The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots
Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and t...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-09-01
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| Series: | Stem Cell Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506116300988 |
| _version_ | 1819259433093431296 |
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| author | Camilla Siciliano Antonella Bordin Mohsen Ibrahim Isotta Chimenti Francesco Cassiano Ilenia Gatto Giorgio Mangino Andrea Coccia Selenia Miglietta Daniela Bastianelli Vincenzo Petrozza Antonella Calogero Giacomo Frati Elena De Falco |
| author_facet | Camilla Siciliano Antonella Bordin Mohsen Ibrahim Isotta Chimenti Francesco Cassiano Ilenia Gatto Giorgio Mangino Andrea Coccia Selenia Miglietta Daniela Bastianelli Vincenzo Petrozza Antonella Calogero Giacomo Frati Elena De Falco |
| author_sort | Camilla Siciliano |
| collection | DOAJ |
| description | Indirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed. |
| first_indexed | 2024-12-23T19:09:56Z |
| format | Article |
| id | doaj.art-0b192e007b01496d9457cba6e4784633 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 1876-7753 |
| language | English |
| last_indexed | 2024-12-23T19:09:56Z |
| publishDate | 2016-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-0b192e007b01496d9457cba6e47846332022-12-21T17:34:30ZengElsevierStem Cell Research1873-50611876-77532016-09-0117234235110.1016/j.scr.2016.07.010The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depotsCamilla Siciliano0Antonella Bordin1Mohsen Ibrahim2Isotta Chimenti3Francesco Cassiano4Ilenia Gatto5Giorgio Mangino6Andrea Coccia7Selenia Miglietta8Daniela Bastianelli9Vincenzo Petrozza10Antonella Calogero11Giacomo Frati12Elena De Falco13Department of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDivision of Thoracic Surgery, Department of Medical-Surgical Science and Translational Medicine, Sapienza University of Rome, S. Andrea Hospital, via di Grottarossa 1035, 00189 Rome, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDepartment of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory “Pietro M. Motta”, Sapienza University of Rome, Rome, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDepartment of Anatomy, Histology, Forensic Medicine and Orthopaedics, Section of Anatomy, Electron Microscopy Unit, Laboratory “Pietro M. Motta”, Sapienza University of Rome, Rome, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyDepartment of Medical-Surgical Sciences and Biotechnologies, Sapienza University of Rome Polo Pontino, C.so della Repubblica 79, 04100 Latina, ItalyIndirect evidence suggests that adipose tissue-derived stromal cells (ASCs) possess different physiological and biological variations related to the anatomical localization of the adipose depots. Accordingly, to investigate the influence of the tissue origin on the intrinsic properties of ASCs and to assess their response to specific stimuli, we compared the biological, functional and ultrastructural properties of two ASC pools derived from mediastinal and subcutaneous depots (thoracic compartment) by means of supplements such as platelet lysate (PL) and FBS. Subcutaneous ASCs exhibited higher proliferative and clonogenic abilities than mediastinal counterpart, as well as increased secreted levels of IL-6 combined with lower amount of VEGF-C. In contrast, mediastinal ASCs displayed enhanced pro-angiogenic and adipogenic differentiation properties, increased cell diameter and early autophagic processes, highlighted by electron microscopy. Our results further support the hypothesis that the origin of adipose tissue significantly defines the biological properties of ASCs, and that a homogeneric function for all ASCs cannot be assumed.http://www.sciencedirect.com/science/article/pii/S1873506116300988Mesenchymal stem cellsAdipose tissuePlatelet lysate angiogenesisTransmission electron microscopy |
| spellingShingle | Camilla Siciliano Antonella Bordin Mohsen Ibrahim Isotta Chimenti Francesco Cassiano Ilenia Gatto Giorgio Mangino Andrea Coccia Selenia Miglietta Daniela Bastianelli Vincenzo Petrozza Antonella Calogero Giacomo Frati Elena De Falco The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots Stem Cell Research Mesenchymal stem cells Adipose tissue Platelet lysate angiogenesis Transmission electron microscopy |
| title | The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots |
| title_full | The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots |
| title_fullStr | The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots |
| title_full_unstemmed | The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots |
| title_short | The adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots |
| title_sort | adipose tissue of origin influences the biological potential of human adipose stromal cells isolated from mediastinal and subcutaneous fat depots |
| topic | Mesenchymal stem cells Adipose tissue Platelet lysate angiogenesis Transmission electron microscopy |
| url | http://www.sciencedirect.com/science/article/pii/S1873506116300988 |
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