UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes.
JIP3/UNC-16/dSYD is a MAPK-scaffolding protein with roles in protein trafficking. We show that it is present on the Golgi and is necessary for the polarized distribution of synaptic vesicle proteins (SVPs) and dendritic proteins in neurons. UNC-16 excludes Golgi enzymes from SVP transport carriers a...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-11-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC5716593?pdf=render |
| _version_ | 1819104717600456704 |
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| author | Bikash Choudhary Madhushree Kamak Neena Ratnakaran Jitendra Kumar Anjali Awasthi Chun Li Ken Nguyen Kunihiro Matsumoto Naoki Hisamoto Sandhya P Koushika |
| author_facet | Bikash Choudhary Madhushree Kamak Neena Ratnakaran Jitendra Kumar Anjali Awasthi Chun Li Ken Nguyen Kunihiro Matsumoto Naoki Hisamoto Sandhya P Koushika |
| author_sort | Bikash Choudhary |
| collection | DOAJ |
| description | JIP3/UNC-16/dSYD is a MAPK-scaffolding protein with roles in protein trafficking. We show that it is present on the Golgi and is necessary for the polarized distribution of synaptic vesicle proteins (SVPs) and dendritic proteins in neurons. UNC-16 excludes Golgi enzymes from SVP transport carriers and facilitates inclusion of specific SVPs into the same transport carrier. The SVP trafficking roles of UNC-16 are mediated through LRK-1, whose localization to the Golgi is reduced in unc-16 animals. UNC-16, through LRK-1, also enables Golgi-localization of the μ-subunit of the AP-1 complex. AP1 regulates the size but not the composition of SVP transport carriers. Additionally, UNC-16 and LRK-1 through the AP-3 complex regulates the composition but not the size of the SVP transport carrier. These early biogenesis steps are essential for dependence on the synaptic vesicle motor, UNC-104 for axonal transport. Our results show that UNC-16 and its downstream effectors, LRK-1 and the AP complexes function at the Golgi and/or post-Golgi compartments to control early steps of SV biogenesis. The UNC-16 dependent steps of exclusion, inclusion and motor recruitment are critical for polarized distribution of neuronal cargo. |
| first_indexed | 2024-12-22T02:10:48Z |
| format | Article |
| id | doaj.art-0b3b36cf1b74415c83b6a5fecb1c3074 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-22T02:10:48Z |
| publishDate | 2017-11-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-0b3b36cf1b74415c83b6a5fecb1c30742022-12-21T18:42:24ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042017-11-011311e100710010.1371/journal.pgen.1007100UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes.Bikash ChoudharyMadhushree KamakNeena RatnakaranJitendra KumarAnjali AwasthiChun LiKen NguyenKunihiro MatsumotoNaoki HisamotoSandhya P KoushikaJIP3/UNC-16/dSYD is a MAPK-scaffolding protein with roles in protein trafficking. We show that it is present on the Golgi and is necessary for the polarized distribution of synaptic vesicle proteins (SVPs) and dendritic proteins in neurons. UNC-16 excludes Golgi enzymes from SVP transport carriers and facilitates inclusion of specific SVPs into the same transport carrier. The SVP trafficking roles of UNC-16 are mediated through LRK-1, whose localization to the Golgi is reduced in unc-16 animals. UNC-16, through LRK-1, also enables Golgi-localization of the μ-subunit of the AP-1 complex. AP1 regulates the size but not the composition of SVP transport carriers. Additionally, UNC-16 and LRK-1 through the AP-3 complex regulates the composition but not the size of the SVP transport carrier. These early biogenesis steps are essential for dependence on the synaptic vesicle motor, UNC-104 for axonal transport. Our results show that UNC-16 and its downstream effectors, LRK-1 and the AP complexes function at the Golgi and/or post-Golgi compartments to control early steps of SV biogenesis. The UNC-16 dependent steps of exclusion, inclusion and motor recruitment are critical for polarized distribution of neuronal cargo.http://europepmc.org/articles/PMC5716593?pdf=render |
| spellingShingle | Bikash Choudhary Madhushree Kamak Neena Ratnakaran Jitendra Kumar Anjali Awasthi Chun Li Ken Nguyen Kunihiro Matsumoto Naoki Hisamoto Sandhya P Koushika UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes. PLoS Genetics |
| title | UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes. |
| title_full | UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes. |
| title_fullStr | UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes. |
| title_full_unstemmed | UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes. |
| title_short | UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes. |
| title_sort | unc 16 jip3 regulates early events in synaptic vesicle protein trafficking via lrk 1 lrrk2 and ap complexes |
| url | http://europepmc.org/articles/PMC5716593?pdf=render |
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