Glutathione imparts stress tolerance against Alternaria brassicicola infection via miRNA mediated gene regulation
Glutathione (GSH) is well known to play a crucial role in imparting resistance against various pathogen invasions. Nevertheless, the role of GSH in regulating miRNA-mediated defense response is yet to be explored. To decipher the GSH-mediated regulation of miRNA expression during necrotrophic infect...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-12-01
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| Series: | Plant Signaling & Behavior |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/15592324.2022.2047352 |
| _version_ | 1797351939336306688 |
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| author | Riddhi Datta Kajal Mandal Priyanka Boro Asma Sultana Sharmila Chattopadhyay |
| author_facet | Riddhi Datta Kajal Mandal Priyanka Boro Asma Sultana Sharmila Chattopadhyay |
| author_sort | Riddhi Datta |
| collection | DOAJ |
| description | Glutathione (GSH) is well known to play a crucial role in imparting resistance against various pathogen invasions. Nevertheless, the role of GSH in regulating miRNA-mediated defense response is yet to be explored. To decipher the GSH-mediated regulation of miRNA expression during necrotrophic infection in Arabidopsis thaliana, wild-type Col-0 and AtECS1, the transgenic line exhibiting enhanced GSH content, were infected with necrotrophic pathogen Alternaria brassicicola. AtECS1 plants exhibited enhanced resistance as compared to wild-type. MiRNA next-generation sequencing (NGS) was performed to compare the miRNA expression in Col-0 and AtECS1 leaves. Under control condition, differentially expressed 96 known miRNAs and 17 novel miRNAs viz. ath-miR8167f, ath-miR1886.3, ath-miR3932b-5p, etc. were identified. However, under infected condition, 73 known and 43 novel differentially expressed miRNAs viz. ath-miR5652, ath-miR160b, ath-miR865-5p, etc. were identified. Functional annotation and enrichment analysis revealed that several miRNAs that target defense-related genes like leucine-rich repeat protein kinase, MYB transcription factors, TCP8, etc. were down regulated in the AtECS1 line, which, in turn, relieves the repression of their target gene expression, leading to resistance against infection. Together, the present investigation suggests that GSH plays a decisive role in modulating the miRNA-mediated regulation of defense-related genes during pathogen invasion. |
| first_indexed | 2024-03-08T13:07:56Z |
| format | Article |
| id | doaj.art-0b2c84b95153499ca468a1069efdb903 |
| institution | Directory Open Access Journal |
| issn | 1559-2316 1559-2324 |
| language | English |
| last_indexed | 2024-03-08T13:07:56Z |
| publishDate | 2022-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Plant Signaling & Behavior |
| spelling | doaj.art-0b2c84b95153499ca468a1069efdb9032024-01-18T15:58:21ZengTaylor & Francis GroupPlant Signaling & Behavior1559-23161559-23242022-12-0117110.1080/15592324.2022.20473522047352Glutathione imparts stress tolerance against Alternaria brassicicola infection via miRNA mediated gene regulationRiddhi Datta0Kajal Mandal1Priyanka Boro2Asma Sultana3Sharmila Chattopadhyay4Dr. A.P.J. Abdul Kalam Government CollegeCSIR-Indian Institute of Chemical BiologyCSIR-Indian Institute of Chemical BiologyJ. K. CollegeCSIR-Indian Institute of Chemical BiologyGlutathione (GSH) is well known to play a crucial role in imparting resistance against various pathogen invasions. Nevertheless, the role of GSH in regulating miRNA-mediated defense response is yet to be explored. To decipher the GSH-mediated regulation of miRNA expression during necrotrophic infection in Arabidopsis thaliana, wild-type Col-0 and AtECS1, the transgenic line exhibiting enhanced GSH content, were infected with necrotrophic pathogen Alternaria brassicicola. AtECS1 plants exhibited enhanced resistance as compared to wild-type. MiRNA next-generation sequencing (NGS) was performed to compare the miRNA expression in Col-0 and AtECS1 leaves. Under control condition, differentially expressed 96 known miRNAs and 17 novel miRNAs viz. ath-miR8167f, ath-miR1886.3, ath-miR3932b-5p, etc. were identified. However, under infected condition, 73 known and 43 novel differentially expressed miRNAs viz. ath-miR5652, ath-miR160b, ath-miR865-5p, etc. were identified. Functional annotation and enrichment analysis revealed that several miRNAs that target defense-related genes like leucine-rich repeat protein kinase, MYB transcription factors, TCP8, etc. were down regulated in the AtECS1 line, which, in turn, relieves the repression of their target gene expression, leading to resistance against infection. Together, the present investigation suggests that GSH plays a decisive role in modulating the miRNA-mediated regulation of defense-related genes during pathogen invasion.http://dx.doi.org/10.1080/15592324.2022.2047352alternaria brassicicolaarabidopsisglutathionemirnaplant defense |
| spellingShingle | Riddhi Datta Kajal Mandal Priyanka Boro Asma Sultana Sharmila Chattopadhyay Glutathione imparts stress tolerance against Alternaria brassicicola infection via miRNA mediated gene regulation Plant Signaling & Behavior alternaria brassicicola arabidopsis glutathione mirna plant defense |
| title | Glutathione imparts stress tolerance against Alternaria brassicicola infection via miRNA mediated gene regulation |
| title_full | Glutathione imparts stress tolerance against Alternaria brassicicola infection via miRNA mediated gene regulation |
| title_fullStr | Glutathione imparts stress tolerance against Alternaria brassicicola infection via miRNA mediated gene regulation |
| title_full_unstemmed | Glutathione imparts stress tolerance against Alternaria brassicicola infection via miRNA mediated gene regulation |
| title_short | Glutathione imparts stress tolerance against Alternaria brassicicola infection via miRNA mediated gene regulation |
| title_sort | glutathione imparts stress tolerance against alternaria brassicicola infection via mirna mediated gene regulation |
| topic | alternaria brassicicola arabidopsis glutathione mirna plant defense |
| url | http://dx.doi.org/10.1080/15592324.2022.2047352 |
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