Surviving within the amoebal exocyst: the <it>Mycobacterium avium </it>complex paradigm
<p>Abstract</p> <p>Background</p> <p>Most of environmental mycobacteria have been previously demonstrated to resist free-living amoeba with subsequent increased virulence and resistance to antibiotics and biocides. The <it>Mycobacterium avium </it>complex (M...
| Huvudupphovsmän: | , |
|---|---|
| Materialtyp: | Artikel |
| Språk: | English |
| Publicerad: |
BMC
2010-04-01
|
| Serie: | BMC Microbiology |
| Länkar: | http://www.biomedcentral.com/1471-2180/10/99 |
| _version_ | 1828952290495561728 |
|---|---|
| author | Drancourt Michel Ben Salah Iskandar |
| author_facet | Drancourt Michel Ben Salah Iskandar |
| author_sort | Drancourt Michel |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Most of environmental mycobacteria have been previously demonstrated to resist free-living amoeba with subsequent increased virulence and resistance to antibiotics and biocides. The <it>Mycobacterium avium </it>complex (MAC) comprises of environmental organisms that inhabit a wide variety of ecological niches and exhibit a significant degree of genetic variability. We herein studied the intra-ameobal location of all members of the MAC as model organisms for environmental mycobacteria.</p> <p>Results</p> <p>Type strains for <it>M. avium</it>, <it>Mycobacterium intracellulare</it>, <it>Mycobacterium chimaera</it>, <it>Mycobacterium colombiense</it>, <it>Mycobacterium arosiense</it>, <it>Mycobacterium marseillense</it>, <it>Mycobacterium timonense </it>and <it>Mycobacterium bouchedurhonense </it>were co-cultivated with the free-living amoeba <it>Acanthamoeba polyphaga </it>strain Linc-AP1. Microscopic analyses demonstrated the engulfment and replication of mycobacteria into vacuoles of <it>A. polyphaga </it>trophozoites. Mycobacteria were further entrapped within amoebal cysts, and survived encystment as demonstrated by subculturing. Electron microscopy observations show that, three days after entrapment into <it>A. polyphaga </it>cysts, all MAC members typically resided within the exocyst.</p> <p>Conclusions</p> <p>Combined with published data, these observations indicate that mycobacteria are unique among amoeba-resistant bacteria, in residing within the exocyst.</p> |
| first_indexed | 2024-12-14T06:50:18Z |
| format | Article |
| id | doaj.art-0b51f710e39d42318dba4f16e7e8d6a5 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-14T06:50:18Z |
| publishDate | 2010-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-0b51f710e39d42318dba4f16e7e8d6a52022-12-21T23:12:54ZengBMCBMC Microbiology1471-21802010-04-011019910.1186/1471-2180-10-99Surviving within the amoebal exocyst: the <it>Mycobacterium avium </it>complex paradigmDrancourt MichelBen Salah Iskandar<p>Abstract</p> <p>Background</p> <p>Most of environmental mycobacteria have been previously demonstrated to resist free-living amoeba with subsequent increased virulence and resistance to antibiotics and biocides. The <it>Mycobacterium avium </it>complex (MAC) comprises of environmental organisms that inhabit a wide variety of ecological niches and exhibit a significant degree of genetic variability. We herein studied the intra-ameobal location of all members of the MAC as model organisms for environmental mycobacteria.</p> <p>Results</p> <p>Type strains for <it>M. avium</it>, <it>Mycobacterium intracellulare</it>, <it>Mycobacterium chimaera</it>, <it>Mycobacterium colombiense</it>, <it>Mycobacterium arosiense</it>, <it>Mycobacterium marseillense</it>, <it>Mycobacterium timonense </it>and <it>Mycobacterium bouchedurhonense </it>were co-cultivated with the free-living amoeba <it>Acanthamoeba polyphaga </it>strain Linc-AP1. Microscopic analyses demonstrated the engulfment and replication of mycobacteria into vacuoles of <it>A. polyphaga </it>trophozoites. Mycobacteria were further entrapped within amoebal cysts, and survived encystment as demonstrated by subculturing. Electron microscopy observations show that, three days after entrapment into <it>A. polyphaga </it>cysts, all MAC members typically resided within the exocyst.</p> <p>Conclusions</p> <p>Combined with published data, these observations indicate that mycobacteria are unique among amoeba-resistant bacteria, in residing within the exocyst.</p>http://www.biomedcentral.com/1471-2180/10/99 |
| spellingShingle | Drancourt Michel Ben Salah Iskandar Surviving within the amoebal exocyst: the <it>Mycobacterium avium </it>complex paradigm BMC Microbiology |
| title | Surviving within the amoebal exocyst: the <it>Mycobacterium avium </it>complex paradigm |
| title_full | Surviving within the amoebal exocyst: the <it>Mycobacterium avium </it>complex paradigm |
| title_fullStr | Surviving within the amoebal exocyst: the <it>Mycobacterium avium </it>complex paradigm |
| title_full_unstemmed | Surviving within the amoebal exocyst: the <it>Mycobacterium avium </it>complex paradigm |
| title_short | Surviving within the amoebal exocyst: the <it>Mycobacterium avium </it>complex paradigm |
| title_sort | surviving within the amoebal exocyst the it mycobacterium avium it complex paradigm |
| url | http://www.biomedcentral.com/1471-2180/10/99 |
| work_keys_str_mv | AT drancourtmichel survivingwithintheamoebalexocysttheitmycobacteriumaviumitcomplexparadigm AT bensalahiskandar survivingwithintheamoebalexocysttheitmycobacteriumaviumitcomplexparadigm |