Lifelong dietary omega-3 fatty acid suppresses thrombotic potential through gut microbiota alteration in aged mice
Summary: Aging is a major risk factor for cardiovascular diseases, including thrombotic events. The gut microbiota has been implicated in the development of thrombotic risk. Plant-derived omega-3 fatty acid ɑ-linolenic acid (ALA) confers beneficial anti-platelet and anti-inflammatory effects. Hence,...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-08-01
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| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004221008658 |
| _version_ | 1818435325959077888 |
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| author | Seyed Soheil Saeedi Saravi Nicole R. Bonetti Benoit Pugin Florentin Constancias Lisa Pasterk Sara Gobbato Alexander Akhmedov Luca Liberale Thomas F. Lüscher Giovanni G. Camici Jürg H. Beer |
| author_facet | Seyed Soheil Saeedi Saravi Nicole R. Bonetti Benoit Pugin Florentin Constancias Lisa Pasterk Sara Gobbato Alexander Akhmedov Luca Liberale Thomas F. Lüscher Giovanni G. Camici Jürg H. Beer |
| author_sort | Seyed Soheil Saeedi Saravi |
| collection | DOAJ |
| description | Summary: Aging is a major risk factor for cardiovascular diseases, including thrombotic events. The gut microbiota has been implicated in the development of thrombotic risk. Plant-derived omega-3 fatty acid ɑ-linolenic acid (ALA) confers beneficial anti-platelet and anti-inflammatory effects. Hence, antithrombotic activity elicited by ALA may be partly dependent on its interaction with gut microbiota during aging. Here, we demonstrate that lifelong dietary ALA decreases platelet hyperresponsiveness and thrombus formation in aged mice. These phenotypic changes can be partly attributed to alteration of microbial composition and reduction of its metabolite trimethylamine N-oxide and inflammatory mediators including TNF-α, as well as the upregulated production of short-chain fatty acid acetate. ALA-rich diet also dampens secretion of increased procoagulant factors, tissue factor and plasminogen activator inhibitor-1, in aged mice. Our results suggest long-term ALA supplementation as an attractive, accessible, and well-tolerated nutritional strategy against age-associated platelet hyperreactivity and thrombotic potential. |
| first_indexed | 2024-12-14T16:51:06Z |
| format | Article |
| id | doaj.art-0b0cc24c969f4fcfadbb9964be41358f |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-12-14T16:51:06Z |
| publishDate | 2021-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-0b0cc24c969f4fcfadbb9964be41358f2022-12-21T22:54:03ZengElsevieriScience2589-00422021-08-01248102897Lifelong dietary omega-3 fatty acid suppresses thrombotic potential through gut microbiota alteration in aged miceSeyed Soheil Saeedi Saravi0Nicole R. Bonetti1Benoit Pugin2Florentin Constancias3Lisa Pasterk4Sara Gobbato5Alexander Akhmedov6Luca Liberale7Thomas F. Lüscher8Giovanni G. Camici9Jürg H. Beer10Laboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, 8952 Schlieren, Switzerland; Department of Internal Medicine, Cantonal Hospital Baden, Im Ergel 1, 5404 Baden, SwitzerlandLaboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, 8952 Schlieren, Switzerland; Department of Internal Medicine, Cantonal Hospital Baden, Im Ergel 1, 5404 Baden, SwitzerlandLaboratory of Food Biotechnology, Institute of Food, Nutrition and Health, Department of Health Sciences and Technology, ETH Zurich, 8092 Zurich, SwitzerlandLaboratory of Food Biotechnology, Institute of Food, Nutrition and Health, Department of Health Sciences and Technology, ETH Zurich, 8092 Zurich, SwitzerlandLaboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, 8952 Schlieren, SwitzerlandDepartment of Internal Medicine, Cantonal Hospital Baden, Im Ergel 1, 5404 Baden, SwitzerlandCenter for Molecular Cardiology, University of Zurich, 8952 Schlieren, SwitzerlandCenter for Molecular Cardiology, University of Zurich, 8952 Schlieren, Switzerland; Department of Internal Medicine, University of Genoa, Genoa, ItalyCenter for Molecular Cardiology, University of Zurich, 8952 Schlieren, Switzerland; Royal Brompton and Harefield Hospitals and Imperial College, London, UKCenter for Molecular Cardiology, University of Zurich, 8952 Schlieren, Switzerland; University Heart Center, Department of Cardiology, University Hospital Zurich, Zurich, Switzerland; Department of Research and Education, University Hospital Zurich, Zurich, SwitzerlandLaboratory for Platelet Research, Center for Molecular Cardiology, University of Zurich, 8952 Schlieren, Switzerland; Department of Internal Medicine, Cantonal Hospital Baden, Im Ergel 1, 5404 Baden, Switzerland; Corresponding authorSummary: Aging is a major risk factor for cardiovascular diseases, including thrombotic events. The gut microbiota has been implicated in the development of thrombotic risk. Plant-derived omega-3 fatty acid ɑ-linolenic acid (ALA) confers beneficial anti-platelet and anti-inflammatory effects. Hence, antithrombotic activity elicited by ALA may be partly dependent on its interaction with gut microbiota during aging. Here, we demonstrate that lifelong dietary ALA decreases platelet hyperresponsiveness and thrombus formation in aged mice. These phenotypic changes can be partly attributed to alteration of microbial composition and reduction of its metabolite trimethylamine N-oxide and inflammatory mediators including TNF-α, as well as the upregulated production of short-chain fatty acid acetate. ALA-rich diet also dampens secretion of increased procoagulant factors, tissue factor and plasminogen activator inhibitor-1, in aged mice. Our results suggest long-term ALA supplementation as an attractive, accessible, and well-tolerated nutritional strategy against age-associated platelet hyperreactivity and thrombotic potential.http://www.sciencedirect.com/science/article/pii/S2589004221008658HematologyNutritionMicrobiome |
| spellingShingle | Seyed Soheil Saeedi Saravi Nicole R. Bonetti Benoit Pugin Florentin Constancias Lisa Pasterk Sara Gobbato Alexander Akhmedov Luca Liberale Thomas F. Lüscher Giovanni G. Camici Jürg H. Beer Lifelong dietary omega-3 fatty acid suppresses thrombotic potential through gut microbiota alteration in aged mice iScience Hematology Nutrition Microbiome |
| title | Lifelong dietary omega-3 fatty acid suppresses thrombotic potential through gut microbiota alteration in aged mice |
| title_full | Lifelong dietary omega-3 fatty acid suppresses thrombotic potential through gut microbiota alteration in aged mice |
| title_fullStr | Lifelong dietary omega-3 fatty acid suppresses thrombotic potential through gut microbiota alteration in aged mice |
| title_full_unstemmed | Lifelong dietary omega-3 fatty acid suppresses thrombotic potential through gut microbiota alteration in aged mice |
| title_short | Lifelong dietary omega-3 fatty acid suppresses thrombotic potential through gut microbiota alteration in aged mice |
| title_sort | lifelong dietary omega 3 fatty acid suppresses thrombotic potential through gut microbiota alteration in aged mice |
| topic | Hematology Nutrition Microbiome |
| url | http://www.sciencedirect.com/science/article/pii/S2589004221008658 |
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