Successful fertilization and embryo development after spermatid injection: A hope for nonobstructive azoospermic patients
Spermatids are the earliest male germ cells with haploid set of chromosomes. Spermatid injection was introduced in human assisted reproduction for the treatment of men with non-obstructive azoospermia. Spermatozoa can be recovered in half of patients with nonobstructive azoospermia. The use of sperm...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2015-01-01
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| Series: | Journal of Human Reproductive Sciences |
| Subjects: | |
| Online Access: | http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2015;volume=8;issue=3;spage=175;epage=177;aulast=Goswami |
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| author | Geeta Goswami Sarabjeet Singh M Gouri Devi |
| author_facet | Geeta Goswami Sarabjeet Singh M Gouri Devi |
| author_sort | Geeta Goswami |
| collection | DOAJ |
| description | Spermatids are the earliest male germ cells with haploid set of chromosomes. Spermatid injection was introduced in human assisted reproduction for the treatment of men with non-obstructive azoospermia. Spermatozoa can be recovered in half of patients with nonobstructive azoospermia. The use of spermatids for intracytoplasmic injection (ICSI) has been proposed for cases in which no spermatozoa can be retrieved. However, there are low pregnancy rates following ICSI using round spermatids from men with no elongated spermatids or spermatozoa in their testes. The in vitroculture of immature germ cells has been proposed as a means to improve this poor outcome. Oocyte activation rarely occurs when injected with a spermatid. Therefore, spermatid injection requires use of calcium ionophores for oocyte activation which is otherwise carried out by PLC zeta from mature sperms. This is the only option available for the nonobstructive azoospermic patients to have their own biological child. |
| first_indexed | 2024-04-13T16:17:47Z |
| format | Article |
| id | doaj.art-0b505b2f5c524071bf56f6a3b316fcdf |
| institution | Directory Open Access Journal |
| issn | 0974-1208 1998-4766 |
| language | English |
| last_indexed | 2024-04-13T16:17:47Z |
| publishDate | 2015-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Human Reproductive Sciences |
| spelling | doaj.art-0b505b2f5c524071bf56f6a3b316fcdf2022-12-22T02:40:00ZengWolters Kluwer Medknow PublicationsJournal of Human Reproductive Sciences0974-12081998-47662015-01-018317517710.4103/0974-1208.165147Successful fertilization and embryo development after spermatid injection: A hope for nonobstructive azoospermic patientsGeeta GoswamiSarabjeet SinghM Gouri DeviSpermatids are the earliest male germ cells with haploid set of chromosomes. Spermatid injection was introduced in human assisted reproduction for the treatment of men with non-obstructive azoospermia. Spermatozoa can be recovered in half of patients with nonobstructive azoospermia. The use of spermatids for intracytoplasmic injection (ICSI) has been proposed for cases in which no spermatozoa can be retrieved. However, there are low pregnancy rates following ICSI using round spermatids from men with no elongated spermatids or spermatozoa in their testes. The in vitroculture of immature germ cells has been proposed as a means to improve this poor outcome. Oocyte activation rarely occurs when injected with a spermatid. Therefore, spermatid injection requires use of calcium ionophores for oocyte activation which is otherwise carried out by PLC zeta from mature sperms. This is the only option available for the nonobstructive azoospermic patients to have their own biological child.http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2015;volume=8;issue=3;spage=175;epage=177;aulast=GoswamiIntracytoplasmic sperm injectionnonobstructive azoospermiaoocyte activationround spermatids |
| spellingShingle | Geeta Goswami Sarabjeet Singh M Gouri Devi Successful fertilization and embryo development after spermatid injection: A hope for nonobstructive azoospermic patients Journal of Human Reproductive Sciences Intracytoplasmic sperm injection nonobstructive azoospermia oocyte activation round spermatids |
| title | Successful fertilization and embryo development after spermatid injection: A hope for nonobstructive azoospermic patients |
| title_full | Successful fertilization and embryo development after spermatid injection: A hope for nonobstructive azoospermic patients |
| title_fullStr | Successful fertilization and embryo development after spermatid injection: A hope for nonobstructive azoospermic patients |
| title_full_unstemmed | Successful fertilization and embryo development after spermatid injection: A hope for nonobstructive azoospermic patients |
| title_short | Successful fertilization and embryo development after spermatid injection: A hope for nonobstructive azoospermic patients |
| title_sort | successful fertilization and embryo development after spermatid injection a hope for nonobstructive azoospermic patients |
| topic | Intracytoplasmic sperm injection nonobstructive azoospermia oocyte activation round spermatids |
| url | http://www.jhrsonline.org/article.asp?issn=0974-1208;year=2015;volume=8;issue=3;spage=175;epage=177;aulast=Goswami |
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