INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA
An indirect variant of ELISA used for detection of antibodies to nonstructural proteins of the FMD virus in porcine blood sera was developed. The results of the validation showed that the developed method is characterized by high sensitivity, specificity and reproducibility. When testing the blood s...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-10-01
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| Series: | Ветеринария сегодня |
| Subjects: | |
| Online Access: | https://veterinary.arriah.ru/jour/article/view/369 |
| _version_ | 1826812445018030080 |
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| author | A. S. Yakovleva A. V. Kanshina A. V. Scherbakov |
| author_facet | A. S. Yakovleva A. V. Kanshina A. V. Scherbakov |
| author_sort | A. S. Yakovleva |
| collection | DOAJ |
| description | An indirect variant of ELISA used for detection of antibodies to nonstructural proteins of the FMD virus in porcine blood sera was developed. The results of the validation showed that the developed method is characterized by high sensitivity, specificity and reproducibility. When testing the blood serum panel obtained from experimentally infected animals, the method allowed to detect antibodies to FMD virus in 7 of 18 sera collected on day 6 post inoculation, in 13 of 19 sera – on day 7 post inoculation, in 16 of 19 sera – on day 8 post inoculation and in all 76 sera obtained on days 9–12 post inoculation. The diagnostic specificity of 3AB-ELISA was 100% when testing 100 knowingly negative blood sera from pigs imported to Russia from Norway. High specificity and sensitivity of the method, established during the development of the method, are confirmed in the course of routine diagnostic tests. |
| first_indexed | 2024-03-09T02:48:54Z |
| format | Article |
| id | doaj.art-0b2a14b5efe64306a96944406fec6690 |
| institution | Directory Open Access Journal |
| issn | 2304-196X 2658-6959 |
| language | English |
| last_indexed | 2025-02-16T03:58:17Z |
| publishDate | 2018-10-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj.art-0b2a14b5efe64306a96944406fec66902025-02-06T09:52:07ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-10-0103132010.29326/2304-196X-2018-3-26-13-16367INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERAA. S. Yakovleva0A. V. Kanshina1A. V. Scherbakov2FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirAn indirect variant of ELISA used for detection of antibodies to nonstructural proteins of the FMD virus in porcine blood sera was developed. The results of the validation showed that the developed method is characterized by high sensitivity, specificity and reproducibility. When testing the blood serum panel obtained from experimentally infected animals, the method allowed to detect antibodies to FMD virus in 7 of 18 sera collected on day 6 post inoculation, in 13 of 19 sera – on day 7 post inoculation, in 16 of 19 sera – on day 8 post inoculation and in all 76 sera obtained on days 9–12 post inoculation. The diagnostic specificity of 3AB-ELISA was 100% when testing 100 knowingly negative blood sera from pigs imported to Russia from Norway. High specificity and sensitivity of the method, established during the development of the method, are confirmed in the course of routine diagnostic tests.https://veterinary.arriah.ru/jour/article/view/369fmd virusnon-structural proteinselisa test |
| spellingShingle | A. S. Yakovleva A. V. Kanshina A. V. Scherbakov INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA Ветеринария сегодня fmd virus non-structural proteins elisa test |
| title | INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA |
| title_full | INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA |
| title_fullStr | INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA |
| title_full_unstemmed | INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA |
| title_short | INDIRECT ELISA FOR DETECTION OF ANTIBODIES TO FMDV NON-STRUCTURAL PROTEINS IN PORCINE SERA |
| title_sort | indirect elisa for detection of antibodies to fmdv non structural proteins in porcine sera |
| topic | fmd virus non-structural proteins elisa test |
| url | https://veterinary.arriah.ru/jour/article/view/369 |
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