Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostella
The interactions between Arabidopsis thaliana and Plutella xylostella have been considered as a model system to unravel the responses of plants to herbivorous insects. Here, we use a 2-DE proteome approach to detect protein expression changes in the leaves of Arabidopsis plants exposed to P. xyloste...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2018-01-01
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| Series: | Journal of Plant Interactions |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/17429145.2017.1414320 |
| _version_ | 1818477519573090304 |
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| author | Dieu-Hien Truong Hoang Chinh Nguyen Julien Bauwens Gabriel Mazzucchelli Georges Lognay Frédéric Francis |
| author_facet | Dieu-Hien Truong Hoang Chinh Nguyen Julien Bauwens Gabriel Mazzucchelli Georges Lognay Frédéric Francis |
| author_sort | Dieu-Hien Truong |
| collection | DOAJ |
| description | The interactions between Arabidopsis thaliana and Plutella xylostella have been considered as a model system to unravel the responses of plants to herbivorous insects. Here, we use a 2-DE proteome approach to detect protein expression changes in the leaves of Arabidopsis plants exposed to P. xylostella larval infestation at 27°C within 8 h. Approximately 450 protein spots were reproducibly detected on gels. Of these, comparing healthy and infested leaves, we identified 18 differentially expressed protein spots. Thirteen proteins were successfully identified by MALDI-TOF/MS and LC-ESI-MS/MS. Functional classification analysis indicated that the differentially identified proteins were associated with amino acid, carbohydrate, energy, lipid metabolism, and photosynthesis. In addition, their relative abundances were assessed according to larval pest feeding on Arabidopsis leaves. These data provide valuable new insights for further works in plant-biotic and environmental stress interaction. |
| first_indexed | 2024-12-10T09:38:43Z |
| format | Article |
| id | doaj.art-0b0854031f5845959fbddee0dec8c243 |
| institution | Directory Open Access Journal |
| issn | 1742-9145 1742-9153 |
| language | English |
| last_indexed | 2024-12-10T09:38:43Z |
| publishDate | 2018-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Journal of Plant Interactions |
| spelling | doaj.art-0b0854031f5845959fbddee0dec8c2432022-12-22T01:54:05ZengTaylor & Francis GroupJournal of Plant Interactions1742-91451742-91532018-01-01131303610.1080/17429145.2017.14143201414320Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostellaDieu-Hien Truong0Hoang Chinh Nguyen1Julien Bauwens2Gabriel Mazzucchelli3Georges Lognay4Frédéric Francis5Ton Duc Thang UniversityTon Duc Thang UniversityUniversity of Liège, Gembloux Agro-Bio TechUniversity of LiègeUniversity of Liège, Gembloux Agro-Bio TechUniversity of Liège, Gembloux Agro-Bio TechThe interactions between Arabidopsis thaliana and Plutella xylostella have been considered as a model system to unravel the responses of plants to herbivorous insects. Here, we use a 2-DE proteome approach to detect protein expression changes in the leaves of Arabidopsis plants exposed to P. xylostella larval infestation at 27°C within 8 h. Approximately 450 protein spots were reproducibly detected on gels. Of these, comparing healthy and infested leaves, we identified 18 differentially expressed protein spots. Thirteen proteins were successfully identified by MALDI-TOF/MS and LC-ESI-MS/MS. Functional classification analysis indicated that the differentially identified proteins were associated with amino acid, carbohydrate, energy, lipid metabolism, and photosynthesis. In addition, their relative abundances were assessed according to larval pest feeding on Arabidopsis leaves. These data provide valuable new insights for further works in plant-biotic and environmental stress interaction.http://dx.doi.org/10.1080/17429145.2017.1414320Arabidopsis thalianaPlutella xylostellaproteomic expression2-DEMALDI-TOF MSLC-ESI-MS/MS |
| spellingShingle | Dieu-Hien Truong Hoang Chinh Nguyen Julien Bauwens Gabriel Mazzucchelli Georges Lognay Frédéric Francis Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostella Journal of Plant Interactions Arabidopsis thaliana Plutella xylostella proteomic expression 2-DE MALDI-TOF MS LC-ESI-MS/MS |
| title | Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostella |
| title_full | Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostella |
| title_fullStr | Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostella |
| title_full_unstemmed | Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostella |
| title_short | Plant defense in response to chewing insects: proteome analysis of Arabidopsis thaliana damaged by Plutella xylostella |
| title_sort | plant defense in response to chewing insects proteome analysis of arabidopsis thaliana damaged by plutella xylostella |
| topic | Arabidopsis thaliana Plutella xylostella proteomic expression 2-DE MALDI-TOF MS LC-ESI-MS/MS |
| url | http://dx.doi.org/10.1080/17429145.2017.1414320 |
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