The effect of caffeine on osteoblast proliferation after tooth extraction in Wistar rats
<span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Caffeine is the most well-known substance which consumed by most people daily. Behind its popularity as favorable drinks and </em><span style="f...
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| Format: | Article |
| Language: | English |
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Universitas Airlangga
2006-03-01
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| Series: | Dental Journal |
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| Online Access: | http://e-journal.unair.ac.id/index.php/MKG/article/view/1111 |
| Summary: | <span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>Caffeine is the most well-known substance which consumed by most people daily. Behind its popularity as favorable drinks and </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>food, this substance also known can inhibit the post extraction wound healing by decreasing the proliferation of osteoblast cells </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>through the increase of intracellular cyclic Adenosine Mono Phosphate (cAMP). The objective of this study was done to observe the </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>effect of caffeine intake toward the number of osteoblast cells during the wound healing of post dental extraction in Wistar’s rats. </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>This study was an experimental laboratory research and the post test-only control group design was used for the statistical evaluation. </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>The samples used were 24 healthy 3 months old male Wistar’s rats, with approximately 200 grams of body weight and devided into </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>4 groups. Three groups were taken and represented as a treated group (P) and the rest of one group was used as a control group </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>(KO). Caffeine diet with a dosage of 3.78 mg/100 ml grams of body weight/cc was given for 7 days in group P1, P2 for 14 days, and </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>21 days in group P3 and the diet was given orally using an oral sonde. Teeth extractions of the right first molar in the lower jaw were </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>done in all groups according to the interval time had been scheduled. Seven days of post-extraction time was waiting in all groups </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>before the sample being decapitated for further histological examination in the post extracted sites. A Hematoxillin and Eosin </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>staining was used and the number of osteoblast cells were counted under light microscopy with 400 times magnification. One-way </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>ANOVA and Least Significant Difference (LSD) test were used for the statistical evaluation. The result of the study shown a significant </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>decrease of the number of osteoblast cells in caffeine consumed group of 7, 14, and 21 days observed (p < 0.05). This study conclude </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>that the duration time of caffeine consumed had been interfered significantly with the osteoblast cell proliferation during the wound </em><span style="font-family: TribuneItalic; font-size: 9pt; color: #231f20; font-style: normal; font-variant: normal;"><em>healing after teeth extractions in Wistar’s rats.</em></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span></span> |
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| ISSN: | 1978-3728 2442-9740 |