, Lieselotte Vermeersch, Bram Cerulus, Gemma Perez-Samper, Karin Voordeckers, Thomas Van Brussel, Bernard Thienpont, Diether Lambrechts, Kevin J Verstrepen
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-05-01
Series:eLife
Subjects:
single-cell RNA-seq
lag phase
heterogeneity
tools & methods
metabolic shift
Online Access:https://elifesciences.org/articles/55320

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