Effects of soil salinity on rhizosphere soil microbes in transgenic Bt cotton fields
With increased cultivation of transgenic Bacillus thuringiensis (Bt) cotton in the saline alkaline soil of China, assessments of transgenic crop biosafety have focused on the effects of soil salinity on rhizosphere microbes and Bt protein residues. In 2013 and 2014, investigations were conducted on...
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| Format: | Article |
| Language: | English |
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Elsevier
2017-07-01
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| Series: | Journal of Integrative Agriculture |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311916614569 |
| _version_ | 1819063942496911360 |
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| author | Jun-yu LUO Shuai ZHANG Xiang-zhen ZHU Li-min LU Chun-yi WANG Chun-hua LI Jin-jie CUI Zhi-guo ZHOU |
| author_facet | Jun-yu LUO Shuai ZHANG Xiang-zhen ZHU Li-min LU Chun-yi WANG Chun-hua LI Jin-jie CUI Zhi-guo ZHOU |
| author_sort | Jun-yu LUO |
| collection | DOAJ |
| description | With increased cultivation of transgenic Bacillus thuringiensis (Bt) cotton in the saline alkaline soil of China, assessments of transgenic crop biosafety have focused on the effects of soil salinity on rhizosphere microbes and Bt protein residues. In 2013 and 2014, investigations were conducted on the rhizosphere microbial biomass, soil enzyme activities and Bt protein contents of the soil under transgenic Bt cotton (variety GK19) and its parental non-transgenic cotton (Simian 3) cultivated at various salinity levels (1.15, 6.00 and 11.46 dS m−1). Under soil salinity stress, trace amounts of Bt proteins were observed in the Bt cotton GK19 rhizosphere soil, although the protein content increased with cotton growth and increased soil salinity levels. The populations of slight halophilic bacteria, phosphate solubilizing bacteria, ammonifying bacteria, nitrifying bacteria and denitrifying bacteria decreased with increased soil salinity in the Bt and non-Bt cotton rhizosphere soil, and the microbial biomass carbon, microbial respiration and soil catalase, urease and alkaline phosphatase activity also decreased. Correlation analyses showed that the increased Bt protein content in the Bt cotton rhizosphere soil may have been caused by the slower decomposition of soil microorganisms, which suggests that salinity was the main factor influencing the relevant activities of the soil microorganisms and indicates that Bt proteins had no clear adverse effects on the soil microorganisms. The results of this study may provide a theoretical basis for risk assessments of genetically modified cotton in saline alkaline soil. |
| first_indexed | 2024-12-21T15:22:41Z |
| format | Article |
| id | doaj.art-0b07c0398a774d8a84c230cb988d6f51 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-21T15:22:41Z |
| publishDate | 2017-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-0b07c0398a774d8a84c230cb988d6f512022-12-21T18:59:00ZengElsevierJournal of Integrative Agriculture2095-31192017-07-0116716241633Effects of soil salinity on rhizosphere soil microbes in transgenic Bt cotton fieldsJun-yu LUO0Shuai ZHANG1Xiang-zhen ZHU2Li-min LU3Chun-yi WANG4Chun-hua LI5Jin-jie CUI6Zhi-guo ZHOU7State Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China; College of Agriculture, Nanjing Agricultural University/Key Laboratory of Crop Physiology & Ecology in Southern China, Ministry of Agriculture, Nanjing 210095, P.R.ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.ChinaState Key Laboratory of Cotton Biology, Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, P.R.China; Correspondence CUI Jin-jieCollege of Agriculture, Nanjing Agricultural University/Key Laboratory of Crop Physiology & Ecology in Southern China, Ministry of Agriculture, Nanjing 210095, P.R.ChinaWith increased cultivation of transgenic Bacillus thuringiensis (Bt) cotton in the saline alkaline soil of China, assessments of transgenic crop biosafety have focused on the effects of soil salinity on rhizosphere microbes and Bt protein residues. In 2013 and 2014, investigations were conducted on the rhizosphere microbial biomass, soil enzyme activities and Bt protein contents of the soil under transgenic Bt cotton (variety GK19) and its parental non-transgenic cotton (Simian 3) cultivated at various salinity levels (1.15, 6.00 and 11.46 dS m−1). Under soil salinity stress, trace amounts of Bt proteins were observed in the Bt cotton GK19 rhizosphere soil, although the protein content increased with cotton growth and increased soil salinity levels. The populations of slight halophilic bacteria, phosphate solubilizing bacteria, ammonifying bacteria, nitrifying bacteria and denitrifying bacteria decreased with increased soil salinity in the Bt and non-Bt cotton rhizosphere soil, and the microbial biomass carbon, microbial respiration and soil catalase, urease and alkaline phosphatase activity also decreased. Correlation analyses showed that the increased Bt protein content in the Bt cotton rhizosphere soil may have been caused by the slower decomposition of soil microorganisms, which suggests that salinity was the main factor influencing the relevant activities of the soil microorganisms and indicates that Bt proteins had no clear adverse effects on the soil microorganisms. The results of this study may provide a theoretical basis for risk assessments of genetically modified cotton in saline alkaline soil.http://www.sciencedirect.com/science/article/pii/S2095311916614569soil salinityBt cottonsoil microorganismsmicrobial biomass carbonmicrobial respirationsoil enzyme activity |
| spellingShingle | Jun-yu LUO Shuai ZHANG Xiang-zhen ZHU Li-min LU Chun-yi WANG Chun-hua LI Jin-jie CUI Zhi-guo ZHOU Effects of soil salinity on rhizosphere soil microbes in transgenic Bt cotton fields Journal of Integrative Agriculture soil salinity Bt cotton soil microorganisms microbial biomass carbon microbial respiration soil enzyme activity |
| title | Effects of soil salinity on rhizosphere soil microbes in transgenic Bt cotton fields |
| title_full | Effects of soil salinity on rhizosphere soil microbes in transgenic Bt cotton fields |
| title_fullStr | Effects of soil salinity on rhizosphere soil microbes in transgenic Bt cotton fields |
| title_full_unstemmed | Effects of soil salinity on rhizosphere soil microbes in transgenic Bt cotton fields |
| title_short | Effects of soil salinity on rhizosphere soil microbes in transgenic Bt cotton fields |
| title_sort | effects of soil salinity on rhizosphere soil microbes in transgenic bt cotton fields |
| topic | soil salinity Bt cotton soil microorganisms microbial biomass carbon microbial respiration soil enzyme activity |
| url | http://www.sciencedirect.com/science/article/pii/S2095311916614569 |
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