Effect of Sonication on Microwave Inactivation Kinetics of <i>Enterococcus faecalis</i> in Dairy Effluent
The aim of this study is to inactivate <i>Enterococcus faecalis</i> ATCC 29212 present in dairy wastewater effluent using microwave (MW) waves and/or ultrasound waves (US). The ultrasonic bath treatment (35 kHz) had no significant effect on the reduction of the survival rate (predominant...
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MDPI AG
2022-11-01
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| Online Access: | https://www.mdpi.com/1420-3049/27/21/7422 |
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| author | Ourdia-Nouara Kernou Amine Belbahi Yasmine Sahraoui Kenza Bedjaoui Kamelia Kerdouche Akila Amir Farid Dahmoune Khodir Madani Patricia Rijo |
| author_facet | Ourdia-Nouara Kernou Amine Belbahi Yasmine Sahraoui Kenza Bedjaoui Kamelia Kerdouche Akila Amir Farid Dahmoune Khodir Madani Patricia Rijo |
| author_sort | Ourdia-Nouara Kernou |
| collection | DOAJ |
| description | The aim of this study is to inactivate <i>Enterococcus faecalis</i> ATCC 29212 present in dairy wastewater effluent using microwave (MW) waves and/or ultrasound waves (US). The ultrasonic bath treatment (35 kHz) had no significant effect on the reduction of the survival rate (predominant declumping effect). At 650 W of microwave treatment, the total destruction was completed at 75 s, while at 350 W a 3 log reduction was achieved. The Weibull model was fitted to the survival curves to describe the inactivation kinetics, and the effect of the combined microwave-ultrasound treatments was evaluated. The scaling parameter <i>α</i> that was estimated from the inactivation kinetics for the microwaves combined with the ultrasound waves in pre-treatment was found to be lower than the scaling parameters obtained in post-treatment, which were in turn lower than those estimated for microwaves or ultrasound waves alone. The use of the ultrasound waves in pre-treatment was more effective than in post-treatment; a total reduction was achieved using a combination of US (30 min) followed by MW (650 W) with <i>α</i> = 28.3 s, while 4.0 log was obtained by reversing all processes with <i>α</i> = 34.5 s. The results from the protein assays indicate that the bacterial wall was damaged and that holes were formed from which protein leakage occurred. |
| first_indexed | 2024-03-09T18:47:37Z |
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| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-09T18:47:37Z |
| publishDate | 2022-11-01 |
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| series | Molecules |
| spelling | doaj.art-0b43bde819614babbdad592893b9104e2023-11-24T06:03:58ZengMDPI AGMolecules1420-30492022-11-012721742210.3390/molecules27217422Effect of Sonication on Microwave Inactivation Kinetics of <i>Enterococcus faecalis</i> in Dairy EffluentOurdia-Nouara Kernou0Amine Belbahi1Yasmine Sahraoui2Kenza Bedjaoui3Kamelia Kerdouche4Akila Amir5Farid Dahmoune6Khodir Madani7Patricia Rijo8Laboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, AlgeriaDepartment of Microbiology and Biochemistry, Faculty of Sciences, University of M’Sila, M’Sila 24000, AlgeriaDepartment of Biology, University M’Hamed Bougara of Boumerdès, Boumerdès 35000, AlgeriaLaboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, AlgeriaLaboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, AlgeriaLaboratoire de Biomathématiques, Biophysique, Biochimie, et Scientométrie (L3BS), Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, Bejaia 06000, AlgeriaDepartement de Biologie, Faculté des Sciences de la Nature et de La Vie et des Sciences de La Terre, Université de Bouira, Bouira 1000, AlgeriaCentre de Recherche en Technologie Agroalimentaire, Route de Targua-Ouzemour, Bejaia 06000, AlgeriaCBIOS-Centro de Investigação em Biociências e Tecnologias da Saúde, Universidade Lusófona, Campo Grande 376, 1749-028 Lisbon, PortugalThe aim of this study is to inactivate <i>Enterococcus faecalis</i> ATCC 29212 present in dairy wastewater effluent using microwave (MW) waves and/or ultrasound waves (US). The ultrasonic bath treatment (35 kHz) had no significant effect on the reduction of the survival rate (predominant declumping effect). At 650 W of microwave treatment, the total destruction was completed at 75 s, while at 350 W a 3 log reduction was achieved. The Weibull model was fitted to the survival curves to describe the inactivation kinetics, and the effect of the combined microwave-ultrasound treatments was evaluated. The scaling parameter <i>α</i> that was estimated from the inactivation kinetics for the microwaves combined with the ultrasound waves in pre-treatment was found to be lower than the scaling parameters obtained in post-treatment, which were in turn lower than those estimated for microwaves or ultrasound waves alone. The use of the ultrasound waves in pre-treatment was more effective than in post-treatment; a total reduction was achieved using a combination of US (30 min) followed by MW (650 W) with <i>α</i> = 28.3 s, while 4.0 log was obtained by reversing all processes with <i>α</i> = 34.5 s. The results from the protein assays indicate that the bacterial wall was damaged and that holes were formed from which protein leakage occurred.https://www.mdpi.com/1420-3049/27/21/7422dairy wastewater effluent<i>Enterococcus faecalis</i>ultrasoundmicrowaveinactivation kinetic modeling |
| spellingShingle | Ourdia-Nouara Kernou Amine Belbahi Yasmine Sahraoui Kenza Bedjaoui Kamelia Kerdouche Akila Amir Farid Dahmoune Khodir Madani Patricia Rijo Effect of Sonication on Microwave Inactivation Kinetics of <i>Enterococcus faecalis</i> in Dairy Effluent Molecules dairy wastewater effluent <i>Enterococcus faecalis</i> ultrasound microwave inactivation kinetic modeling |
| title | Effect of Sonication on Microwave Inactivation Kinetics of <i>Enterococcus faecalis</i> in Dairy Effluent |
| title_full | Effect of Sonication on Microwave Inactivation Kinetics of <i>Enterococcus faecalis</i> in Dairy Effluent |
| title_fullStr | Effect of Sonication on Microwave Inactivation Kinetics of <i>Enterococcus faecalis</i> in Dairy Effluent |
| title_full_unstemmed | Effect of Sonication on Microwave Inactivation Kinetics of <i>Enterococcus faecalis</i> in Dairy Effluent |
| title_short | Effect of Sonication on Microwave Inactivation Kinetics of <i>Enterococcus faecalis</i> in Dairy Effluent |
| title_sort | effect of sonication on microwave inactivation kinetics of i enterococcus faecalis i in dairy effluent |
| topic | dairy wastewater effluent <i>Enterococcus faecalis</i> ultrasound microwave inactivation kinetic modeling |
| url | https://www.mdpi.com/1420-3049/27/21/7422 |
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