<i>Mastomys natalensis</i> Has a Cellular Immune Response Profile Distinct from Laboratory Mice
The multimammate mouse (<i>Mastomys natalensis; M. natalensis</i>) has been identified as a major reservoir for multiple human pathogens including Lassa virus (LASV), <i>Leishmania</i> spp., <i>Yersinia</i> spp., and <i>Borrelia</i> spp. Although <i...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/13/5/729 |
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| author | Tsing-Lee Tang-Huau Kyle Rosenke Kimberly Meade-White Aaron Carmody Brian J. Smith Catharine M. Bosio Michael A. Jarvis Heinz Feldmann |
| author_facet | Tsing-Lee Tang-Huau Kyle Rosenke Kimberly Meade-White Aaron Carmody Brian J. Smith Catharine M. Bosio Michael A. Jarvis Heinz Feldmann |
| author_sort | Tsing-Lee Tang-Huau |
| collection | DOAJ |
| description | The multimammate mouse (<i>Mastomys natalensis; M. natalensis</i>) has been identified as a major reservoir for multiple human pathogens including Lassa virus (LASV), <i>Leishmania</i> spp., <i>Yersinia</i> spp., and <i>Borrelia</i> spp. Although <i>M. natalensis</i> are related to well-characterized mouse and rat species commonly used in laboratory models, there is an absence of established assays and reagents to study the host immune responses of <i>M. natalensis</i>. As a result, there are major limitations to our understanding of immunopathology and mechanisms of immunological pathogen control in this increasingly important rodent species. In the current study, a large panel of commercially available rodent reagents were screened to identify their cross-reactivity with <i>M. natalensis.</i> Using these reagents, ex vivo assays were established and optimized to evaluate lymphocyte proliferation and cytokine production by <i>M. natalensis</i> lymphocytes. In contrast to C57BL/6J mice, lymphocytes from <i>M. natalensis</i> were relatively non-responsive to common stimuli such as phytohaemagglutinin P and lipopolysaccharide. However, they readily responded to concanavalin A stimulation as indicated by proliferation and cytokine production. In summary, we describe lymphoproliferative and cytokine assays demonstrating that the cellular immune responses in <i>M. natalensis</i> to commonly used mitogens differ from a laboratory-bred mouse strain. |
| first_indexed | 2024-03-10T12:04:23Z |
| format | Article |
| id | doaj.art-0b21969504754e05a751ac7d9599b0e7 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-10T12:04:23Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-0b21969504754e05a751ac7d9599b0e72023-11-21T16:42:05ZengMDPI AGViruses1999-49152021-04-0113572910.3390/v13050729<i>Mastomys natalensis</i> Has a Cellular Immune Response Profile Distinct from Laboratory MiceTsing-Lee Tang-Huau0Kyle Rosenke1Kimberly Meade-White2Aaron Carmody3Brian J. Smith4Catharine M. Bosio5Michael A. Jarvis6Heinz Feldmann7Laboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institute of Health, Hamilton, MT 59840, USALaboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institute of Health, Hamilton, MT 59840, USALaboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institute of Health, Hamilton, MT 59840, USAResearch Technologies Branch, Rocky Mountain Laboratories, NIAID, NIH, Hamilton, MT 59840, USARocky Mountain Veterinary Branch Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institute of Health, Hamilton, MT 59840, USALaboratory of Bacteriology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institute of Health, Hamilton, MT 59840, USAFaculty of Health: Medicine, Dentistry and Human Sciences, School of Biomedical Sciences, University of Plymouth, PL4 8AA, UKLaboratory of Virology, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, Rocky Mountain Laboratories, National Institute of Health, Hamilton, MT 59840, USAThe multimammate mouse (<i>Mastomys natalensis; M. natalensis</i>) has been identified as a major reservoir for multiple human pathogens including Lassa virus (LASV), <i>Leishmania</i> spp., <i>Yersinia</i> spp., and <i>Borrelia</i> spp. Although <i>M. natalensis</i> are related to well-characterized mouse and rat species commonly used in laboratory models, there is an absence of established assays and reagents to study the host immune responses of <i>M. natalensis</i>. As a result, there are major limitations to our understanding of immunopathology and mechanisms of immunological pathogen control in this increasingly important rodent species. In the current study, a large panel of commercially available rodent reagents were screened to identify their cross-reactivity with <i>M. natalensis.</i> Using these reagents, ex vivo assays were established and optimized to evaluate lymphocyte proliferation and cytokine production by <i>M. natalensis</i> lymphocytes. In contrast to C57BL/6J mice, lymphocytes from <i>M. natalensis</i> were relatively non-responsive to common stimuli such as phytohaemagglutinin P and lipopolysaccharide. However, they readily responded to concanavalin A stimulation as indicated by proliferation and cytokine production. In summary, we describe lymphoproliferative and cytokine assays demonstrating that the cellular immune responses in <i>M. natalensis</i> to commonly used mitogens differ from a laboratory-bred mouse strain.https://www.mdpi.com/1999-4915/13/5/729<i>Mastomys natalensis</i>immune responseT celleffector cytokinesconcanavalin Aphytohaemagglutinin P |
| spellingShingle | Tsing-Lee Tang-Huau Kyle Rosenke Kimberly Meade-White Aaron Carmody Brian J. Smith Catharine M. Bosio Michael A. Jarvis Heinz Feldmann <i>Mastomys natalensis</i> Has a Cellular Immune Response Profile Distinct from Laboratory Mice Viruses <i>Mastomys natalensis</i> immune response T cell effector cytokines concanavalin A phytohaemagglutinin P |
| title | <i>Mastomys natalensis</i> Has a Cellular Immune Response Profile Distinct from Laboratory Mice |
| title_full | <i>Mastomys natalensis</i> Has a Cellular Immune Response Profile Distinct from Laboratory Mice |
| title_fullStr | <i>Mastomys natalensis</i> Has a Cellular Immune Response Profile Distinct from Laboratory Mice |
| title_full_unstemmed | <i>Mastomys natalensis</i> Has a Cellular Immune Response Profile Distinct from Laboratory Mice |
| title_short | <i>Mastomys natalensis</i> Has a Cellular Immune Response Profile Distinct from Laboratory Mice |
| title_sort | i mastomys natalensis i has a cellular immune response profile distinct from laboratory mice |
| topic | <i>Mastomys natalensis</i> immune response T cell effector cytokines concanavalin A phytohaemagglutinin P |
| url | https://www.mdpi.com/1999-4915/13/5/729 |
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