Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite.
Signalling pathways in malaria parasite remain poorly defined and major reason for this is the lack of understanding of the function of majority of parasite protein kinases and phosphatases in parasite signalling and its biology. In the present study, we have elucidated the function of Protein Kinas...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2023-11-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1011770&type=printable |
| _version_ | 1797200705213169664 |
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| author | Rahul Singh Rawat Ankit Gupta Neelam Antil Sonika Bhatnagar Monika Singh Akanksha Rawat T S Keshava Prasad Pushkar Sharma |
| author_facet | Rahul Singh Rawat Ankit Gupta Neelam Antil Sonika Bhatnagar Monika Singh Akanksha Rawat T S Keshava Prasad Pushkar Sharma |
| author_sort | Rahul Singh Rawat |
| collection | DOAJ |
| description | Signalling pathways in malaria parasite remain poorly defined and major reason for this is the lack of understanding of the function of majority of parasite protein kinases and phosphatases in parasite signalling and its biology. In the present study, we have elucidated the function of Protein Kinase 2 (PfPK2), which is known to be indispensable for the survival of human malaria parasite Plasmodium falciparum. We demonstrate that it is involved in the invasion of host erythrocytes, which is critical for establishing infection. In addition, PfPK2 may also be involved in the maturation of the parasite post-invasion. PfPK2 regulates the release of microneme proteins like Apical Membrane Antigen 1 (AMA1), which facilitates the formation of Tight Junction between the merozoite and host erythrocyte- a key step in the process of invasion. Comparative phosphoproteomics studies revealed that PfPK2 may be involved in regulation of several key proteins involved in invasion and signalling. Furthermore, PfPK2 regulates the generation of cGMP and the release of calcium in the parasite, which are key second messengers for the process of invasion. These and other studies have shed light on a novel signalling pathway in which PfPK2 acts as an upstream regulator of important cGMP-calcium signalling, which plays an important role in parasite invasion. |
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| format | Article |
| id | doaj.art-0b0a2b4d01d14a88b3e50eca0d876cba |
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| issn | 1553-7366 1553-7374 |
| language | English |
| last_indexed | 2024-04-24T07:35:53Z |
| publishDate | 2023-11-01 |
| publisher | Public Library of Science (PLoS) |
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| series | PLoS Pathogens |
| spelling | doaj.art-0b0a2b4d01d14a88b3e50eca0d876cba2024-04-20T05:31:19ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742023-11-011911e101177010.1371/journal.ppat.1011770Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite.Rahul Singh RawatAnkit GuptaNeelam AntilSonika BhatnagarMonika SinghAkanksha RawatT S Keshava PrasadPushkar SharmaSignalling pathways in malaria parasite remain poorly defined and major reason for this is the lack of understanding of the function of majority of parasite protein kinases and phosphatases in parasite signalling and its biology. In the present study, we have elucidated the function of Protein Kinase 2 (PfPK2), which is known to be indispensable for the survival of human malaria parasite Plasmodium falciparum. We demonstrate that it is involved in the invasion of host erythrocytes, which is critical for establishing infection. In addition, PfPK2 may also be involved in the maturation of the parasite post-invasion. PfPK2 regulates the release of microneme proteins like Apical Membrane Antigen 1 (AMA1), which facilitates the formation of Tight Junction between the merozoite and host erythrocyte- a key step in the process of invasion. Comparative phosphoproteomics studies revealed that PfPK2 may be involved in regulation of several key proteins involved in invasion and signalling. Furthermore, PfPK2 regulates the generation of cGMP and the release of calcium in the parasite, which are key second messengers for the process of invasion. These and other studies have shed light on a novel signalling pathway in which PfPK2 acts as an upstream regulator of important cGMP-calcium signalling, which plays an important role in parasite invasion.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1011770&type=printable |
| spellingShingle | Rahul Singh Rawat Ankit Gupta Neelam Antil Sonika Bhatnagar Monika Singh Akanksha Rawat T S Keshava Prasad Pushkar Sharma Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite. PLoS Pathogens |
| title | Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite. |
| title_full | Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite. |
| title_fullStr | Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite. |
| title_full_unstemmed | Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite. |
| title_short | Protein kinase PfPK2 mediated signalling is critical for host erythrocyte invasion by malaria parasite. |
| title_sort | protein kinase pfpk2 mediated signalling is critical for host erythrocyte invasion by malaria parasite |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1011770&type=printable |
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