Endophytic fungi from Vitex payos: identification and bioactivity
Endophytic fungi isolated from medicinal plants have an important role to play in the search for new bioactive natural compounds. However, despite their potential as repositories of bioactive compounds, the endophytes of African medicinal plants are largely underexplored. The aim of this study was t...
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| Format: | Article |
| Language: | English |
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Polish Botanical Society
2018-12-01
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| Series: | Acta Mycologica |
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| Online Access: | https://pbsociety.org.pl/journals/index.php/am/article/view/8093 |
| _version_ | 1818720336555802624 |
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| author | Edson Panganayi Sibanda Musa Mabandla Tawanda Chisango Agness Farai Nhidza Takafira Mduluza |
| author_facet | Edson Panganayi Sibanda Musa Mabandla Tawanda Chisango Agness Farai Nhidza Takafira Mduluza |
| author_sort | Edson Panganayi Sibanda |
| collection | DOAJ |
| description | Endophytic fungi isolated from medicinal plants have an important role to play in the search for new bioactive natural compounds. However, despite their potential as repositories of bioactive compounds, the endophytes of African medicinal plants are largely underexplored. The aim of this study was to isolate and identify the endophytic fungi associated with Vitex payos and evaluate their antimicrobial and antioxidant potential. The surface sterilization technique was used to isolate the endophytic fungi that were identified by rDNA sequencing of the ITS region. Crude methanol and ethyl acetate extracts were screened for antimicrobial activity using the agar diffusion method and evaluated for antioxidant activity using a commercial total antioxidant capacity assay kit. The total phenolic content of the extracts was determined using the Folin–Ciocalteu method and functional groups present in the extracts were predicted using Fourier-transform infrared spectroscopy. Seven endophytic fungi isolates identified as Glomerella acutata, Epicoccum nigrum, Diaporthe species, Penicillium chloroleucon, Diaporthe endophytica, Mucor circinelloides, and Epicoccum nigrum were isolated from the tissues of Vitex payos. None of the extracts exhibited antimicrobial activity and the crude ethyl acetate extract obtained from E. nigrum demonstrated both the highest total phenolic content (2.97 ±0.13 mg GAE g−1 dry weight) and total antioxidant capacity (231.23 ±2.03 μM CRE). Fourier-transform infrared spectral analysis of the crude extracts from E. nigrum confirmed the presence of molecules carrying bonded hydroxyl functional group characteristic of phenolic compounds. These preliminary results indicate that most of the isolated fungal endophytes from V. payos belong to the phylum Ascomycota and that the isolated E. nigrum strain has potential as a source of natural antioxidants. |
| first_indexed | 2024-12-17T20:21:13Z |
| format | Article |
| id | doaj.art-0b535227eb844cd88d7d9a09269e2c9d |
| institution | Directory Open Access Journal |
| issn | 2353-074X |
| language | English |
| last_indexed | 2024-12-17T20:21:13Z |
| publishDate | 2018-12-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Mycologica |
| spelling | doaj.art-0b535227eb844cd88d7d9a09269e2c9d2022-12-21T21:33:56ZengPolish Botanical SocietyActa Mycologica2353-074X2018-12-0153210.5586/am.11116922Endophytic fungi from Vitex payos: identification and bioactivityEdson Panganayi Sibanda0Musa Mabandla1Tawanda Chisango2Agness Farai Nhidza3Takafira Mduluza4Scientific and Industrial Research and Development Centre, Food and Biomedical Technology Institute, 1574 Alpes Road/Scam Way, Harare; School of Laboratory Medicine and Medical Sciences, College of Health Sciences University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000, South AfricaSchool of Laboratory Medicine and Medical Sciences, College of Health Sciences University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000School of Laboratory Medicine and Medical Sciences, College of Health Sciences University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000School of Laboratory Medicine and Medical Sciences, College of Health Sciences University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000School of Laboratory Medicine and Medical Sciences, College of Health Sciences University of KwaZulu-Natal, Westville Campus, Private Bag X54001, Durban 4000; Department of Biochemistry, University of Zimbabwe, PO Box MP167 Mount Pleasant, Harare, ZimbabweEndophytic fungi isolated from medicinal plants have an important role to play in the search for new bioactive natural compounds. However, despite their potential as repositories of bioactive compounds, the endophytes of African medicinal plants are largely underexplored. The aim of this study was to isolate and identify the endophytic fungi associated with Vitex payos and evaluate their antimicrobial and antioxidant potential. The surface sterilization technique was used to isolate the endophytic fungi that were identified by rDNA sequencing of the ITS region. Crude methanol and ethyl acetate extracts were screened for antimicrobial activity using the agar diffusion method and evaluated for antioxidant activity using a commercial total antioxidant capacity assay kit. The total phenolic content of the extracts was determined using the Folin–Ciocalteu method and functional groups present in the extracts were predicted using Fourier-transform infrared spectroscopy. Seven endophytic fungi isolates identified as Glomerella acutata, Epicoccum nigrum, Diaporthe species, Penicillium chloroleucon, Diaporthe endophytica, Mucor circinelloides, and Epicoccum nigrum were isolated from the tissues of Vitex payos. None of the extracts exhibited antimicrobial activity and the crude ethyl acetate extract obtained from E. nigrum demonstrated both the highest total phenolic content (2.97 ±0.13 mg GAE g−1 dry weight) and total antioxidant capacity (231.23 ±2.03 μM CRE). Fourier-transform infrared spectral analysis of the crude extracts from E. nigrum confirmed the presence of molecules carrying bonded hydroxyl functional group characteristic of phenolic compounds. These preliminary results indicate that most of the isolated fungal endophytes from V. payos belong to the phylum Ascomycota and that the isolated E. nigrum strain has potential as a source of natural antioxidants.https://pbsociety.org.pl/journals/index.php/am/article/view/8093diversityantimicrobialantioxidantbioprospectingAfricaendophyte |
| spellingShingle | Edson Panganayi Sibanda Musa Mabandla Tawanda Chisango Agness Farai Nhidza Takafira Mduluza Endophytic fungi from Vitex payos: identification and bioactivity Acta Mycologica diversity antimicrobial antioxidant bioprospecting Africa endophyte |
| title | Endophytic fungi from Vitex payos: identification and bioactivity |
| title_full | Endophytic fungi from Vitex payos: identification and bioactivity |
| title_fullStr | Endophytic fungi from Vitex payos: identification and bioactivity |
| title_full_unstemmed | Endophytic fungi from Vitex payos: identification and bioactivity |
| title_short | Endophytic fungi from Vitex payos: identification and bioactivity |
| title_sort | endophytic fungi from vitex payos identification and bioactivity |
| topic | diversity antimicrobial antioxidant bioprospecting Africa endophyte |
| url | https://pbsociety.org.pl/journals/index.php/am/article/view/8093 |
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