Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegans
The causality and mechanism of dietary effects on brain aging are still unclear due to the long time scales of aging. The nematode Caenorhabditis elegans has contributed to aging research because of its short lifespan and easy genetic manipulation. When fed the standard laboratory diet, Escherichia...
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eLife Sciences Publications Ltd
2023-05-01
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| Online Access: | https://elifesciences.org/articles/81418 |
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| author | Satoshi Higurashi Sachio Tsukada Binta Maria Aleogho Joo Hyun Park Yana Al-Hebri Masaru Tanaka Shunji Nakano Ikue Mori Kentaro Noma |
| author_facet | Satoshi Higurashi Sachio Tsukada Binta Maria Aleogho Joo Hyun Park Yana Al-Hebri Masaru Tanaka Shunji Nakano Ikue Mori Kentaro Noma |
| author_sort | Satoshi Higurashi |
| collection | DOAJ |
| description | The causality and mechanism of dietary effects on brain aging are still unclear due to the long time scales of aging. The nematode Caenorhabditis elegans has contributed to aging research because of its short lifespan and easy genetic manipulation. When fed the standard laboratory diet, Escherichia coli, C. elegans experiences an age-dependent decline in temperature–food associative learning, called thermotaxis. To address if diet affects this decline, we screened 35 lactic acid bacteria as alternative diet and found that animals maintained high thermotaxis ability when fed a clade of Lactobacilli enriched with heterofermentative bacteria. Among them, Lactobacillus reuteri maintained the thermotaxis of aged animals without affecting their lifespan and motility. The effect of Lb. reuteri depends on the DAF-16 transcription factor functioning in neurons. Furthermore, RNA sequencing analysis revealed that differentially expressed genes between aged animals fed different bacteria were enriched with DAF-16 targets. Our results demonstrate that diet can impact brain aging in a daf-16-dependent manner without changing the lifespan. |
| first_indexed | 2024-03-13T08:38:41Z |
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| id | doaj.art-0b2590c4a1b1487b91b50e98be64f8fa |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-03-13T08:38:41Z |
| publishDate | 2023-05-01 |
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| spelling | doaj.art-0b2590c4a1b1487b91b50e98be64f8fa2023-05-30T16:19:46ZengeLife Sciences Publications LtdeLife2050-084X2023-05-011210.7554/eLife.81418Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegansSatoshi Higurashi0Sachio Tsukada1Binta Maria Aleogho2Joo Hyun Park3Yana Al-Hebri4Masaru Tanaka5Shunji Nakano6Ikue Mori7Kentaro Noma8https://orcid.org/0000-0002-6487-8037Milk Science Research Institute, Megmilk Snow Brand Co. Ltd., Saitama, Japan; Group of Nutritional Neuroscience, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, JapanMilk Science Research Institute, Megmilk Snow Brand Co. Ltd., Saitama, Japan; Group of Nutritional Neuroscience, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, JapanGroup of Nutritional Neuroscience, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, Japan; Group of Molecular Neurobiology, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, Japan; Group of Microbial Motility, Department of Biological Science, Division of Natural Science, Graduate school of Science, Nagoya University, Nagoya, JapanGroup of Nutritional Neuroscience, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, JapanGroup of Nutritional Neuroscience, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, JapanMilk Science Research Institute, Megmilk Snow Brand Co. Ltd., Saitama, Japan; Group of Nutritional Neuroscience, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, JapanGroup of Molecular Neurobiology, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, JapanGroup of Molecular Neurobiology, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, JapanGroup of Nutritional Neuroscience, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, Japan; Group of Molecular Neurobiology, Neuroscience Institute, Graduate School of Science, Nagoya University, Nagoya, Japan; Group of Microbial Motility, Department of Biological Science, Division of Natural Science, Graduate school of Science, Nagoya University, Nagoya, JapanThe causality and mechanism of dietary effects on brain aging are still unclear due to the long time scales of aging. The nematode Caenorhabditis elegans has contributed to aging research because of its short lifespan and easy genetic manipulation. When fed the standard laboratory diet, Escherichia coli, C. elegans experiences an age-dependent decline in temperature–food associative learning, called thermotaxis. To address if diet affects this decline, we screened 35 lactic acid bacteria as alternative diet and found that animals maintained high thermotaxis ability when fed a clade of Lactobacilli enriched with heterofermentative bacteria. Among them, Lactobacillus reuteri maintained the thermotaxis of aged animals without affecting their lifespan and motility. The effect of Lb. reuteri depends on the DAF-16 transcription factor functioning in neurons. Furthermore, RNA sequencing analysis revealed that differentially expressed genes between aged animals fed different bacteria were enriched with DAF-16 targets. Our results demonstrate that diet can impact brain aging in a daf-16-dependent manner without changing the lifespan.https://elifesciences.org/articles/81418agingbehaviordietlactic acid bacteria |
| spellingShingle | Satoshi Higurashi Sachio Tsukada Binta Maria Aleogho Joo Hyun Park Yana Al-Hebri Masaru Tanaka Shunji Nakano Ikue Mori Kentaro Noma Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegans eLife aging behavior diet lactic acid bacteria |
| title | Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegans |
| title_full | Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegans |
| title_fullStr | Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegans |
| title_full_unstemmed | Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegans |
| title_short | Bacterial diet affects the age-dependent decline of associative learning in Caenorhabditis elegans |
| title_sort | bacterial diet affects the age dependent decline of associative learning in caenorhabditis elegans |
| topic | aging behavior diet lactic acid bacteria |
| url | https://elifesciences.org/articles/81418 |
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