In Vivo Assessment of Anti-Tumor Activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on 7,12 dimethylbenz(a) Anthracene-Induced Mammary Tumors in Rat
Objective- Breast cancer is one of the most serious problems in oncology. Alkaloids pyrrolizidine are widely found in nature and exhibited versatile biological activities. The aim of the present study was to assess anti-tumor activity of methyl 1,3-dioxo-1,1',2',3,5',6',7',7...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iranian Veterinary Surgery Association (IVSA)
2017-12-01
|
| Series: | Iranian Journal of Veterinary Surgery |
| Subjects: | |
| Online Access: | http://www.ivsajournals.com/article_57950_77617eb06832defa2a33ae58af9c716e.pdf |
| _version_ | 1818985371766095872 |
|---|---|
| author | Leila Zarei Mehri Kouhkan Ali Bolouri Rahim Mohammadi |
| author_facet | Leila Zarei Mehri Kouhkan Ali Bolouri Rahim Mohammadi |
| author_sort | Leila Zarei |
| collection | DOAJ |
| description | Objective- Breast cancer is one of the most serious problems in oncology. Alkaloids pyrrolizidine are widely found in nature and exhibited versatile biological activities. The aim of the present study was to assess anti-tumor activity of methyl 1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2'-carboxylate (6) on 7,12 dimethylbenz(a) anthracene (DMBA)-induced mammary tumors in rat. <br /> Design- Experimental study.<br /> Animals- Twenty-one female Wistar ats<br /> Procedures- 7-Week-old female Wistar rats (180 g body weight) were randomized into three groups of seven animals each. DMBA-induced mammary tumors was achived using DMBA dissolved in 1ml of vehicle (0.5 ml of DMSO plus 0.5 ml of saline) and injected by subcutaneous injection beneath the mammary gland on either side. Tumor yield and size were stabilized after 90 days with the initiation of DMBA. Healthy intact animals (NC) were considered as a normal control feeding on pellets and tap water. In group DMBA, the tumor was induced by DMBA. In group DMBA/7d the animals with tumor received 100 µL 6 dissolved in DMSO (0.25 µM) solution intraperitoneally for one week. Synthesis of 6 was accomplished and the structure of new product was assigned by their 1H/13C NMR, IR, CH-COSY and mass spectral data as well as the elemental analysis. <br /> Results- The overall tumor analysis showed that 6 treatment significantly inhibited the breast tumor incidence, tumor multiplicity, and tumor size in the DMBA-initiated rat model. 6 treatment significantly inhibited the total volume of tumors per rat. <br /> Conclusion and Clinical Relevance-The findings of the present study showed that the 6 treatment was significantly effective in reduction of tumors versus the cancer controls. |
| first_indexed | 2024-12-20T18:33:50Z |
| format | Article |
| id | doaj.art-0b3dcc7b96074bb09ac1a2597ceaf5ad |
| institution | Directory Open Access Journal |
| issn | 2008-3033 2008-3033 |
| language | English |
| last_indexed | 2024-12-20T18:33:50Z |
| publishDate | 2017-12-01 |
| publisher | Iranian Veterinary Surgery Association (IVSA) |
| record_format | Article |
| series | Iranian Journal of Veterinary Surgery |
| spelling | doaj.art-0b3dcc7b96074bb09ac1a2597ceaf5ad2022-12-21T19:29:58ZengIranian Veterinary Surgery Association (IVSA)Iranian Journal of Veterinary Surgery2008-30332008-30332017-12-01122536210.22034/ivsa.2018.111321.113157950In Vivo Assessment of Anti-Tumor Activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on 7,12 dimethylbenz(a) Anthracene-Induced Mammary Tumors in RatLeila Zarei0Mehri Kouhkan1Ali Bolouri2Rahim Mohammadi3Razi Herbal Medicines Research Center, Lorestan University of medical Sciences, Khorramabad, Iran.Department of Medical Chemistry, School of Pharmacy, Urmia University of Medical Sciences, Urmia, IranDepartment of Medical Chemistry, School of Pharmacy, Urmia University of Medical Sciences, Urmia, IranDepartment of Surgery and Diagnostic Imaging, Faculty of Veterinary Medicine, Urmia UniversityObjective- Breast cancer is one of the most serious problems in oncology. Alkaloids pyrrolizidine are widely found in nature and exhibited versatile biological activities. The aim of the present study was to assess anti-tumor activity of methyl 1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2'-carboxylate (6) on 7,12 dimethylbenz(a) anthracene (DMBA)-induced mammary tumors in rat. <br /> Design- Experimental study.<br /> Animals- Twenty-one female Wistar ats<br /> Procedures- 7-Week-old female Wistar rats (180 g body weight) were randomized into three groups of seven animals each. DMBA-induced mammary tumors was achived using DMBA dissolved in 1ml of vehicle (0.5 ml of DMSO plus 0.5 ml of saline) and injected by subcutaneous injection beneath the mammary gland on either side. Tumor yield and size were stabilized after 90 days with the initiation of DMBA. Healthy intact animals (NC) were considered as a normal control feeding on pellets and tap water. In group DMBA, the tumor was induced by DMBA. In group DMBA/7d the animals with tumor received 100 µL 6 dissolved in DMSO (0.25 µM) solution intraperitoneally for one week. Synthesis of 6 was accomplished and the structure of new product was assigned by their 1H/13C NMR, IR, CH-COSY and mass spectral data as well as the elemental analysis. <br /> Results- The overall tumor analysis showed that 6 treatment significantly inhibited the breast tumor incidence, tumor multiplicity, and tumor size in the DMBA-initiated rat model. 6 treatment significantly inhibited the total volume of tumors per rat. <br /> Conclusion and Clinical Relevance-The findings of the present study showed that the 6 treatment was significantly effective in reduction of tumors versus the cancer controls.http://www.ivsajournals.com/article_57950_77617eb06832defa2a33ae58af9c716e.pdfBreast carcinogenesis7-12-Dimethylbenz(a)anthraceneIndene-2-3'-pyrrolizidinemammary tumors |
| spellingShingle | Leila Zarei Mehri Kouhkan Ali Bolouri Rahim Mohammadi In Vivo Assessment of Anti-Tumor Activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on 7,12 dimethylbenz(a) Anthracene-Induced Mammary Tumors in Rat Iranian Journal of Veterinary Surgery Breast carcinogenesis 7-12-Dimethylbenz(a)anthracene Indene-2-3'-pyrrolizidine mammary tumors |
| title | In Vivo Assessment of Anti-Tumor Activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on 7,12 dimethylbenz(a) Anthracene-Induced Mammary Tumors in Rat |
| title_full | In Vivo Assessment of Anti-Tumor Activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on 7,12 dimethylbenz(a) Anthracene-Induced Mammary Tumors in Rat |
| title_fullStr | In Vivo Assessment of Anti-Tumor Activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on 7,12 dimethylbenz(a) Anthracene-Induced Mammary Tumors in Rat |
| title_full_unstemmed | In Vivo Assessment of Anti-Tumor Activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on 7,12 dimethylbenz(a) Anthracene-Induced Mammary Tumors in Rat |
| title_short | In Vivo Assessment of Anti-Tumor Activity of Methyl 2'-Methyl-1,3-dioxo-1,1',2',3,5',6',7',7a'-octahydrospiro[indene-2,3'-pyrrolizidine]-2' carboxylate (6) on 7,12 dimethylbenz(a) Anthracene-Induced Mammary Tumors in Rat |
| title_sort | in vivo assessment of anti tumor activity of methyl 2 methyl 1 3 dioxo 1 1 2 3 5 6 7 7a octahydrospiro indene 2 3 pyrrolizidine 2 carboxylate 6 on 7 12 dimethylbenz a anthracene induced mammary tumors in rat |
| topic | Breast carcinogenesis 7-12-Dimethylbenz(a)anthracene Indene-2-3'-pyrrolizidine mammary tumors |
| url | http://www.ivsajournals.com/article_57950_77617eb06832defa2a33ae58af9c716e.pdf |
| work_keys_str_mv | AT leilazarei invivoassessmentofantitumoractivityofmethyl2methyl13dioxo11235677aoctahydrospiroindene23pyrrolizidine2carboxylate6on712dimethylbenzaanthraceneinducedmammarytumorsinrat AT mehrikouhkan invivoassessmentofantitumoractivityofmethyl2methyl13dioxo11235677aoctahydrospiroindene23pyrrolizidine2carboxylate6on712dimethylbenzaanthraceneinducedmammarytumorsinrat AT alibolouri invivoassessmentofantitumoractivityofmethyl2methyl13dioxo11235677aoctahydrospiroindene23pyrrolizidine2carboxylate6on712dimethylbenzaanthraceneinducedmammarytumorsinrat AT rahimmohammadi invivoassessmentofantitumoractivityofmethyl2methyl13dioxo11235677aoctahydrospiroindene23pyrrolizidine2carboxylate6on712dimethylbenzaanthraceneinducedmammarytumorsinrat |