Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field Experiment
Abstract A lack of stability in the expression of Bacillus thuringiensis genes (CRY) and the dialaninophosphate resistance gene (BAR) in transgenic rice plants can lead to the loss of important characters. The genetic stability of transgenic expression in high-generation lines is thus critically imp...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2023-02-01
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| Series: | Rice |
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| Online Access: | https://doi.org/10.1186/s12284-023-00624-5 |
| _version_ | 1797863517039099904 |
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| author | Yue Sun Zhongkai Chen Huizhen Chen Chunlei Wang Bai Li Lu Qin Xiaoli Lin Yicong Cai Dahu Zhou Linjuan Ouyang Changlan Zhu Haohua He Xiaosong Peng |
| author_facet | Yue Sun Zhongkai Chen Huizhen Chen Chunlei Wang Bai Li Lu Qin Xiaoli Lin Yicong Cai Dahu Zhou Linjuan Ouyang Changlan Zhu Haohua He Xiaosong Peng |
| author_sort | Yue Sun |
| collection | DOAJ |
| description | Abstract A lack of stability in the expression of Bacillus thuringiensis genes (CRY) and the dialaninophosphate resistance gene (BAR) in transgenic rice plants can lead to the loss of important characters. The genetic stability of transgenic expression in high-generation lines is thus critically important for ensuring the success of molecular breeding efforts. Here, we studied the genetic stability of resistance to insect pests and herbicides in transgenic rice lines at the molecular and phenotypic levels in a pesticide-free environment. Southern blot analysis, real-time polymerase chain reaction, and enzyme-linked immunosorbent assays revealed high stability in the copy numbers and expression levels of CRY1C, CRY2A, and BAR in transgenic lines across different generations, and gene expression levels were highly correlated with protein expression levels. The insecticide resistance of the transgenic rice lines was high. The larval mortality of Chilo suppressalis was 50.25% to 68.36% higher in transgenic lines than in non-transgenic control lines. Percent dead hearts and percent white spikelets were 16.66% to 22.15% and 27.07% to 33.47% lower in transgenic lines than in non-transgenic control lines, respectively. The herbicide resistance of the transgenic rice lines was also high. The bud length and root length ranged were 2.53 cm to 4.20 cm and 0.28 cm to 0.73 cm higher in transgenic lines than in non-transgenic control lines in the budding stage, respectively. Following application of the herbicide Basta, the chlorophyll content of the transgenic lines began to recover 2 d later in the seedling and tillering stages and 3 d later in the booting and heading stages, by contrast, the chlorophyll content of the non-transgenic lines did not recover and continued to decrease. These findings revealed high genetic stability of the resistance to insect pests and herbicides across several generations of transgenic rice regardless of the genetic background. |
| first_indexed | 2024-04-09T22:36:45Z |
| format | Article |
| id | doaj.art-0b28df87e5e24de899b3e93550b61c81 |
| institution | Directory Open Access Journal |
| issn | 1939-8425 1939-8433 |
| language | English |
| last_indexed | 2024-04-09T22:36:45Z |
| publishDate | 2023-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Rice |
| spelling | doaj.art-0b28df87e5e24de899b3e93550b61c812023-03-22T12:23:18ZengSpringerOpenRice1939-84251939-84332023-02-0116111810.1186/s12284-023-00624-5Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field ExperimentYue Sun0Zhongkai Chen1Huizhen Chen2Chunlei Wang3Bai Li4Lu Qin5Xiaoli Lin6Yicong Cai7Dahu Zhou8Linjuan Ouyang9Changlan Zhu10Haohua He11Xiaosong Peng12Key Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityKey Laboratory of Crop Physiology, Ecology, and Genetic Breeding, Ministry of Education /College of Agronomy, Jiangxi Agricultural UniversityAbstract A lack of stability in the expression of Bacillus thuringiensis genes (CRY) and the dialaninophosphate resistance gene (BAR) in transgenic rice plants can lead to the loss of important characters. The genetic stability of transgenic expression in high-generation lines is thus critically important for ensuring the success of molecular breeding efforts. Here, we studied the genetic stability of resistance to insect pests and herbicides in transgenic rice lines at the molecular and phenotypic levels in a pesticide-free environment. Southern blot analysis, real-time polymerase chain reaction, and enzyme-linked immunosorbent assays revealed high stability in the copy numbers and expression levels of CRY1C, CRY2A, and BAR in transgenic lines across different generations, and gene expression levels were highly correlated with protein expression levels. The insecticide resistance of the transgenic rice lines was high. The larval mortality of Chilo suppressalis was 50.25% to 68.36% higher in transgenic lines than in non-transgenic control lines. Percent dead hearts and percent white spikelets were 16.66% to 22.15% and 27.07% to 33.47% lower in transgenic lines than in non-transgenic control lines, respectively. The herbicide resistance of the transgenic rice lines was also high. The bud length and root length ranged were 2.53 cm to 4.20 cm and 0.28 cm to 0.73 cm higher in transgenic lines than in non-transgenic control lines in the budding stage, respectively. Following application of the herbicide Basta, the chlorophyll content of the transgenic lines began to recover 2 d later in the seedling and tillering stages and 3 d later in the booting and heading stages, by contrast, the chlorophyll content of the non-transgenic lines did not recover and continued to decrease. These findings revealed high genetic stability of the resistance to insect pests and herbicides across several generations of transgenic rice regardless of the genetic background.https://doi.org/10.1186/s12284-023-00624-5Bacillus thuringiensisGenetic stabilityTransgenic riceInsect resistanceHerbicide resistance |
| spellingShingle | Yue Sun Zhongkai Chen Huizhen Chen Chunlei Wang Bai Li Lu Qin Xiaoli Lin Yicong Cai Dahu Zhou Linjuan Ouyang Changlan Zhu Haohua He Xiaosong Peng Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field Experiment Rice Bacillus thuringiensis Genetic stability Transgenic rice Insect resistance Herbicide resistance |
| title | Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field Experiment |
| title_full | Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field Experiment |
| title_fullStr | Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field Experiment |
| title_full_unstemmed | Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field Experiment |
| title_short | Analysis of the Genetic Stability of Insect and Herbicide Resistance Genes in Transgenic Rice Lines: A Laboratory and Field Experiment |
| title_sort | analysis of the genetic stability of insect and herbicide resistance genes in transgenic rice lines a laboratory and field experiment |
| topic | Bacillus thuringiensis Genetic stability Transgenic rice Insect resistance Herbicide resistance |
| url | https://doi.org/10.1186/s12284-023-00624-5 |
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