Experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of <em>Methanothermobacter marburgensis</em> in a biological methane production process (BMPP)
Recently, interests for new biofuel generations allowing conversion of gaseous substrate(s) to gaseous product(s) arose for power to gas and waste to value applications. An example is biological methane production process (BMPP) with <em>Methanothermobacter marburgensis.</em> The latter,...
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| Format: | Article |
| Language: | English |
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AIMS Press
2016-08-01
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| Series: | AIMS Microbiology |
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| Online Access: | http://www.aimspress.com/microbiology/article/900/fulltext.html |
| _version_ | 1818240623945187328 |
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| author | Alexander Krajete Christoph Herwig Sébastien Bernacchi |
| author_facet | Alexander Krajete Christoph Herwig Sébastien Bernacchi |
| author_sort | Alexander Krajete |
| collection | DOAJ |
| description | Recently, interests for new biofuel generations allowing conversion of gaseous substrate(s) to gaseous product(s) arose for power to gas and waste to value applications. An example is biological methane production process (BMPP) with <em>Methanothermobacter marburgensis.</em> The latter, can convert carbon dioxide (CO<sub>2</sub>) and hydrogen (H<sub>2</sub>), having different origins and purities, to methane (CH<sub>4</sub>), water and biomass. However, these gas converting bioprocesses are tendentiously gas limited processes and the specific methane productivity per biomass amount (qCH<sub>4</sub>) tends to be low. Therefore, this contribution proposes a workflow for the development of a feed forward strategy to control biomass, growth (r<sub>x</sub>) and q<sub>CH4</sub> in a continuous gas limited BMPP. The proposed workflow starts with a design of experiment (DoE) to optimize media composition and search for a liquid based limitation to control selectively growth. From the DoE it came out that controlling biomass growth was possible independently of the dilution and gassing rate applied while not affecting methane evolution rates (MERs). This was done by shifting the process from a natural gas limited state to a controlled liquid limited growth. The latter allowed exploiting the maximum biocatalytic activity for methane formation of <em>Methanothermobacter marburgensis</em>. An increase of q<sub>CH4</sub> from 42 to 129 mmol<sub>CH4</sub> g<sup>−1</sup> h<sup>−1</sup> was achieved by applying a liquid limitation compare with the reference state. Finally, a verification experiment was done to verify the feeding strategy transferability to a different process configuration. This evidenced the ratio of the fed KH<sub>2</sub>PO<sub>4</sub> to r<sub>x</sub> (R(F<sub>KH2PO4</sub>/r<sub>x</sub>)) has an appropriate parameter for scaling feeds in a continuous gas limited BMPP. In the verification experiment CH<sub>4</sub> was produced in a single bioreactor step at a methane evolution rate (MER) of 132 mmol<sub>CH4</sub>*L<sup>−1</sup>*h<sup>−1</sup> at a CH<sub>4</sub> purity of 93 [Vol.%]. |
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| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2471-1888 |
| language | English |
| last_indexed | 2024-12-12T13:16:24Z |
| publishDate | 2016-08-01 |
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| spelling | doaj.art-0b452c1112b041aab2e7225d103619ca2022-12-22T00:23:24ZengAIMS PressAIMS Microbiology2471-18882016-08-012326227710.3934/microbiol.2016.3.262microbiol-02-00262Experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of <em>Methanothermobacter marburgensis</em> in a biological methane production process (BMPP)Alexander Krajete0Christoph Herwig1Sébastien BernacchiKrajete GmbH, Scharitzerstraße 30, 4020 Linz, AustriaInstitute of Chemical Engineering, Vienna University of Technology, Gumpendorferstraße 1a, 1060 Vienna, AustriaRecently, interests for new biofuel generations allowing conversion of gaseous substrate(s) to gaseous product(s) arose for power to gas and waste to value applications. An example is biological methane production process (BMPP) with <em>Methanothermobacter marburgensis.</em> The latter, can convert carbon dioxide (CO<sub>2</sub>) and hydrogen (H<sub>2</sub>), having different origins and purities, to methane (CH<sub>4</sub>), water and biomass. However, these gas converting bioprocesses are tendentiously gas limited processes and the specific methane productivity per biomass amount (qCH<sub>4</sub>) tends to be low. Therefore, this contribution proposes a workflow for the development of a feed forward strategy to control biomass, growth (r<sub>x</sub>) and q<sub>CH4</sub> in a continuous gas limited BMPP. The proposed workflow starts with a design of experiment (DoE) to optimize media composition and search for a liquid based limitation to control selectively growth. From the DoE it came out that controlling biomass growth was possible independently of the dilution and gassing rate applied while not affecting methane evolution rates (MERs). This was done by shifting the process from a natural gas limited state to a controlled liquid limited growth. The latter allowed exploiting the maximum biocatalytic activity for methane formation of <em>Methanothermobacter marburgensis</em>. An increase of q<sub>CH4</sub> from 42 to 129 mmol<sub>CH4</sub> g<sup>−1</sup> h<sup>−1</sup> was achieved by applying a liquid limitation compare with the reference state. Finally, a verification experiment was done to verify the feeding strategy transferability to a different process configuration. This evidenced the ratio of the fed KH<sub>2</sub>PO<sub>4</sub> to r<sub>x</sub> (R(F<sub>KH2PO4</sub>/r<sub>x</sub>)) has an appropriate parameter for scaling feeds in a continuous gas limited BMPP. In the verification experiment CH<sub>4</sub> was produced in a single bioreactor step at a methane evolution rate (MER) of 132 mmol<sub>CH4</sub>*L<sup>−1</sup>*h<sup>−1</sup> at a CH<sub>4</sub> purity of 93 [Vol.%].http://www.aimspress.com/microbiology/article/900/fulltext.htmldesign of experimentschemostatbioprocess quantificationcarbon balancebiological methanogenesisgas limited bioprocesscontinuous bioprocesspower to gasliquid limited growthwaste to value |
| spellingShingle | Alexander Krajete Christoph Herwig Sébastien Bernacchi Experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of <em>Methanothermobacter marburgensis</em> in a biological methane production process (BMPP) AIMS Microbiology design of experiments chemostat bioprocess quantification carbon balance biological methanogenesis gas limited bioprocess continuous bioprocess power to gas liquid limited growth waste to value |
| title | Experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of <em>Methanothermobacter marburgensis</em> in a biological methane production process (BMPP) |
| title_full | Experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of <em>Methanothermobacter marburgensis</em> in a biological methane production process (BMPP) |
| title_fullStr | Experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of <em>Methanothermobacter marburgensis</em> in a biological methane production process (BMPP) |
| title_full_unstemmed | Experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of <em>Methanothermobacter marburgensis</em> in a biological methane production process (BMPP) |
| title_short | Experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of <em>Methanothermobacter marburgensis</em> in a biological methane production process (BMPP) |
| title_sort | experimental workflow for developing a feed forward strategy to control biomass growth and exploit maximum specific methane productivity of em methanothermobacter marburgensis em in a biological methane production process bmpp |
| topic | design of experiments chemostat bioprocess quantification carbon balance biological methanogenesis gas limited bioprocess continuous bioprocess power to gas liquid limited growth waste to value |
| url | http://www.aimspress.com/microbiology/article/900/fulltext.html |
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