High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells
Summary: Investigation of human muscle regeneration requires robust methods to purify and transplant muscle stem and progenitor cells that collectively constitute the human satellite cell (HuSC) pool. Existing approaches have yet to make HuSCs widely accessible for researchers, and as a result human...
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2018-03-01
|
| Series: | Stem Cell Reports |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213671118300481 |
| _version_ | 1818570965316009984 |
|---|---|
| author | Steven M. Garcia Stanley Tamaki Solomon Lee Alvin Wong Anthony Jose Joanna Dreux Gayle Kouklis Hani Sbitany Rahul Seth P. Daniel Knott Chase Heaton William R. Ryan Esther A. Kim Scott L. Hansen William Y. Hoffman Jason H. Pomerantz |
| author_facet | Steven M. Garcia Stanley Tamaki Solomon Lee Alvin Wong Anthony Jose Joanna Dreux Gayle Kouklis Hani Sbitany Rahul Seth P. Daniel Knott Chase Heaton William R. Ryan Esther A. Kim Scott L. Hansen William Y. Hoffman Jason H. Pomerantz |
| author_sort | Steven M. Garcia |
| collection | DOAJ |
| description | Summary: Investigation of human muscle regeneration requires robust methods to purify and transplant muscle stem and progenitor cells that collectively constitute the human satellite cell (HuSC) pool. Existing approaches have yet to make HuSCs widely accessible for researchers, and as a result human muscle stem cell research has advanced slowly. Here, we describe a robust and predictable HuSC purification process that is effective for each human skeletal muscle tested and the development of storage protocols and transplantation models in dystrophin-deficient and wild-type recipients. Enzymatic digestion, magnetic column depletion, and 6-marker flow-cytometric purification enable separation of 104 highly enriched HuSCs per gram of muscle. Cryostorage of HuSCs preserves viability, phenotype, and transplantation potential. Development of enhanced and species-specific transplantation protocols enabled serial HuSC xenotransplantation and recovery. These protocols and models provide an accessible system for basic and translational investigation and clinical development of HuSCs. : Garcia and colleagues report methods for efficient purification of satellite cells from human skeletal muscle. They use their approaches to demonstrate stem cell functions of endogenous satellite cells and to make human satellite cells accessible for sharing among researchers. Keywords: human satellite cell purification, serial transplantation, satellite cell cryopreservation |
| first_indexed | 2024-12-14T13:49:17Z |
| format | Article |
| id | doaj.art-0b1976f0cb514dd788e72aaa6454802e |
| institution | Directory Open Access Journal |
| issn | 2213-6711 |
| language | English |
| last_indexed | 2024-12-14T13:49:17Z |
| publishDate | 2018-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Reports |
| spelling | doaj.art-0b1976f0cb514dd788e72aaa6454802e2022-12-21T22:59:11ZengElsevierStem Cell Reports2213-67112018-03-0110311601174High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite CellsSteven M. Garcia0Stanley Tamaki1Solomon Lee2Alvin Wong3Anthony Jose4Joanna Dreux5Gayle Kouklis6Hani Sbitany7Rahul Seth8P. Daniel Knott9Chase Heaton10William R. Ryan11Esther A. Kim12Scott L. Hansen13William Y. Hoffman14Jason H. Pomerantz15Department of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Otolaryngology - Head and Neck Surgery, University of California, San Francisco, CA 94143, USADepartment of Otolaryngology - Head and Neck Surgery, University of California, San Francisco, CA 94143, USADepartment of Otolaryngology - Head and Neck Surgery, University of California, San Francisco, CA 94143, USADepartment of Otolaryngology - Head and Neck Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartment of Surgery, Division of Plastic and Reconstructive Surgery, University of California, San Francisco, CA 94143, USADepartments of Surgery and Orofacial Sciences, Division of Plastic and Reconstructive Surgery, Program in Craniofacial Biology, Eli and Edythe Broad Center of Regeneration Medicine, University of California, San Francisco, CA 94143, USA; Corresponding authorSummary: Investigation of human muscle regeneration requires robust methods to purify and transplant muscle stem and progenitor cells that collectively constitute the human satellite cell (HuSC) pool. Existing approaches have yet to make HuSCs widely accessible for researchers, and as a result human muscle stem cell research has advanced slowly. Here, we describe a robust and predictable HuSC purification process that is effective for each human skeletal muscle tested and the development of storage protocols and transplantation models in dystrophin-deficient and wild-type recipients. Enzymatic digestion, magnetic column depletion, and 6-marker flow-cytometric purification enable separation of 104 highly enriched HuSCs per gram of muscle. Cryostorage of HuSCs preserves viability, phenotype, and transplantation potential. Development of enhanced and species-specific transplantation protocols enabled serial HuSC xenotransplantation and recovery. These protocols and models provide an accessible system for basic and translational investigation and clinical development of HuSCs. : Garcia and colleagues report methods for efficient purification of satellite cells from human skeletal muscle. They use their approaches to demonstrate stem cell functions of endogenous satellite cells and to make human satellite cells accessible for sharing among researchers. Keywords: human satellite cell purification, serial transplantation, satellite cell cryopreservationhttp://www.sciencedirect.com/science/article/pii/S2213671118300481 |
| spellingShingle | Steven M. Garcia Stanley Tamaki Solomon Lee Alvin Wong Anthony Jose Joanna Dreux Gayle Kouklis Hani Sbitany Rahul Seth P. Daniel Knott Chase Heaton William R. Ryan Esther A. Kim Scott L. Hansen William Y. Hoffman Jason H. Pomerantz High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells Stem Cell Reports |
| title | High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells |
| title_full | High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells |
| title_fullStr | High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells |
| title_full_unstemmed | High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells |
| title_short | High-Yield Purification, Preservation, and Serial Transplantation of Human Satellite Cells |
| title_sort | high yield purification preservation and serial transplantation of human satellite cells |
| url | http://www.sciencedirect.com/science/article/pii/S2213671118300481 |
| work_keys_str_mv | AT stevenmgarcia highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT stanleytamaki highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT solomonlee highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT alvinwong highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT anthonyjose highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT joannadreux highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT gaylekouklis highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT hanisbitany highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT rahulseth highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT pdanielknott highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT chaseheaton highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT williamrryan highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT estherakim highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT scottlhansen highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT williamyhoffman highyieldpurificationpreservationandserialtransplantationofhumansatellitecells AT jasonhpomerantz highyieldpurificationpreservationandserialtransplantationofhumansatellitecells |