Welding Fume Instillation in Isolated Perfused Mouse Lungs—Effects of Zinc- and Copper-Containing Welding Fumes
Zinc- and copper-containing welding fumes can cause systemic inflammation after exposure in humans. Recent ex vivo studies have shown that the observed inflammation originates from exposed immune cells. In vitro studies identified the soluble fraction of metal particles as the main effectors. Isolat...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-08-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/16/9052 |
| _version_ | 1797409786868793344 |
|---|---|
| author | Julia Krabbe Thomas Kraus Hanif Krabbe Christian Martin Patrick Ziegler |
| author_facet | Julia Krabbe Thomas Kraus Hanif Krabbe Christian Martin Patrick Ziegler |
| author_sort | Julia Krabbe |
| collection | DOAJ |
| description | Zinc- and copper-containing welding fumes can cause systemic inflammation after exposure in humans. Recent ex vivo studies have shown that the observed inflammation originates from exposed immune cells. In vitro studies identified the soluble fraction of metal particles as the main effectors. Isolated perfused mouse lungs (IPLs) were perfused and ventilated for 270 min. Lungs were instilled with saline solution (control), welding fume particle suspension (WFs) or the soluble fraction of the welding fumes (SF-WFs). Bronchoalveolar lavage fluid (BALF) and perfusate samples were analyzed for cytokine levels and lung tissue mRNA expression levels were analyzed via RT-PCR. All lungs instilled with WFs did not complete the experiments due to a fatal reduction in tidal volume. Accordingly, IL-6 and MPO levels were significantly higher in BALF of WF lungs compared to the control. IL-6 and MPO mRNA expression levels were also increased for WFs. Lungs instilled with SF-WFs only showed mild reactions in tidal volume, with BALF and mRNA expression levels not significantly differing from the control. Zinc- and copper-containing welding fume particles adversely affect IPLs when instilled, as evidenced by the fatal loss in tidal volume and increased cytokine expression and secretion. The effects are mainly caused by the particles, not by the soluble fraction. |
| first_indexed | 2024-03-09T04:19:46Z |
| format | Article |
| id | doaj.art-0b1ca13a1ee7429f9cd0a7ef9cebdc76 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T04:19:46Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-0b1ca13a1ee7429f9cd0a7ef9cebdc762023-12-03T13:48:15ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-08-012316905210.3390/ijms23169052Welding Fume Instillation in Isolated Perfused Mouse Lungs—Effects of Zinc- and Copper-Containing Welding FumesJulia Krabbe0Thomas Kraus1Hanif Krabbe2Christian Martin3Patrick Ziegler4Institute of Occupational, Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstraße 30, 52074 Aachen, GermanyInstitute of Occupational, Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstraße 30, 52074 Aachen, GermanyEuropean Vascular Centre Aachen-Maastricht, Department of Vascular Surgery, Medical Faculty, University Hospital RWTH Aachen, Pauwelsstraße 30, 52074 Aachen, GermanyInstitute of Pharmacology and Toxicology, Medical Faculty, RWTH Aachen University, Wendlingweg 2, 52074 Aachen, GermanyInstitute of Occupational, Social and Environmental Medicine, Medical Faculty, RWTH Aachen University, Pauwelsstraße 30, 52074 Aachen, GermanyZinc- and copper-containing welding fumes can cause systemic inflammation after exposure in humans. Recent ex vivo studies have shown that the observed inflammation originates from exposed immune cells. In vitro studies identified the soluble fraction of metal particles as the main effectors. Isolated perfused mouse lungs (IPLs) were perfused and ventilated for 270 min. Lungs were instilled with saline solution (control), welding fume particle suspension (WFs) or the soluble fraction of the welding fumes (SF-WFs). Bronchoalveolar lavage fluid (BALF) and perfusate samples were analyzed for cytokine levels and lung tissue mRNA expression levels were analyzed via RT-PCR. All lungs instilled with WFs did not complete the experiments due to a fatal reduction in tidal volume. Accordingly, IL-6 and MPO levels were significantly higher in BALF of WF lungs compared to the control. IL-6 and MPO mRNA expression levels were also increased for WFs. Lungs instilled with SF-WFs only showed mild reactions in tidal volume, with BALF and mRNA expression levels not significantly differing from the control. Zinc- and copper-containing welding fume particles adversely affect IPLs when instilled, as evidenced by the fatal loss in tidal volume and increased cytokine expression and secretion. The effects are mainly caused by the particles, not by the soluble fraction.https://www.mdpi.com/1422-0067/23/16/9052isolated perfused lungwelding fumeszinccoppermyeloperoxidase |
| spellingShingle | Julia Krabbe Thomas Kraus Hanif Krabbe Christian Martin Patrick Ziegler Welding Fume Instillation in Isolated Perfused Mouse Lungs—Effects of Zinc- and Copper-Containing Welding Fumes International Journal of Molecular Sciences isolated perfused lung welding fumes zinc copper myeloperoxidase |
| title | Welding Fume Instillation in Isolated Perfused Mouse Lungs—Effects of Zinc- and Copper-Containing Welding Fumes |
| title_full | Welding Fume Instillation in Isolated Perfused Mouse Lungs—Effects of Zinc- and Copper-Containing Welding Fumes |
| title_fullStr | Welding Fume Instillation in Isolated Perfused Mouse Lungs—Effects of Zinc- and Copper-Containing Welding Fumes |
| title_full_unstemmed | Welding Fume Instillation in Isolated Perfused Mouse Lungs—Effects of Zinc- and Copper-Containing Welding Fumes |
| title_short | Welding Fume Instillation in Isolated Perfused Mouse Lungs—Effects of Zinc- and Copper-Containing Welding Fumes |
| title_sort | welding fume instillation in isolated perfused mouse lungs effects of zinc and copper containing welding fumes |
| topic | isolated perfused lung welding fumes zinc copper myeloperoxidase |
| url | https://www.mdpi.com/1422-0067/23/16/9052 |
| work_keys_str_mv | AT juliakrabbe weldingfumeinstillationinisolatedperfusedmouselungseffectsofzincandcoppercontainingweldingfumes AT thomaskraus weldingfumeinstillationinisolatedperfusedmouselungseffectsofzincandcoppercontainingweldingfumes AT hanifkrabbe weldingfumeinstillationinisolatedperfusedmouselungseffectsofzincandcoppercontainingweldingfumes AT christianmartin weldingfumeinstillationinisolatedperfusedmouselungseffectsofzincandcoppercontainingweldingfumes AT patrickziegler weldingfumeinstillationinisolatedperfusedmouselungseffectsofzincandcoppercontainingweldingfumes |