Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloes
Present study was undertaken to assess AMH concentration in serum and histopathological study of AFC in buffalo ovaries. Total 30 pair of ovary were collected in 10% Neutral Buffer Formalin. Blood samples were also collected before slaughtering the buffalo animals for estimation of AMH concentratio...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2020-09-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/104201 |
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| author | P M KEKAN S D INGOLE A S NAVEKAR S V BHARUCHA S D KHARDE |
| author_facet | P M KEKAN S D INGOLE A S NAVEKAR S V BHARUCHA S D KHARDE |
| author_sort | P M KEKAN |
| collection | DOAJ |
| description |
Present study was undertaken to assess AMH concentration in serum and histopathological study of AFC in buffalo ovaries. Total 30 pair of ovary were collected in 10% Neutral Buffer Formalin. Blood samples were also collected before slaughtering the buffalo animals for estimation of AMH concentration. Before processing, 10 serial sections of each pair of ovary was done for histopathological examinations. All the serial sections of the ovaries were processed as per the standard procedure. On the basis of histopathological examinations and follicle count, animals were categorized as cyclic and anestrus. Out of 30 pairs of ovary, 18 pairs were identified as anestrus and 12 as cyclic. The mean number of primordial, antral and growing ovarian follicles count of cyclic buffaloes were 0.42±0.07, 0.15±0.03 and 4.47±0.03 and 0.49±0.02, 0.16±0.03 and 0.41±0.06 for anestrus buffaloes, respectively. Growing follicles were significantly higher in cyclic as compared to anestrous buffaloes. However, the difference of primordial and antral follicle count remained non-significant in cyclic and anestrous buffaloes. Whereas, AMH concentration in cyclic buffaloes (273.50±48.52) was significantly higher than anestrous buffaloes (79.40±7.80). Therefore, it is concluded that, anti-mullerian hormone is an endocrine marker and can predict the fertility of animal. Also, follicular count is positively correlated with AMH concentration.
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| format | Article |
| id | doaj.art-0b49af3a7ccc4cf4956160d011c7c4c5 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:47:17Z |
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| publisher | Indian Council of Agricultural Research |
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| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-0b49af3a7ccc4cf4956160d011c7c4c52023-02-23T10:24:33ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272020-09-0190410.56093/ijans.v90i4.104201Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloesP M KEKAN0S D INGOLE1A S NAVEKAR2S V BHARUCHA3S D KHARDE4Bombay Veterinary College, Mumbai, Maharashtra 400 012 IndiaBombay Veterinary College, Mumbai, Maharashtra 400 012 IndiaBombay Veterinary College, Mumbai, Maharashtra 400 012 IndiaBombay Veterinary College, Mumbai, Maharashtra 400 012 IndiaBombay Veterinary College, Mumbai, Maharashtra 400 012 India Present study was undertaken to assess AMH concentration in serum and histopathological study of AFC in buffalo ovaries. Total 30 pair of ovary were collected in 10% Neutral Buffer Formalin. Blood samples were also collected before slaughtering the buffalo animals for estimation of AMH concentration. Before processing, 10 serial sections of each pair of ovary was done for histopathological examinations. All the serial sections of the ovaries were processed as per the standard procedure. On the basis of histopathological examinations and follicle count, animals were categorized as cyclic and anestrus. Out of 30 pairs of ovary, 18 pairs were identified as anestrus and 12 as cyclic. The mean number of primordial, antral and growing ovarian follicles count of cyclic buffaloes were 0.42±0.07, 0.15±0.03 and 4.47±0.03 and 0.49±0.02, 0.16±0.03 and 0.41±0.06 for anestrus buffaloes, respectively. Growing follicles were significantly higher in cyclic as compared to anestrous buffaloes. However, the difference of primordial and antral follicle count remained non-significant in cyclic and anestrous buffaloes. Whereas, AMH concentration in cyclic buffaloes (273.50±48.52) was significantly higher than anestrous buffaloes (79.40±7.80). Therefore, it is concluded that, anti-mullerian hormone is an endocrine marker and can predict the fertility of animal. Also, follicular count is positively correlated with AMH concentration. https://epubs.icar.org.in/index.php/IJAnS/article/view/104201AnestrousAnti-Mulleian hormoneBuffaloesCattleHistopathologyOvary |
| spellingShingle | P M KEKAN S D INGOLE A S NAVEKAR S V BHARUCHA S D KHARDE Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloes Indian Journal of Animal Sciences Anestrous Anti-Mulleian hormone Buffaloes Cattle Histopathology Ovary |
| title | Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloes |
| title_full | Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloes |
| title_fullStr | Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloes |
| title_full_unstemmed | Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloes |
| title_short | Determination of anti-mullerian hormone in serum and ovarian histopathology of buffaloes |
| title_sort | determination of anti mullerian hormone in serum and ovarian histopathology of buffaloes |
| topic | Anestrous Anti-Mulleian hormone Buffaloes Cattle Histopathology Ovary |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/104201 |
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