A protein secreted by the Salmonella type III secretion system controls needle filament assembly
Type III protein secretion systems (T3SS) are encoded by several pathogenic or symbiotic bacteria. The central component of this nanomachine is the needle complex. Here we show in a Salmonella Typhimurium T3SS that assembly of the needle filament of this structure requires OrgC, a protein encoded wi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2018-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/35886 |
| _version_ | 1811253104207724544 |
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| author | Junya Kato Supratim Dey Jose E Soto Carmen Butan Mason C Wilkinson Roberto N De Guzman Jorge E Galan |
| author_facet | Junya Kato Supratim Dey Jose E Soto Carmen Butan Mason C Wilkinson Roberto N De Guzman Jorge E Galan |
| author_sort | Junya Kato |
| collection | DOAJ |
| description | Type III protein secretion systems (T3SS) are encoded by several pathogenic or symbiotic bacteria. The central component of this nanomachine is the needle complex. Here we show in a Salmonella Typhimurium T3SS that assembly of the needle filament of this structure requires OrgC, a protein encoded within the T3SS gene cluster. Absence of OrgC results in significantly reduced number of needle substructures but does not affect needle length. We show that OrgC is secreted by the T3SS and that exogenous addition of OrgC can complement a ∆orgC mutation. We also show that OrgC interacts with the needle filament subunit PrgI and accelerates its polymerization into filaments in vitro. The structure of OrgC shows a novel fold with a shared topology with a domain from flagellar capping proteins. These findings identify a novel component of T3SS and provide new insight into the assembly of the type III secretion machine. |
| first_indexed | 2024-04-12T16:44:49Z |
| format | Article |
| id | doaj.art-0b4e2ae158644ceb800dce215a709b3c |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T16:44:49Z |
| publishDate | 2018-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-0b4e2ae158644ceb800dce215a709b3c2022-12-22T03:24:36ZengeLife Sciences Publications LtdeLife2050-084X2018-07-01710.7554/eLife.35886A protein secreted by the Salmonella type III secretion system controls needle filament assemblyJunya Kato0Supratim Dey1Jose E Soto2Carmen Butan3Mason C Wilkinson4Roberto N De Guzman5Jorge E Galan6https://orcid.org/0000-0002-6531-0355Department of Microbial Pathogenesis, Yale University School of Medicine, New Haven, United StatesDepartment of Molecular Biosciences, University of Kansas, Lawrence, United StatesDepartment of Microbial Pathogenesis, Yale University School of Medicine, New Haven, United StatesDepartment of Microbial Pathogenesis, Yale University School of Medicine, New Haven, United StatesDepartment of Molecular Biosciences, University of Kansas, Lawrence, United StatesDepartment of Molecular Biosciences, University of Kansas, Lawrence, United StatesDepartment of Microbial Pathogenesis, Yale University School of Medicine, New Haven, United StatesType III protein secretion systems (T3SS) are encoded by several pathogenic or symbiotic bacteria. The central component of this nanomachine is the needle complex. Here we show in a Salmonella Typhimurium T3SS that assembly of the needle filament of this structure requires OrgC, a protein encoded within the T3SS gene cluster. Absence of OrgC results in significantly reduced number of needle substructures but does not affect needle length. We show that OrgC is secreted by the T3SS and that exogenous addition of OrgC can complement a ∆orgC mutation. We also show that OrgC interacts with the needle filament subunit PrgI and accelerates its polymerization into filaments in vitro. The structure of OrgC shows a novel fold with a shared topology with a domain from flagellar capping proteins. These findings identify a novel component of T3SS and provide new insight into the assembly of the type III secretion machine.https://elifesciences.org/articles/35886protien secretionbacterial pathogenesissalmonella typhimuriumtype III secretionbacterial nanomachinesorganelle assembly |
| spellingShingle | Junya Kato Supratim Dey Jose E Soto Carmen Butan Mason C Wilkinson Roberto N De Guzman Jorge E Galan A protein secreted by the Salmonella type III secretion system controls needle filament assembly eLife protien secretion bacterial pathogenesis salmonella typhimurium type III secretion bacterial nanomachines organelle assembly |
| title | A protein secreted by the Salmonella type III secretion system controls needle filament assembly |
| title_full | A protein secreted by the Salmonella type III secretion system controls needle filament assembly |
| title_fullStr | A protein secreted by the Salmonella type III secretion system controls needle filament assembly |
| title_full_unstemmed | A protein secreted by the Salmonella type III secretion system controls needle filament assembly |
| title_short | A protein secreted by the Salmonella type III secretion system controls needle filament assembly |
| title_sort | protein secreted by the salmonella type iii secretion system controls needle filament assembly |
| topic | protien secretion bacterial pathogenesis salmonella typhimurium type III secretion bacterial nanomachines organelle assembly |
| url | https://elifesciences.org/articles/35886 |
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