Protective mechanism of hydrogen on oxidative stress-induced retinal aging
AIM: To investigate the protective mechanism of hydrogen on retinal senescence induced by oxidative stress.<p>METHODS: Mice were randomly divided into three groups: control group, model group and treatment group. Animal models of retinal oxidative stress injury were established by injecting so...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Press of International Journal of Ophthalmology (IJO PRESS)
2019-02-01
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| Series: | Guoji Yanke Zazhi |
| Subjects: | |
| Online Access: | http://ies.ijo.cn/cn_publish/2019/2/201902004.pdf |
| Summary: | AIM: To investigate the protective mechanism of hydrogen on retinal senescence induced by oxidative stress.<p>METHODS: Mice were randomly divided into three groups: control group, model group and treatment group. Animal models of retinal oxidative stress injury were established by injecting sodium iodate solution into mice caudal vein. Harvesting the mice retina, Western-blot was used to detect the level of proteins related to DNA damage response such as ATM, NF-κB, cyclin D1 and HMGB1 that associated with DNA repair.<p>RESULTS: SA-β-gal staining showed that the blue-green deposits in treatment group were reduced than that in model group. The expression of DNA damage reactive protein in treatment group ATM, cyclin D1, NF-κB(0.10±0.009, 0.32±0.01, 0.19±0.002)were significantly lower than those in the model groups(0.77±0.08, 0.70±0.02, 0.36±0.01), and the differences were statistically significant(all <i>P</i><0.01). At the same time, the expression of DNA repair protein HMGB1 in treatment group(0.927±0.06)were notably higher than that in model group(0.383±0.07)and the difference was statistically significant(<i>P</i><0.01).<p>CONCLUSION: H<sub>2</sub> can attenuate senescence by inhibiting oxidative-stress induced DNA damage. |
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| ISSN: | 1672-5123 1672-5123 |