Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker)
Olfaction in insects has a critical role in recognizing the host, finding food, and choosing mating partners, as well as avoiding predators. Odorant receptors (ORs), which are housed in the dendritic membrane of sensory neurons and extended into the lymph of sensilla on insect antennae, are particip...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-04-01
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| Series: | Frontiers in Physiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fphys.2019.00367/full |
| _version_ | 1818548347360772096 |
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| author | Kunpeng Zhang Kunpeng Zhang Yilu Feng Lixiao Du Shanshan Gao Hang Yan Kun Li Nana Liu Junxiang Wu Guirong Wang |
| author_facet | Kunpeng Zhang Kunpeng Zhang Yilu Feng Lixiao Du Shanshan Gao Hang Yan Kun Li Nana Liu Junxiang Wu Guirong Wang |
| author_sort | Kunpeng Zhang |
| collection | DOAJ |
| description | Olfaction in insects has a critical role in recognizing the host, finding food, and choosing mating partners, as well as avoiding predators. Odorant receptors (ORs), which are housed in the dendritic membrane of sensory neurons and extended into the lymph of sensilla on insect antennae, are participating in the detection of volatile compounds in insects. In the present study, we identified an OR gene, named MsepOR13, in the oriental armyworm Mythimna separata (Walker). Quantitative real-time polymerase chain reaction revealed that MsepOR13 was expressed mainly in the antennae of male and female moths. In in vitro heterologous expression experiments, MsepOR13 was widely tuned to 32 of the 67 different compounds tested. Furthermore, MsepOR13 responded to eugenol at a low concentration of 10-9 M, with an EC50 value of 3.91 × 10-6 M. The high sensitivity suggests an important role for the OR13 gene in the moth olfactory system. |
| first_indexed | 2024-12-12T08:18:43Z |
| format | Article |
| id | doaj.art-0b08a78e336b4416bec0c9ed41802c31 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-12T08:18:43Z |
| publishDate | 2019-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-0b08a78e336b4416bec0c9ed41802c312022-12-22T00:31:27ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2019-04-011010.3389/fphys.2019.00367442463Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker)Kunpeng Zhang0Kunpeng Zhang1Yilu Feng2Lixiao Du3Shanshan Gao4Hang Yan5Kun Li6Nana Liu7Junxiang Wu8Guirong Wang9State Key Laboratory of Crop Stress, Northwest A&F University, Yangling, ChinaCollege of Biology and Food Engineering, Anyang Institute of Technology, Anyang, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaCollege of Biology and Food Engineering, Anyang Institute of Technology, Anyang, ChinaCollege of Biology and Food Engineering, Anyang Institute of Technology, Anyang, ChinaCollege of Biology and Food Engineering, Anyang Institute of Technology, Anyang, ChinaCollege of Biology and Food Engineering, Anyang Institute of Technology, Anyang, ChinaState Key Laboratory of Crop Stress, Northwest A&F University, Yangling, ChinaState Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, ChinaOlfaction in insects has a critical role in recognizing the host, finding food, and choosing mating partners, as well as avoiding predators. Odorant receptors (ORs), which are housed in the dendritic membrane of sensory neurons and extended into the lymph of sensilla on insect antennae, are participating in the detection of volatile compounds in insects. In the present study, we identified an OR gene, named MsepOR13, in the oriental armyworm Mythimna separata (Walker). Quantitative real-time polymerase chain reaction revealed that MsepOR13 was expressed mainly in the antennae of male and female moths. In in vitro heterologous expression experiments, MsepOR13 was widely tuned to 32 of the 67 different compounds tested. Furthermore, MsepOR13 responded to eugenol at a low concentration of 10-9 M, with an EC50 value of 3.91 × 10-6 M. The high sensitivity suggests an important role for the OR13 gene in the moth olfactory system.https://www.frontiersin.org/article/10.3389/fphys.2019.00367/fullMythimna separataodorant receptoreugenolXenopus oocytesodorant tuning |
| spellingShingle | Kunpeng Zhang Kunpeng Zhang Yilu Feng Lixiao Du Shanshan Gao Hang Yan Kun Li Nana Liu Junxiang Wu Guirong Wang Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker) Frontiers in Physiology Mythimna separata odorant receptor eugenol Xenopus oocytes odorant tuning |
| title | Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker) |
| title_full | Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker) |
| title_fullStr | Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker) |
| title_full_unstemmed | Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker) |
| title_short | Functional Analysis of MsepOR13 in the Oriental Armyworm Mythimna separata (Walker) |
| title_sort | functional analysis of msepor13 in the oriental armyworm mythimna separata walker |
| topic | Mythimna separata odorant receptor eugenol Xenopus oocytes odorant tuning |
| url | https://www.frontiersin.org/article/10.3389/fphys.2019.00367/full |
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