Cloning of Metalloproteinase 17 Genes from Oriental Giant Jellyfish <i>Nemopilema nomurai</i> (Scyphozoa: Rhizostomeae)
We previously demonstrated that <i>Nemopilema nomurai</i> jellyfish venom metalloproteinases (JVMPs) play a key role in the toxicities induced by <i>N. nomurai</i> venom (NnV), including dermotoxicity, cytotoxicity, and lethality. In this study, we identified two full-length...
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MDPI AG
2022-07-01
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| author | Du Hyeon Hwang Yunwi Heo Young Chul Kwon Ramachandran Loganathan Mohan Prakash Kyoungyeon Kim Hyunju Oh Ramin Seyedian Al Munawir Changkeun Kang Euikyung Kim |
| author_facet | Du Hyeon Hwang Yunwi Heo Young Chul Kwon Ramachandran Loganathan Mohan Prakash Kyoungyeon Kim Hyunju Oh Ramin Seyedian Al Munawir Changkeun Kang Euikyung Kim |
| author_sort | Du Hyeon Hwang |
| collection | DOAJ |
| description | We previously demonstrated that <i>Nemopilema nomurai</i> jellyfish venom metalloproteinases (JVMPs) play a key role in the toxicities induced by <i>N. nomurai</i> venom (NnV), including dermotoxicity, cytotoxicity, and lethality. In this study, we identified two full-length JVMP cDNA and genomic DNA sequences: JVMP17-1 and JVMP17-2. The full-length cDNA of JVMP17-1 and 17-2 contains 1614 and 1578 nucleotides (nt) that encode 536 and 525 amino acids, respectively. Putative peptidoglycan (PG) binding, zinc-dependent metalloproteinase, and hemopexin domains were identified. BLAST analysis of JVMP17-1 showed 42, 41, 37, and 37% identity with <i>Hydra vulgaris</i>, <i>Acropora digitifera</i>, <i>Megachile rotundata,</i> and <i>Apis mellifera</i> venom metalloproteinases, respectively. JVMP17-2 shared 38 and 36% identity with <i>H. vulgaris</i> and <i>A</i>. <i>digitifera</i>, respectively. Alignment results of JVMP17-1 and 17-2 with other metalloproteinases suggest that the PG domain, the tissue inhibitor of metalloproteinase (TIMP)-binding surfaces, active sites, and metal (ion)-binding sites are highly conserved. The present study reports the gene cloning of metalloproteinase enzymes from jellyfish species for the first time. We hope these results can expand our knowledge of metalloproteinase components and their roles in the pathogenesis of jellyfish envenomation. |
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| spelling | doaj.art-0b477d215e5d47c3bcb096a3c47df9f82023-12-03T14:35:17ZengMDPI AGToxins2072-66512022-07-0114851910.3390/toxins14080519Cloning of Metalloproteinase 17 Genes from Oriental Giant Jellyfish <i>Nemopilema nomurai</i> (Scyphozoa: Rhizostomeae)Du Hyeon Hwang0Yunwi Heo1Young Chul Kwon2Ramachandran Loganathan Mohan Prakash3Kyoungyeon Kim4Hyunju Oh5Ramin Seyedian6Al Munawir7Changkeun Kang8Euikyung Kim9Department of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaDepartment of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaDepartment of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaDepartment of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaOcean Climate & Ecology Research Division, National Institute of Fisheries Science, Busan 46083, KoreaOcean Climate & Ecology Research Division, National Institute of Fisheries Science, Busan 46083, KoreaDepartment of Pharmacology, Bushehr University of Medical Sciences, Bushehr 14174, IranPathology Laboratory, Medical Faculty, University of Jember, Jember 68126, IndonesiaDepartment of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaDepartment of Pharmacology and Toxicology, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, KoreaWe previously demonstrated that <i>Nemopilema nomurai</i> jellyfish venom metalloproteinases (JVMPs) play a key role in the toxicities induced by <i>N. nomurai</i> venom (NnV), including dermotoxicity, cytotoxicity, and lethality. In this study, we identified two full-length JVMP cDNA and genomic DNA sequences: JVMP17-1 and JVMP17-2. The full-length cDNA of JVMP17-1 and 17-2 contains 1614 and 1578 nucleotides (nt) that encode 536 and 525 amino acids, respectively. Putative peptidoglycan (PG) binding, zinc-dependent metalloproteinase, and hemopexin domains were identified. BLAST analysis of JVMP17-1 showed 42, 41, 37, and 37% identity with <i>Hydra vulgaris</i>, <i>Acropora digitifera</i>, <i>Megachile rotundata,</i> and <i>Apis mellifera</i> venom metalloproteinases, respectively. JVMP17-2 shared 38 and 36% identity with <i>H. vulgaris</i> and <i>A</i>. <i>digitifera</i>, respectively. Alignment results of JVMP17-1 and 17-2 with other metalloproteinases suggest that the PG domain, the tissue inhibitor of metalloproteinase (TIMP)-binding surfaces, active sites, and metal (ion)-binding sites are highly conserved. The present study reports the gene cloning of metalloproteinase enzymes from jellyfish species for the first time. We hope these results can expand our knowledge of metalloproteinase components and their roles in the pathogenesis of jellyfish envenomation.https://www.mdpi.com/2072-6651/14/8/519jellyfish venommetalloproteinasesequencing |
| spellingShingle | Du Hyeon Hwang Yunwi Heo Young Chul Kwon Ramachandran Loganathan Mohan Prakash Kyoungyeon Kim Hyunju Oh Ramin Seyedian Al Munawir Changkeun Kang Euikyung Kim Cloning of Metalloproteinase 17 Genes from Oriental Giant Jellyfish <i>Nemopilema nomurai</i> (Scyphozoa: Rhizostomeae) Toxins jellyfish venom metalloproteinase sequencing |
| title | Cloning of Metalloproteinase 17 Genes from Oriental Giant Jellyfish <i>Nemopilema nomurai</i> (Scyphozoa: Rhizostomeae) |
| title_full | Cloning of Metalloproteinase 17 Genes from Oriental Giant Jellyfish <i>Nemopilema nomurai</i> (Scyphozoa: Rhizostomeae) |
| title_fullStr | Cloning of Metalloproteinase 17 Genes from Oriental Giant Jellyfish <i>Nemopilema nomurai</i> (Scyphozoa: Rhizostomeae) |
| title_full_unstemmed | Cloning of Metalloproteinase 17 Genes from Oriental Giant Jellyfish <i>Nemopilema nomurai</i> (Scyphozoa: Rhizostomeae) |
| title_short | Cloning of Metalloproteinase 17 Genes from Oriental Giant Jellyfish <i>Nemopilema nomurai</i> (Scyphozoa: Rhizostomeae) |
| title_sort | cloning of metalloproteinase 17 genes from oriental giant jellyfish i nemopilema nomurai i scyphozoa rhizostomeae |
| topic | jellyfish venom metalloproteinase sequencing |
| url | https://www.mdpi.com/2072-6651/14/8/519 |
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