MICROPROPAGATION OF ROSA DAMASCENA MILL.: THE EFFECTS OF GELLING AGENTS ON THE MULTIPLICATION STAGES AND ACCLIMATIZATION

The aim of our research was to investigate the way in which the agar types used for culture media preparation influence damask rose in vitro proliferation rates, as well as the quality of the shoots obtained and their ability for ex vitro rooting and acclimatization. For this purpose, seven types of...

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Main Authors: Doina Clapa, Alexandru Fira, Orsolya Borsai, Monica Hârța, Cristian Sisea, Rodica Pop, Doru Pamfil
Format: Article
Language:English
Published: AcademicPres 2017-11-01
Series:Agricultura
Subjects:
Online Access:https://journals.usamvcluj.ro/index.php/agricultura/article/view/12830
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author Doina Clapa
Alexandru Fira
Orsolya Borsai
Monica Hârța
Cristian Sisea
Rodica Pop
Doru Pamfil
author_facet Doina Clapa
Alexandru Fira
Orsolya Borsai
Monica Hârța
Cristian Sisea
Rodica Pop
Doru Pamfil
author_sort Doina Clapa
collection DOAJ
description The aim of our research was to investigate the way in which the agar types used for culture media preparation influence damask rose in vitro proliferation rates, as well as the quality of the shoots obtained and their ability for ex vitro rooting and acclimatization. For this purpose, seven types of gelling agents were tested: 6 g/L Daishin Agar, 4 g/L Gelcarin, 2 g/L Gelrite, 15 g/L Isubgol, 4 g/L Micro Agar, 6 g/L Phyto Agar and 4 g/L Plant Agar. The culture media consisted of Murashige & Skoog supplemented with 4 mg/L 6-benzyladenine. The highest multiplication rate, 5.1, was obtained on the culture media supplemented with 6g/L Daishin Agar and 4 g/L Plant Agar and the lowest one was 2.8, obtained on the media gelled with 4 g/L Gelcarin. Although the multiplication rate on the media gelled with Isubgol was lower (3.2), the shoots obtained in this experimental treatment got acclimatized with 90 % survival percentage and developed the best root system, regarding the number of roots/shoot and root length.
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spelling doaj.art-0b4c5d88b53e4fe68f7564ed9003169b2022-12-21T20:16:40ZengAcademicPresAgricultura1221-53172017-11-011033-410.15835/agrisp.v103i3-4.1283010478MICROPROPAGATION OF ROSA DAMASCENA MILL.: THE EFFECTS OF GELLING AGENTS ON THE MULTIPLICATION STAGES AND ACCLIMATIZATIONDoina Clapa0Alexandru FiraOrsolya BorsaiMonica HârțaCristian SiseaRodica PopDoru PamfilUniversity of Agricultural Science and Veterinary Medicine, Cluj-NapocaThe aim of our research was to investigate the way in which the agar types used for culture media preparation influence damask rose in vitro proliferation rates, as well as the quality of the shoots obtained and their ability for ex vitro rooting and acclimatization. For this purpose, seven types of gelling agents were tested: 6 g/L Daishin Agar, 4 g/L Gelcarin, 2 g/L Gelrite, 15 g/L Isubgol, 4 g/L Micro Agar, 6 g/L Phyto Agar and 4 g/L Plant Agar. The culture media consisted of Murashige & Skoog supplemented with 4 mg/L 6-benzyladenine. The highest multiplication rate, 5.1, was obtained on the culture media supplemented with 6g/L Daishin Agar and 4 g/L Plant Agar and the lowest one was 2.8, obtained on the media gelled with 4 g/L Gelcarin. Although the multiplication rate on the media gelled with Isubgol was lower (3.2), the shoots obtained in this experimental treatment got acclimatized with 90 % survival percentage and developed the best root system, regarding the number of roots/shoot and root length.https://journals.usamvcluj.ro/index.php/agricultura/article/view/12830daishin agar, gelcarin, gelrite, isubgol, micro agar, phyto agar, plant agar
spellingShingle Doina Clapa
Alexandru Fira
Orsolya Borsai
Monica Hârța
Cristian Sisea
Rodica Pop
Doru Pamfil
MICROPROPAGATION OF ROSA DAMASCENA MILL.: THE EFFECTS OF GELLING AGENTS ON THE MULTIPLICATION STAGES AND ACCLIMATIZATION
Agricultura
daishin agar, gelcarin, gelrite, isubgol, micro agar, phyto agar, plant agar
title MICROPROPAGATION OF ROSA DAMASCENA MILL.: THE EFFECTS OF GELLING AGENTS ON THE MULTIPLICATION STAGES AND ACCLIMATIZATION
title_full MICROPROPAGATION OF ROSA DAMASCENA MILL.: THE EFFECTS OF GELLING AGENTS ON THE MULTIPLICATION STAGES AND ACCLIMATIZATION
title_fullStr MICROPROPAGATION OF ROSA DAMASCENA MILL.: THE EFFECTS OF GELLING AGENTS ON THE MULTIPLICATION STAGES AND ACCLIMATIZATION
title_full_unstemmed MICROPROPAGATION OF ROSA DAMASCENA MILL.: THE EFFECTS OF GELLING AGENTS ON THE MULTIPLICATION STAGES AND ACCLIMATIZATION
title_short MICROPROPAGATION OF ROSA DAMASCENA MILL.: THE EFFECTS OF GELLING AGENTS ON THE MULTIPLICATION STAGES AND ACCLIMATIZATION
title_sort micropropagation of rosa damascena mill the effects of gelling agents on the multiplication stages and acclimatization
topic daishin agar, gelcarin, gelrite, isubgol, micro agar, phyto agar, plant agar
url https://journals.usamvcluj.ro/index.php/agricultura/article/view/12830
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