Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle
Abstract Background Alopecia areata (AA) is a common autoimmune hair loss disease with increasing incidence. Corticosteroids are the most widely used for hair loss treatment; however, long-term usage of hormonal drugs is associated with various side effects. Mesenchymal stem cells (MSCs) therapy has...
| Main Authors: | , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2021-10-01
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| Series: | Stem Cell Research & Therapy |
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| Online Access: | https://doi.org/10.1186/s13287-021-02614-0 |
| _version_ | 1818929759590023168 |
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| author | Weiyue Deng Yuying Zhang Wei Wang Aishi Song Omar Mukama Jiarong Huang Xiaobo Han Sihao Deng Zuoxian Lin Jean du Dieu Habimana Rongqi Huang Kexin Peng Bing Ni Shusheng Zhang Xiaoxin Yan Ji Li Lin-Ping Wu Zhiyuan Li |
| author_facet | Weiyue Deng Yuying Zhang Wei Wang Aishi Song Omar Mukama Jiarong Huang Xiaobo Han Sihao Deng Zuoxian Lin Jean du Dieu Habimana Rongqi Huang Kexin Peng Bing Ni Shusheng Zhang Xiaoxin Yan Ji Li Lin-Ping Wu Zhiyuan Li |
| author_sort | Weiyue Deng |
| collection | DOAJ |
| description | Abstract Background Alopecia areata (AA) is a common autoimmune hair loss disease with increasing incidence. Corticosteroids are the most widely used for hair loss treatment; however, long-term usage of hormonal drugs is associated with various side effects. Mesenchymal stem cells (MSCs) therapy has been studied extensively to curb autoimmune diseases without affecting immunity against diseases. Methods Hair follicle-derived MSCs (HF-MSCs) were harvested from the waste material of hair transplants, isolated and expanded. The therapeutic effect of HF-MSCs for AA treatment was investigated in vitro AA-like hair follicle organ model and in vivo C3H/HeJ AA mice model. Results AA-like hair follicle organ in vitro model was successfully established by pre-treatment of mouse vibrissa follicles by interferon-γ (IFN-γ). The AA-like symptoms were relieved when IFN-γ induced AA in vitro model was co-cultured with HF-MSC for 2 days. In addition, when skin grafted C3H/HeJ AA mice models were injected with 106 HF-MSCs once a week for 3 weeks, the transcription profiling and immunofluorescence analysis depicted that HF-MSCs treatment significantly decreased mouse hair loss and reduced inflammation around HF both in vitro and in vivo. Conclusions This study provides a new therapeutic approach for alopecia areata based on HF-MSCs toward its future clinical application. |
| first_indexed | 2024-12-20T03:49:55Z |
| format | Article |
| id | doaj.art-0b38c8d9e8de4ad88658cf85e346803a |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-20T03:49:55Z |
| publishDate | 2021-10-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-0b38c8d9e8de4ad88658cf85e346803a2022-12-21T19:54:29ZengBMCStem Cell Research & Therapy1757-65122021-10-0112111110.1186/s13287-021-02614-0Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicleWeiyue Deng0Yuying Zhang1Wei Wang2Aishi Song3Omar Mukama4Jiarong Huang5Xiaobo Han6Sihao Deng7Zuoxian Lin8Jean du Dieu Habimana9Rongqi Huang10Kexin Peng11Bing Ni12Shusheng Zhang13Xiaoxin Yan14Ji Li15Lin-Ping Wu16Zhiyuan Li17Department of Anatomy and Neurobiology, Xiangya School of Medicine, Central South UniversityDepartment of Anatomy and Neurobiology, Xiangya School of Medicine, Central South UniversityDepartment of Anatomy and Neurobiology, Xiangya School of Medicine, Central South UniversityDepartment of Anatomy and Neurobiology, Xiangya School of Medicine, Central South UniversityCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCenter for Chemical Biology and Drug Discovery, Guangzhou Institute of Biomedicine and Health, Chinese Academy of SciencesCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesDepartment of Anatomy and Neurobiology, Xiangya School of Medicine, Central South UniversityCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesCAS Key Laboratory of Regenerative Biology, Guangdong Provincial Key Laboratory of Stem Cell and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Chinese Academy of SciencesNHC Key Laboratory of Birth Defect for Research and Prevention, Hunan Provincial Maternal and Child Health Care HospitalNHC Key Laboratory of Birth Defect for Research and Prevention, Hunan Provincial Maternal and Child Health Care HospitalChangsha Stomatological HospitalDepartment of Anatomy and Neurobiology, Xiangya School of Medicine, Central South UniversityDepartment of Anatomy and Neurobiology, Xiangya School of Medicine, Central South UniversityCenter for Chemical Biology and Drug Discovery, Guangzhou Institute of Biomedicine and Health, Chinese Academy of SciencesDepartment of Anatomy and Neurobiology, Xiangya School of Medicine, Central South UniversityAbstract Background Alopecia areata (AA) is a common autoimmune hair loss disease with increasing incidence. Corticosteroids are the most widely used for hair loss treatment; however, long-term usage of hormonal drugs is associated with various side effects. Mesenchymal stem cells (MSCs) therapy has been studied extensively to curb autoimmune diseases without affecting immunity against diseases. Methods Hair follicle-derived MSCs (HF-MSCs) were harvested from the waste material of hair transplants, isolated and expanded. The therapeutic effect of HF-MSCs for AA treatment was investigated in vitro AA-like hair follicle organ model and in vivo C3H/HeJ AA mice model. Results AA-like hair follicle organ in vitro model was successfully established by pre-treatment of mouse vibrissa follicles by interferon-γ (IFN-γ). The AA-like symptoms were relieved when IFN-γ induced AA in vitro model was co-cultured with HF-MSC for 2 days. In addition, when skin grafted C3H/HeJ AA mice models were injected with 106 HF-MSCs once a week for 3 weeks, the transcription profiling and immunofluorescence analysis depicted that HF-MSCs treatment significantly decreased mouse hair loss and reduced inflammation around HF both in vitro and in vivo. Conclusions This study provides a new therapeutic approach for alopecia areata based on HF-MSCs toward its future clinical application.https://doi.org/10.1186/s13287-021-02614-0Alopecia areataHair follicle-derived mesenchymal stem cellsStem cell therapyHair loss treatment |
| spellingShingle | Weiyue Deng Yuying Zhang Wei Wang Aishi Song Omar Mukama Jiarong Huang Xiaobo Han Sihao Deng Zuoxian Lin Jean du Dieu Habimana Rongqi Huang Kexin Peng Bing Ni Shusheng Zhang Xiaoxin Yan Ji Li Lin-Ping Wu Zhiyuan Li Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle Stem Cell Research & Therapy Alopecia areata Hair follicle-derived mesenchymal stem cells Stem cell therapy Hair loss treatment |
| title | Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle |
| title_full | Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle |
| title_fullStr | Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle |
| title_full_unstemmed | Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle |
| title_short | Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle |
| title_sort | hair follicle derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle |
| topic | Alopecia areata Hair follicle-derived mesenchymal stem cells Stem cell therapy Hair loss treatment |
| url | https://doi.org/10.1186/s13287-021-02614-0 |
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