Significant Changes in Metabolic Profiles after Intervention with Selenium and Coenzyme Q10 in an Elderly Population
Selenium and coenzyme Q<sub>10</sub> (SeQ<sub>10</sub>) are important for normal cellular function. Low selenium intake leads to increased cardiovascular mortality. Intervention with these substances with healthy elderly persons over a period of four years in a double-blind,...
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MDPI AG
2019-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/9/10/553 |
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| author | Urban Alehagen Peter Johansson Jan Aaseth Jan Alexander Izabella Surowiec Katrin Lundstedt-Enkel Torbjörn Lundstedt |
| author_facet | Urban Alehagen Peter Johansson Jan Aaseth Jan Alexander Izabella Surowiec Katrin Lundstedt-Enkel Torbjörn Lundstedt |
| author_sort | Urban Alehagen |
| collection | DOAJ |
| description | Selenium and coenzyme Q<sub>10</sub> (SeQ<sub>10</sub>) are important for normal cellular function. Low selenium intake leads to increased cardiovascular mortality. Intervention with these substances with healthy elderly persons over a period of four years in a double-blind, randomised placebo-controlled prospective study showed reduced cardiovascular mortality, increased cardiac function, and a lower level of NT-proBNP. Therefore, we wanted to evaluate changes in biochemical pathways as a result of the intervention with SeQ<sub>10</sub> using metabolic profiling. From a population of 443 healthy elderly individuals that were given 200 µg selenium and 200 mg coenzyme Q<sub>10</sub>, or placebo daily for four years, we selected nine males on active intervention and nine males on placebo for metabolic profiling in the main study. To confirm the results, two validation studies (study 1 <i>n</i> = 60 males, study 2 <i>n</i> = 37 males) were conducted. Principal component analyses were used on clinical and demographic data to select representative sets of samples for analysis and to divide the samples into batches for analysis. Gas chromatography time-of-flight mass spectrometry-based metabolomics was applied. The metabolite data were evaluated using univariate and multivariate approaches, mainly T-tests and orthogonal projections to latent structures (OPLS) analyses. Out of 95 identified metabolites, 19 were significantly decreased due to the intervention after 18 months of intervention. Significant changes could be seen in the pentose phosphate, the mevalonate, the beta-oxidation and the xanthine oxidase pathways. The intervention also resulted in changes in the urea cycle, and increases in the levels of the precursors to neurotransmitters of the brain. This adds information to previous published results reporting decreased oxidative stress and inflammation. This is the first-time metabolic profiling has been applied to elucidate the mechanisms behind an intervention with SeQ<sub>10</sub>. The study is small and should be regarded as hypothesis-generating; however, the results are interesting and, therefore, further research in the area is needed. This study was registered at Clinicaltrials.gov, with the identifier NCT01443780. |
| first_indexed | 2024-12-11T13:25:14Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-11T13:25:14Z |
| publishDate | 2019-09-01 |
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| series | Biomolecules |
| spelling | doaj.art-0b29aa370e03470ba56452bf9d8e0c462022-12-22T01:05:34ZengMDPI AGBiomolecules2218-273X2019-09-0191055310.3390/biom9100553biom9100553Significant Changes in Metabolic Profiles after Intervention with Selenium and Coenzyme Q10 in an Elderly PopulationUrban Alehagen0Peter Johansson1Jan Aaseth2Jan Alexander3Izabella Surowiec4Katrin Lundstedt-Enkel5Torbjörn Lundstedt6Division of Cardiovascular Medicine, Department of Medical and Health Sciences, Linköping University, SE-581 85 Linköping, SwedenDepartment of Social and Welfare Studies, Linköping University, SE-581 83 Linköping, SwedenResearch Department, Innlandet Hospital Trust, N-2380 Brumunddal, NorwayNorwegian Institute of Public Health, N-0403 Oslo, NorwayAcureOmics AB, Tvistevägen 48, SE-907 36 Umeå, SwedenAcureOmics AB, Tvistevägen 48, SE-907 36 Umeå, SwedenAcureOmics AB, Tvistevägen 48, SE-907 36 Umeå, SwedenSelenium and coenzyme Q<sub>10</sub> (SeQ<sub>10</sub>) are important for normal cellular function. Low selenium intake leads to increased cardiovascular mortality. Intervention with these substances with healthy elderly persons over a period of four years in a double-blind, randomised placebo-controlled prospective study showed reduced cardiovascular mortality, increased cardiac function, and a lower level of NT-proBNP. Therefore, we wanted to evaluate changes in biochemical pathways as a result of the intervention with SeQ<sub>10</sub> using metabolic profiling. From a population of 443 healthy elderly individuals that were given 200 µg selenium and 200 mg coenzyme Q<sub>10</sub>, or placebo daily for four years, we selected nine males on active intervention and nine males on placebo for metabolic profiling in the main study. To confirm the results, two validation studies (study 1 <i>n</i> = 60 males, study 2 <i>n</i> = 37 males) were conducted. Principal component analyses were used on clinical and demographic data to select representative sets of samples for analysis and to divide the samples into batches for analysis. Gas chromatography time-of-flight mass spectrometry-based metabolomics was applied. The metabolite data were evaluated using univariate and multivariate approaches, mainly T-tests and orthogonal projections to latent structures (OPLS) analyses. Out of 95 identified metabolites, 19 were significantly decreased due to the intervention after 18 months of intervention. Significant changes could be seen in the pentose phosphate, the mevalonate, the beta-oxidation and the xanthine oxidase pathways. The intervention also resulted in changes in the urea cycle, and increases in the levels of the precursors to neurotransmitters of the brain. This adds information to previous published results reporting decreased oxidative stress and inflammation. This is the first-time metabolic profiling has been applied to elucidate the mechanisms behind an intervention with SeQ<sub>10</sub>. The study is small and should be regarded as hypothesis-generating; however, the results are interesting and, therefore, further research in the area is needed. This study was registered at Clinicaltrials.gov, with the identifier NCT01443780.https://www.mdpi.com/2218-273X/9/10/553seleniumcoenzyme q10elderlymetabolic profiling |
| spellingShingle | Urban Alehagen Peter Johansson Jan Aaseth Jan Alexander Izabella Surowiec Katrin Lundstedt-Enkel Torbjörn Lundstedt Significant Changes in Metabolic Profiles after Intervention with Selenium and Coenzyme Q10 in an Elderly Population Biomolecules selenium coenzyme q10 elderly metabolic profiling |
| title | Significant Changes in Metabolic Profiles after Intervention with Selenium and Coenzyme Q10 in an Elderly Population |
| title_full | Significant Changes in Metabolic Profiles after Intervention with Selenium and Coenzyme Q10 in an Elderly Population |
| title_fullStr | Significant Changes in Metabolic Profiles after Intervention with Selenium and Coenzyme Q10 in an Elderly Population |
| title_full_unstemmed | Significant Changes in Metabolic Profiles after Intervention with Selenium and Coenzyme Q10 in an Elderly Population |
| title_short | Significant Changes in Metabolic Profiles after Intervention with Selenium and Coenzyme Q10 in an Elderly Population |
| title_sort | significant changes in metabolic profiles after intervention with selenium and coenzyme q10 in an elderly population |
| topic | selenium coenzyme q10 elderly metabolic profiling |
| url | https://www.mdpi.com/2218-273X/9/10/553 |
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