Cytogenetic study and optimization of genome in situ hybridization (GISH) method in Pistacia spp.
Pistachio (<em>Pistacia vera</em>) is one of the most important horticultural products in the world known by the name of Iran. Moreover, Iran has the richest germplasm of pistachio due to the origin of pistachio and the existence of large numbers of commercial pistachio cultivars. Nevert...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
| Published: |
Shahid Bahonar University of Kerman
2014-11-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_1333_cc9dd9a834ed17380947493b42f5407b.pdf |
| Summary: | Pistachio (<em>Pistacia vera</em>) is one of the most important horticultural products in the world known by the name of Iran. Moreover, Iran has the richest germplasm of pistachio due to the origin of pistachio and the existence of large numbers of commercial pistachio cultivars. Nevertheless, few chromosomal studies in pistachio have been yet reported and they however showed contradicting results on the number of chromosomes. In the present study, different pistachio species including <em>P. vera, </em>and wild species, i.e., <em>Pistacia khinjuk</em> and<em> P. atlantica </em>subsp. <em>m</em><em>utica</em> were studied by classical chromosome staining and GISH technique. In order to stain chromosomes, two methods of aceto-iron-haematoxylin and Feulgen were used. Results indicated that the best staining was achieved by Feulgen compared with another method. Chromosome numbers of ‘Ohadi’, ‘Akbary’, ‘Ahmad-Aghaei’, ‘Nish-Kelaghi’ and ‘Khanjary’ were 2n=30 while chromosome numbers of ‘Italiaei’, ‘Ghazvini’ and ‘Sarakhs’ wild variety were 2n=28. Chromosome numbers of both <em>P. khinjuk </em>and<em> P. atlantica </em>subsp. <em>mutica</em> were 2n=28. In GISH experiment, no signal of hybridization of <em>P. khinjuk</em> and <em>P. atlantica </em>subsp <em>mutica</em> genomic probes were detected on <em>P. vera </em>chromosomes. This lack of detectable hybridization sites may however indicate that these chromosomes are probably originated from different taxa. |
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| ISSN: | 2228-6705 2228-6500 |