Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon Tissue
The risk of the development of tendon disorders or ruptures increases with age, but it is unclear whether intrinsic fitness during lifetime might also affect tendon properties. To investigate this, a contrasting rat model of high-capacity runners (HCR with high intrinsic fitness) and low-capacity ru...
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| Format: | Article |
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MDPI AG
2022-02-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/10/2/509 |
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| author | Manuela Thierbach Estelle Heyne Michael Schwarzer Lauren G. Koch Steven L. Britton Britt Wildemann |
| author_facet | Manuela Thierbach Estelle Heyne Michael Schwarzer Lauren G. Koch Steven L. Britton Britt Wildemann |
| author_sort | Manuela Thierbach |
| collection | DOAJ |
| description | The risk of the development of tendon disorders or ruptures increases with age, but it is unclear whether intrinsic fitness during lifetime might also affect tendon properties. To investigate this, a contrasting rat model of high-capacity runners (HCR with high intrinsic fitness) and low-capacity runners (LCR with low intrinsic fitness) was employed. Histological and molecular changes in rotator cuff (RC) tendons from 10 weeks old (young; HCR-10 and LCR-10) and 100 weeks old (old; HCR-100 and LCR-100) female rats were investigated. Age-dependent changes of RC tendons observed in HCR and LCR were increase of weight, decrease of tenocytes and RNA content, reduction of the wavy pattern of collagen and elastic fibers, repressed expression of <i>Col1a1</i>, <i>Eln</i>, <i>Postn</i>, <i>Tnmd</i>, <i>Tgfb3</i> and <i>Egr1</i> and reduction of the <i>Col1</i>:<i>Col3</i> and <i>Col1</i>:<i>Eln</i> ratio. The LCR rats showed less physical activity, increased body weight, signs of metabolic disease and a reduced life expectancy. Their RC tendons revealed increased weight (more than age-dependent) and enlargement of the tenocyte nuclei (consistent with degenerative tendons). Low intrinsic fitness led to repressed expression of a further nine genes (<i>Col3a1</i>, <i>Fbn1</i>, <i>Dcn</i>, <i>Tnc</i>, <i>Scx</i>, <i>Mkx</i>, <i>Bmp1</i>, <i>Tgfb1</i>, <i>Esr1</i>) as well as the rise of the <i>Col1</i>:<i>Col3</i> and <i>Col1:Eln</i> ratios (related to the lesser expression of <i>Col3a1</i> and <i>Eln</i>). The intrinsic fitness influences the female RC tendons at least as much as age. Lower intrinsic fitness accelerates aging of RC tendons and leads to further impairment; this could result in decreased healing potential and elasticity and increased stiffness. |
| first_indexed | 2024-03-09T22:30:33Z |
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| id | doaj.art-0b456990f8644a00b8b28829d414c568 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T22:30:33Z |
| publishDate | 2022-02-01 |
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| spelling | doaj.art-0b456990f8644a00b8b28829d414c5682023-11-23T18:56:43ZengMDPI AGBiomedicines2227-90592022-02-0110250910.3390/biomedicines10020509Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon TissueManuela Thierbach0Estelle Heyne1Michael Schwarzer2Lauren G. Koch3Steven L. Britton4Britt Wildemann5Experimental Trauma Surgery, Department of Trauma, Hand and Reconstructive Surgery, Jena University Hospital, Friedrich Schiller University Jena, 07747 Jena, GermanyDepartment of Cardiothoracic Surgery, Jena University Hospital, 07747 Jena, GermanyDepartment of Cardiothoracic Surgery, Jena University Hospital, 07747 Jena, GermanyDepartment of Physiology and Pharmacology, The University of Toledo, Toledo, OH 43606, USADepartment of Molecular and Integrative Physiology, University of Michigan, Ann Arbor, MI 48109, USAExperimental Trauma Surgery, Department of Trauma, Hand and Reconstructive Surgery, Jena University Hospital, Friedrich Schiller University Jena, 07747 Jena, GermanyThe risk of the development of tendon disorders or ruptures increases with age, but it is unclear whether intrinsic fitness during lifetime might also affect tendon properties. To investigate this, a contrasting rat model of high-capacity runners (HCR with high intrinsic fitness) and low-capacity runners (LCR with low intrinsic fitness) was employed. Histological and molecular changes in rotator cuff (RC) tendons from 10 weeks old (young; HCR-10 and LCR-10) and 100 weeks old (old; HCR-100 and LCR-100) female rats were investigated. Age-dependent changes of RC tendons observed in HCR and LCR were increase of weight, decrease of tenocytes and RNA content, reduction of the wavy pattern of collagen and elastic fibers, repressed expression of <i>Col1a1</i>, <i>Eln</i>, <i>Postn</i>, <i>Tnmd</i>, <i>Tgfb3</i> and <i>Egr1</i> and reduction of the <i>Col1</i>:<i>Col3</i> and <i>Col1</i>:<i>Eln</i> ratio. The LCR rats showed less physical activity, increased body weight, signs of metabolic disease and a reduced life expectancy. Their RC tendons revealed increased weight (more than age-dependent) and enlargement of the tenocyte nuclei (consistent with degenerative tendons). Low intrinsic fitness led to repressed expression of a further nine genes (<i>Col3a1</i>, <i>Fbn1</i>, <i>Dcn</i>, <i>Tnc</i>, <i>Scx</i>, <i>Mkx</i>, <i>Bmp1</i>, <i>Tgfb1</i>, <i>Esr1</i>) as well as the rise of the <i>Col1</i>:<i>Col3</i> and <i>Col1:Eln</i> ratios (related to the lesser expression of <i>Col3a1</i> and <i>Eln</i>). The intrinsic fitness influences the female RC tendons at least as much as age. Lower intrinsic fitness accelerates aging of RC tendons and leads to further impairment; this could result in decreased healing potential and elasticity and increased stiffness.https://www.mdpi.com/2227-9059/10/2/509tendonhigh-capacity runners (HCR)low-capacity runners (LCR)rotator cuffhistologygene expression |
| spellingShingle | Manuela Thierbach Estelle Heyne Michael Schwarzer Lauren G. Koch Steven L. Britton Britt Wildemann Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon Tissue Biomedicines tendon high-capacity runners (HCR) low-capacity runners (LCR) rotator cuff histology gene expression |
| title | Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon Tissue |
| title_full | Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon Tissue |
| title_fullStr | Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon Tissue |
| title_full_unstemmed | Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon Tissue |
| title_short | Age and Intrinsic Fitness Affect the Female Rotator Cuff Tendon Tissue |
| title_sort | age and intrinsic fitness affect the female rotator cuff tendon tissue |
| topic | tendon high-capacity runners (HCR) low-capacity runners (LCR) rotator cuff histology gene expression |
| url | https://www.mdpi.com/2227-9059/10/2/509 |
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