Acute Toxicity Assay from Seeds and Flesh of Tarap Fruit (Artocarpus odoratissimus Blanco) Ethanolic Extract against Daphnia magna Larvae
Tarap (Artocarpus odoratissimus Blanco) is one of the plants in the tropics that are consumed by dayak tribe in East Kalimantan. Toxicity tests on seeds and bark have been done but there is no data regarding the acute toxicity of Artocarpus odoratissimus Blanco seeds and flesh of fruit causing the n...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mulawarman University
2020-12-01
|
| Series: | Journal of Tropical Pharmacy and Chemistry |
| Subjects: | |
| Online Access: | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/240 |
| _version_ | 1797637433040306176 |
|---|---|
| author | Crissty Magglin Ika Fikriah Khemasili Kosala Hadi Kuncoro |
| author_facet | Crissty Magglin Ika Fikriah Khemasili Kosala Hadi Kuncoro |
| author_sort | Crissty Magglin |
| collection | DOAJ |
| description | Tarap (Artocarpus odoratissimus Blanco) is one of the plants in the tropics that are consumed by dayak tribe in East Kalimantan. Toxicity tests on seeds and bark have been done but there is no data regarding the acute toxicity of Artocarpus odoratissimus Blanco seeds and flesh of fruit causing the need for acute toxicity tests. This Research to know the acute toxic effects of tarap (Artocarpus odoratissimus Blanco) seed and flesh extracts on larvae of Daphnia magna. Tarap seeds and flesh (Artocarpus odoratissimus Blanco) was taken from dayak market in Samarinda, is East Kalimantan, Indonesia. The seeds and flesh of the tarap fruit are extracted by maceration with ethanol solvent. An acute toxicity test was performed by exposing Dapnia magna larvae aged ? 24 hours with a solution of the experimental group and the control group for 48 hours. Toxicity test results are expressed in percentage of immobilization of larvae of Daphnia magna calculated by probit test to obtain EC50 (Half maximal effective concentration) values. Extracts are toxic if the EC50 value > 1000ppm. EC50 Ethanol extract of tarap seeds obtained values ( 3922,301 ± 324,590 ) for EC50 24h and ( 2964,498 ± 412,498 ) for EC50 48h. The EC50 value of ethanol extract from flesh of tarap fruit is ( 12224.514 ± 2186. 899 ) EC50 24h and ( 6165,235 ± 1940,006 ) EC50 48h.Ethanol extract of tarap fruit and flesh is non toxic to larvae of Daphnia magna. |
| first_indexed | 2024-03-11T12:48:20Z |
| format | Article |
| id | doaj.art-0b21bbd6d3b144d1a8dcd90b79799c49 |
| institution | Directory Open Access Journal |
| issn | 2087-7099 2407-6090 |
| language | English |
| last_indexed | 2024-03-11T12:48:20Z |
| publishDate | 2020-12-01 |
| publisher | Mulawarman University |
| record_format | Article |
| series | Journal of Tropical Pharmacy and Chemistry |
| spelling | doaj.art-0b21bbd6d3b144d1a8dcd90b79799c492023-11-04T06:01:05ZengMulawarman UniversityJournal of Tropical Pharmacy and Chemistry2087-70992407-60902020-12-015210.25026/jtpc.v5i2.240Acute Toxicity Assay from Seeds and Flesh of Tarap Fruit (Artocarpus odoratissimus Blanco) Ethanolic Extract against Daphnia magna LarvaeCrissty Magglin0Ika Fikriah1Khemasili Kosala2Hadi Kuncoro3Program Studi Kedokteran, Fakultas Kedokteran, Universitas Mulawarman 2Laboratorium Farmakologi, Fakultas Kedokteran, Universitas MulawarmanLaboratorium Farmakologi, Fakultas Kedokteran, Universitas MulawarmanFakultas Farmasi Universitas Mulawarman , Samarinda, Kalimatan Timur, Indonesia Tarap (Artocarpus odoratissimus Blanco) is one of the plants in the tropics that are consumed by dayak tribe in East Kalimantan. Toxicity tests on seeds and bark have been done but there is no data regarding the acute toxicity of Artocarpus odoratissimus Blanco seeds and flesh of fruit causing the need for acute toxicity tests. This Research to know the acute toxic effects of tarap (Artocarpus odoratissimus Blanco) seed and flesh extracts on larvae of Daphnia magna. Tarap seeds and flesh (Artocarpus odoratissimus Blanco) was taken from dayak market in Samarinda, is East Kalimantan, Indonesia. The seeds and flesh of the tarap fruit are extracted by maceration with ethanol solvent. An acute toxicity test was performed by exposing Dapnia magna larvae aged ? 24 hours with a solution of the experimental group and the control group for 48 hours. Toxicity test results are expressed in percentage of immobilization of larvae of Daphnia magna calculated by probit test to obtain EC50 (Half maximal effective concentration) values. Extracts are toxic if the EC50 value > 1000ppm. EC50 Ethanol extract of tarap seeds obtained values ( 3922,301 ± 324,590 ) for EC50 24h and ( 2964,498 ± 412,498 ) for EC50 48h. The EC50 value of ethanol extract from flesh of tarap fruit is ( 12224.514 ± 2186. 899 ) EC50 24h and ( 6165,235 ± 1940,006 ) EC50 48h.Ethanol extract of tarap fruit and flesh is non toxic to larvae of Daphnia magna.https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/240Artocarpus odoratissimus Blancoacute toxocityDaphnia magna |
| spellingShingle | Crissty Magglin Ika Fikriah Khemasili Kosala Hadi Kuncoro Acute Toxicity Assay from Seeds and Flesh of Tarap Fruit (Artocarpus odoratissimus Blanco) Ethanolic Extract against Daphnia magna Larvae Journal of Tropical Pharmacy and Chemistry Artocarpus odoratissimus Blanco acute toxocity Daphnia magna |
| title | Acute Toxicity Assay from Seeds and Flesh of Tarap Fruit (Artocarpus odoratissimus Blanco) Ethanolic Extract against Daphnia magna Larvae |
| title_full | Acute Toxicity Assay from Seeds and Flesh of Tarap Fruit (Artocarpus odoratissimus Blanco) Ethanolic Extract against Daphnia magna Larvae |
| title_fullStr | Acute Toxicity Assay from Seeds and Flesh of Tarap Fruit (Artocarpus odoratissimus Blanco) Ethanolic Extract against Daphnia magna Larvae |
| title_full_unstemmed | Acute Toxicity Assay from Seeds and Flesh of Tarap Fruit (Artocarpus odoratissimus Blanco) Ethanolic Extract against Daphnia magna Larvae |
| title_short | Acute Toxicity Assay from Seeds and Flesh of Tarap Fruit (Artocarpus odoratissimus Blanco) Ethanolic Extract against Daphnia magna Larvae |
| title_sort | acute toxicity assay from seeds and flesh of tarap fruit artocarpus odoratissimus blanco ethanolic extract against daphnia magna larvae |
| topic | Artocarpus odoratissimus Blanco acute toxocity Daphnia magna |
| url | https://jtpc.farmasi.unmul.ac.id/index.php/jtpc/article/view/240 |
| work_keys_str_mv | AT crisstymagglin acutetoxicityassayfromseedsandfleshoftarapfruitartocarpusodoratissimusblancoethanolicextractagainstdaphniamagnalarvae AT ikafikriah acutetoxicityassayfromseedsandfleshoftarapfruitartocarpusodoratissimusblancoethanolicextractagainstdaphniamagnalarvae AT khemasilikosala acutetoxicityassayfromseedsandfleshoftarapfruitartocarpusodoratissimusblancoethanolicextractagainstdaphniamagnalarvae AT hadikuncoro acutetoxicityassayfromseedsandfleshoftarapfruitartocarpusodoratissimusblancoethanolicextractagainstdaphniamagnalarvae |