Urinary 8-hydroxydeoxyguanosine in relation to XRCC1 rs25487 G/A (Arg399Gln) and OGG1 rs1052133 C/G (Ser326Cys) DNA repair genes polymorphisms in patients with chronic hepatitis C and related hepatocellular carcinoma
Aida A Mahmoud,1 Mohammed H Hassan,2 Ali A Ghweil,3 Amany Abdelrahman,4 Asmaa N Mohammad,5 Hesham H Ameen61Medical Biochemistry Department, Faculty of Medicine, Sohag University, Sohag, Egypt; 2Medical Biochemistry Department, Faculty of Medicine, South Valley University, Qena, Egypt; 3Tropical Medi...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2019-06-01
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| Series: | Cancer Management and Research |
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| Online Access: | https://www.dovepress.com/urinary-8-hydroxydeoxyguanosine-in-relation-to-xrcc1-rs25487-ga-arg399-peer-reviewed-article-CMAR |
| _version_ | 1828863911810564096 |
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| author | Mahmoud AA Hassan MH Ghweil AA Abdelrahman A Mohammad AN Ameen HH |
| author_facet | Mahmoud AA Hassan MH Ghweil AA Abdelrahman A Mohammad AN Ameen HH |
| author_sort | Mahmoud AA |
| collection | DOAJ |
| description | Aida A Mahmoud,1 Mohammed H Hassan,2 Ali A Ghweil,3 Amany Abdelrahman,4 Asmaa N Mohammad,5 Hesham H Ameen61Medical Biochemistry Department, Faculty of Medicine, Sohag University, Sohag, Egypt; 2Medical Biochemistry Department, Faculty of Medicine, South Valley University, Qena, Egypt; 3Tropical Medicine and Gastroenterology Department, Faculty of Medicine, South Valley University, Qena, Egypt; 4Medical Physiology Department, Faculty of Medicine, Sohag University, Sohag, Egypt; 5Tropical Medicine and Gastroenterology Department, Faculty of Medicine, Sohag University, Sohag, Egypt; 6Clinical Pathology, Faculty of Medicine, Al-Azhar University (Assiut Branch), Assiut, EgyptBackground and aim: DNA repair represents a protective mechanism against cell injury and cancer. 8-hydroxy-deoxyguanosine (8-OHdG) is the main ROS-induced DNA mutation. The current study aimed to evaluate urinary 8-OHdG levels in patients with chronic hepatitis C virus (HCV) and its related hepatocellular (HCC) and correlate its level to XRCC1 rs25487 G/A and OGG1 rs1052133 C/G gene polymorphisms.Materials and methods: Urinary 8-OHdG assays were performed using HPLC technique, and XRCC1 rs25487 G/A and OGG1 rs1052133 C/G gene polymorphisms were analyzed by PCR using confronting two-pair primer method (PCR-CTPP) in 200 subjects allocated into 50 chronic HCV patients, 50 HCV-related HCC patients, and 100 controls.Results: There were significantly increased urinary 8-OHdG levels in HCV-related HCC and chronic HCV patients when compared with the controls (P<0.05 for all). Urinary 8-OHdG was associated with the tumor spread. Regarding, XRCC1 (Arg399Gln), AA (Gln/Gln) genotype and A-allele were more frequent in HCC and chronic HCV patients than in the controls (P<0.05). ORs (95%CI) using the dominant and the recessive genetic models were; 2.1 (1.1–4.1), P=0.032 and 1.9 (1–3.6), P=0.043 respectively. For OGG1 (Ser326Cys), GG (Cys/Cys) genotype and G-allele were increased significantly in chronic HCV and HCC patients compared to the controls (P<0.05). ORs (95%CI) under the dominant and the recessive genetic models were; 2.1 (1.1–4.1), P=0.032 and 1.9 (1–3.8), P=0.049 respectively. Additionally, XRCC1 (AA) and OGG1 (GG) genotypes had significantly increased urinary 8-OHdG levels among patients (P<0.05).Conclusions: XRCC1 (AA) and OGG1 (GG) could be considered as possible genotypic risk factors for HCV- related HCC development which were associated with significantly high urinary 8-hydroxy-deoxyguanosine levels, thus urinary 8-OHdG could be considered as non-invasive marker in follow-up chronic HCV progression into HCC.Keywords: urinary 8-hydroxy-deoxyguanosine, high performance liquid chromatography, confronting two-pair primers, XRCC1 and OGG1 SNPs, DNA repair, hepatocellular carcinoma |
| first_indexed | 2024-12-13T03:54:07Z |
| format | Article |
| id | doaj.art-0b1bf8c684044d8dbab7c6a5b45b732f |
| institution | Directory Open Access Journal |
| issn | 1179-1322 |
| language | English |
| last_indexed | 2024-12-13T03:54:07Z |
| publishDate | 2019-06-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Cancer Management and Research |
| spelling | doaj.art-0b1bf8c684044d8dbab7c6a5b45b732f2022-12-22T00:00:39ZengDove Medical PressCancer Management and Research1179-13222019-06-01Volume 115343535146379Urinary 8-hydroxydeoxyguanosine in relation to XRCC1 rs25487 G/A (Arg399Gln) and OGG1 rs1052133 C/G (Ser326Cys) DNA repair genes polymorphisms in patients with chronic hepatitis C and related hepatocellular carcinomaMahmoud AAHassan MHGhweil AAAbdelrahman AMohammad ANAmeen HHAida A Mahmoud,1 Mohammed H Hassan,2 Ali A Ghweil,3 Amany Abdelrahman,4 Asmaa N Mohammad,5 Hesham H Ameen61Medical Biochemistry Department, Faculty of Medicine, Sohag University, Sohag, Egypt; 2Medical Biochemistry Department, Faculty of Medicine, South Valley University, Qena, Egypt; 3Tropical Medicine and Gastroenterology Department, Faculty of Medicine, South Valley University, Qena, Egypt; 4Medical Physiology Department, Faculty of Medicine, Sohag University, Sohag, Egypt; 5Tropical Medicine and Gastroenterology Department, Faculty of Medicine, Sohag University, Sohag, Egypt; 6Clinical Pathology, Faculty of Medicine, Al-Azhar University (Assiut Branch), Assiut, EgyptBackground and aim: DNA repair represents a protective mechanism against cell injury and cancer. 8-hydroxy-deoxyguanosine (8-OHdG) is the main ROS-induced DNA mutation. The current study aimed to evaluate urinary 8-OHdG levels in patients with chronic hepatitis C virus (HCV) and its related hepatocellular (HCC) and correlate its level to XRCC1 rs25487 G/A and OGG1 rs1052133 C/G gene polymorphisms.Materials and methods: Urinary 8-OHdG assays were performed using HPLC technique, and XRCC1 rs25487 G/A and OGG1 rs1052133 C/G gene polymorphisms were analyzed by PCR using confronting two-pair primer method (PCR-CTPP) in 200 subjects allocated into 50 chronic HCV patients, 50 HCV-related HCC patients, and 100 controls.Results: There were significantly increased urinary 8-OHdG levels in HCV-related HCC and chronic HCV patients when compared with the controls (P<0.05 for all). Urinary 8-OHdG was associated with the tumor spread. Regarding, XRCC1 (Arg399Gln), AA (Gln/Gln) genotype and A-allele were more frequent in HCC and chronic HCV patients than in the controls (P<0.05). ORs (95%CI) using the dominant and the recessive genetic models were; 2.1 (1.1–4.1), P=0.032 and 1.9 (1–3.6), P=0.043 respectively. For OGG1 (Ser326Cys), GG (Cys/Cys) genotype and G-allele were increased significantly in chronic HCV and HCC patients compared to the controls (P<0.05). ORs (95%CI) under the dominant and the recessive genetic models were; 2.1 (1.1–4.1), P=0.032 and 1.9 (1–3.8), P=0.049 respectively. Additionally, XRCC1 (AA) and OGG1 (GG) genotypes had significantly increased urinary 8-OHdG levels among patients (P<0.05).Conclusions: XRCC1 (AA) and OGG1 (GG) could be considered as possible genotypic risk factors for HCV- related HCC development which were associated with significantly high urinary 8-hydroxy-deoxyguanosine levels, thus urinary 8-OHdG could be considered as non-invasive marker in follow-up chronic HCV progression into HCC.Keywords: urinary 8-hydroxy-deoxyguanosine, high performance liquid chromatography, confronting two-pair primers, XRCC1 and OGG1 SNPs, DNA repair, hepatocellular carcinomahttps://www.dovepress.com/urinary-8-hydroxydeoxyguanosine-in-relation-to-xrcc1-rs25487-ga-arg399-peer-reviewed-article-CMARUrinary 8-hydroxy-deoxyguanosineHigh performance liquid chromatographyConfronting two-pair primersXRCC1 and OGG1 SNPsDNA repairHepatocellular carcinoma. |
| spellingShingle | Mahmoud AA Hassan MH Ghweil AA Abdelrahman A Mohammad AN Ameen HH Urinary 8-hydroxydeoxyguanosine in relation to XRCC1 rs25487 G/A (Arg399Gln) and OGG1 rs1052133 C/G (Ser326Cys) DNA repair genes polymorphisms in patients with chronic hepatitis C and related hepatocellular carcinoma Cancer Management and Research Urinary 8-hydroxy-deoxyguanosine High performance liquid chromatography Confronting two-pair primers XRCC1 and OGG1 SNPs DNA repair Hepatocellular carcinoma. |
| title | Urinary 8-hydroxydeoxyguanosine in relation to XRCC1 rs25487 G/A (Arg399Gln) and OGG1 rs1052133 C/G (Ser326Cys) DNA repair genes polymorphisms in patients with chronic hepatitis C and related hepatocellular carcinoma |
| title_full | Urinary 8-hydroxydeoxyguanosine in relation to XRCC1 rs25487 G/A (Arg399Gln) and OGG1 rs1052133 C/G (Ser326Cys) DNA repair genes polymorphisms in patients with chronic hepatitis C and related hepatocellular carcinoma |
| title_fullStr | Urinary 8-hydroxydeoxyguanosine in relation to XRCC1 rs25487 G/A (Arg399Gln) and OGG1 rs1052133 C/G (Ser326Cys) DNA repair genes polymorphisms in patients with chronic hepatitis C and related hepatocellular carcinoma |
| title_full_unstemmed | Urinary 8-hydroxydeoxyguanosine in relation to XRCC1 rs25487 G/A (Arg399Gln) and OGG1 rs1052133 C/G (Ser326Cys) DNA repair genes polymorphisms in patients with chronic hepatitis C and related hepatocellular carcinoma |
| title_short | Urinary 8-hydroxydeoxyguanosine in relation to XRCC1 rs25487 G/A (Arg399Gln) and OGG1 rs1052133 C/G (Ser326Cys) DNA repair genes polymorphisms in patients with chronic hepatitis C and related hepatocellular carcinoma |
| title_sort | urinary 8 hydroxydeoxyguanosine in relation to xrcc1 rs25487 g a arg399gln and ogg1 rs1052133 c g ser326cys dna repair genes polymorphisms in patients with chronic hepatitis c and related hepatocellular carcinoma |
| topic | Urinary 8-hydroxy-deoxyguanosine High performance liquid chromatography Confronting two-pair primers XRCC1 and OGG1 SNPs DNA repair Hepatocellular carcinoma. |
| url | https://www.dovepress.com/urinary-8-hydroxydeoxyguanosine-in-relation-to-xrcc1-rs25487-ga-arg399-peer-reviewed-article-CMAR |
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